Molecular Assessment of <i>MCR</i>-1 Gene among Pandrug-Resistant <i>Acinetobacter baumannii</i>
Background Antimicrobial resistance has become one of the most widespread threats to humans. <i>Acinetobacter baumannii</i> is one of the pathogens responsible for healthcare-associated infections (nosocomial). Colistin is considered the last resort antibiotic against infections with pan...
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MDPI AG
2023-08-01
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| Series: | Microbiology Research |
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| Online Access: | https://www.mdpi.com/2036-7481/14/3/83 |
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| author | Shaimaa S. Sobieh Shereen A. H. Mohamed Manal A. El-Sayed Soad A. Abdallah |
| author_facet | Shaimaa S. Sobieh Shereen A. H. Mohamed Manal A. El-Sayed Soad A. Abdallah |
| author_sort | Shaimaa S. Sobieh |
| collection | DOAJ |
| description | Background Antimicrobial resistance has become one of the most widespread threats to humans. <i>Acinetobacter baumannii</i> is one of the pathogens responsible for healthcare-associated infections (nosocomial). Colistin is considered the last resort antibiotic against infections with pandrug-resistant (PDR) pathogens. Results: Eleven isolates were detected phenotypically as PDR <i>A. baumannii</i> and were confirmed molecularly using 16S rDNA. The <i>MCR</i>-1 gene was not detected within the chromosomal DNA of the selected isolates. Plasmid bearing the <i>MCR</i>-1 gene was identified in 10 selected isolates of <i>A. baumannii</i> that had not been previously observed to carry the <i>MCR</i>-1 gene. Moreover, the use of colistin in combination with anionic antibiotics or natural compound pterostilbene poses a viable therapeutic alternative for PDR and revives colistin’s bactericidal effects on <i>MCR</i>-1-positive <i>A. baumannii</i>. Finally, the transmission electron microscopy studies proved the synergistic effect of these combinations and revealed the disruption of resistant <i>A. baumannii</i>’s outer membrane and alteration of the permeability properties that allowed overcoming the resistance of the isolates to colistin. Conclusions: Antimicrobial resistance of <i>A. baumannii</i> is related to the presence of the transferable plasmid-bearing <i>MCR</i>-1 gene. This study proved the ability of the combinations of colistin with anionic antibiotics and/or natural compound pterostilbene to restore the bactericidal effect of colistin. Overall, these combinations could be novel promising clinical alternatives against the increasing threat of the widespread multidrug-resistant <i>A. baumannii</i>. |
| first_indexed | 2024-03-10T22:28:02Z |
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| language | English |
| last_indexed | 2024-03-10T22:28:02Z |
| publishDate | 2023-08-01 |
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| record_format | Article |
| series | Microbiology Research |
| spelling | doaj.art-7cf65cb513c14c2cac3310938389472d2023-11-19T11:58:27ZengMDPI AGMicrobiology Research2036-74812023-08-011431238125110.3390/microbiolres14030083Molecular Assessment of <i>MCR</i>-1 Gene among Pandrug-Resistant <i>Acinetobacter baumannii</i>Shaimaa S. Sobieh0Shereen A. H. Mohamed1Manal A. El-Sayed2Soad A. Abdallah3Botany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo 11511, EgyptMicrobial Genetics Department, National Research Centre, Cairo 11511, EgyptBotany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo 11511, EgyptBotany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo 11511, EgyptBackground Antimicrobial resistance has become one of the most widespread threats to humans. <i>Acinetobacter baumannii</i> is one of the pathogens responsible for healthcare-associated infections (nosocomial). Colistin is considered the last resort antibiotic against infections with pandrug-resistant (PDR) pathogens. Results: Eleven isolates were detected phenotypically as PDR <i>A. baumannii</i> and were confirmed molecularly using 16S rDNA. The <i>MCR</i>-1 gene was not detected within the chromosomal DNA of the selected isolates. Plasmid bearing the <i>MCR</i>-1 gene was identified in 10 selected isolates of <i>A. baumannii</i> that had not been previously observed to carry the <i>MCR</i>-1 gene. Moreover, the use of colistin in combination with anionic antibiotics or natural compound pterostilbene poses a viable therapeutic alternative for PDR and revives colistin’s bactericidal effects on <i>MCR</i>-1-positive <i>A. baumannii</i>. Finally, the transmission electron microscopy studies proved the synergistic effect of these combinations and revealed the disruption of resistant <i>A. baumannii</i>’s outer membrane and alteration of the permeability properties that allowed overcoming the resistance of the isolates to colistin. Conclusions: Antimicrobial resistance of <i>A. baumannii</i> is related to the presence of the transferable plasmid-bearing <i>MCR</i>-1 gene. This study proved the ability of the combinations of colistin with anionic antibiotics and/or natural compound pterostilbene to restore the bactericidal effect of colistin. Overall, these combinations could be novel promising clinical alternatives against the increasing threat of the widespread multidrug-resistant <i>A. baumannii</i>.https://www.mdpi.com/2036-7481/14/3/83<i>Acinetobacter baumannii</i>horizontal gene transfer<i>MCR</i>-1 genepandrug-resistantovercoming colistin resistance |
| spellingShingle | Shaimaa S. Sobieh Shereen A. H. Mohamed Manal A. El-Sayed Soad A. Abdallah Molecular Assessment of <i>MCR</i>-1 Gene among Pandrug-Resistant <i>Acinetobacter baumannii</i> Microbiology Research <i>Acinetobacter baumannii</i> horizontal gene transfer <i>MCR</i>-1 gene pandrug-resistant overcoming colistin resistance |
| title | Molecular Assessment of <i>MCR</i>-1 Gene among Pandrug-Resistant <i>Acinetobacter baumannii</i> |
| title_full | Molecular Assessment of <i>MCR</i>-1 Gene among Pandrug-Resistant <i>Acinetobacter baumannii</i> |
| title_fullStr | Molecular Assessment of <i>MCR</i>-1 Gene among Pandrug-Resistant <i>Acinetobacter baumannii</i> |
| title_full_unstemmed | Molecular Assessment of <i>MCR</i>-1 Gene among Pandrug-Resistant <i>Acinetobacter baumannii</i> |
| title_short | Molecular Assessment of <i>MCR</i>-1 Gene among Pandrug-Resistant <i>Acinetobacter baumannii</i> |
| title_sort | molecular assessment of i mcr i 1 gene among pandrug resistant i acinetobacter baumannii i |
| topic | <i>Acinetobacter baumannii</i> horizontal gene transfer <i>MCR</i>-1 gene pandrug-resistant overcoming colistin resistance |
| url | https://www.mdpi.com/2036-7481/14/3/83 |
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