Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis
Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Nucleus |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19491034.2022.2047289 |
| _version_ | 1819019069403168768 |
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| author | Ulrike Schweigel Petros Batsios Annette Müller-Taubenberger Ralph Gräf Marianne Grafe |
| author_facet | Ulrike Schweigel Petros Batsios Annette Müller-Taubenberger Ralph Gräf Marianne Grafe |
| author_sort | Ulrike Schweigel |
| collection | DOAJ |
| description | Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae. |
| first_indexed | 2024-12-21T03:29:27Z |
| format | Article |
| id | doaj.art-7cf6a4cf992f4c1fb5edb645408ab479 |
| institution | Directory Open Access Journal |
| issn | 1949-1034 1949-1042 |
| language | English |
| last_indexed | 2024-12-21T03:29:27Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Nucleus |
| spelling | doaj.art-7cf6a4cf992f4c1fb5edb645408ab4792022-12-21T19:17:31ZengTaylor & Francis GroupNucleus1949-10341949-10422022-12-0113114415310.1080/19491034.2022.2047289Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosisUlrike Schweigel0Petros Batsios1Annette Müller-Taubenberger2Ralph Gräf3Marianne Grafe4Department of Cell Biology, University of Potsdam, Institute for Biochemistry and Biology, Potsdam-Golm, GermanyDepartment of Cell Biology, University of Potsdam, Institute for Biochemistry and Biology, Potsdam-Golm, GermanyDepartment of Cell Biology, LMU Munich, Biomedical Center, Planegg-Martinsried, GermanyDepartment of Cell Biology, University of Potsdam, Institute for Biochemistry and Biology, Potsdam-Golm, GermanyDepartment of Cell Biology, University of Potsdam, Institute for Biochemistry and Biology, Potsdam-Golm, GermanyDictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae.https://www.tandfonline.com/doi/10.1080/19491034.2022.2047289SpastinLEM-domainESCRTsun1dictyosteliumnuclear envelope |
| spellingShingle | Ulrike Schweigel Petros Batsios Annette Müller-Taubenberger Ralph Gräf Marianne Grafe Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis Nucleus Spastin LEM-domain ESCRT sun1 dictyostelium nuclear envelope |
| title | Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis |
| title_full | Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis |
| title_fullStr | Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis |
| title_full_unstemmed | Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis |
| title_short | Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis |
| title_sort | dictyostelium spastin is involved in nuclear envelope dynamics during semi closed mitosis |
| topic | Spastin LEM-domain ESCRT sun1 dictyostelium nuclear envelope |
| url | https://www.tandfonline.com/doi/10.1080/19491034.2022.2047289 |
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