Determinants of the Vertical Distribution of the Phyllosphere Differ Between Microbial Groups and the Epi- and Endosphere in a Tropical Forest
The determinants of phyllosphere microbial communities are drawing much attention given their functional importance for their plant host fitness and health. Identifying these determinants remains challenging in neotropical forests, considering the diversity of the tree hosts and the strong vertical...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2023-11-01
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| Series: | Phytobiomes Journal |
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-02-23-0013-R |
| _version_ | 1797606218686005248 |
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| author | Heidy Schimann Corinne Vacher Sabrina Coste Eliane Louisanna Tania Fort Lucie Zinger |
| author_facet | Heidy Schimann Corinne Vacher Sabrina Coste Eliane Louisanna Tania Fort Lucie Zinger |
| author_sort | Heidy Schimann |
| collection | DOAJ |
| description | The determinants of phyllosphere microbial communities are drawing much attention given their functional importance for their plant host fitness and health. Identifying these determinants remains challenging in neotropical forests, considering the diversity of the tree hosts and the strong vertical heterogeneity of abiotic conditions within the canopy and at the scale of the leaf. Here, we studied fungal and bacterial communities living in the endophytic and epiphytic phyllosphere in tree species across vertical gradients, from the top of the canopy to the ground. We used DNA metabarcoding to characterize microbial communities and measured abiotic variables and foliar traits to characterize environmental heterogeneity. The assembly of fungal communities was more driven by deterministic processes compared with bacteria, with endo- and epiphytic communities being similarly shaped by the host identity and unmeasured parameters. In contrast, in bacterial communities, the relative importance of deterministic processes decreased from endophytic to epiphytic communities. Bacterial epi- and endophytic communities were partly and differently determined by the position within the canopy, the host identity, and leaf traits, suggesting an effect of the vertical gradient and a stronger selection in the inner tissues of the leaf than on its surface. The tree host exerts a selective pressure on microbial communities but the leaf as microhabitat also contributes significantly to the assembly of microbial communities. Discrepancies exist between fungi and bacteria that probably reflect different life-history traits and ecological strategies, emphasizing the need to study these communities jointly if we are to fully understand plant–phyllosphere interactions. [Graphic: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. |
| first_indexed | 2024-03-11T05:12:04Z |
| format | Article |
| id | doaj.art-7cf6d318fbad420bb276708451c85dcd |
| institution | Directory Open Access Journal |
| issn | 2471-2906 |
| language | English |
| last_indexed | 2024-03-11T05:12:04Z |
| publishDate | 2023-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Phytobiomes Journal |
| spelling | doaj.art-7cf6d318fbad420bb276708451c85dcd2023-11-17T18:31:54ZengThe American Phytopathological SocietyPhytobiomes Journal2471-29062023-11-017331232310.1094/PBIOMES-02-23-0013-RDeterminants of the Vertical Distribution of the Phyllosphere Differ Between Microbial Groups and the Epi- and Endosphere in a Tropical ForestHeidy Schimann0Corinne Vacher1Sabrina Coste2Eliane Louisanna3Tania Fort4Lucie Zinger5INRAE, Université de Bordeaux, BIOGECO, 33610, Cestas, FranceINRAE, Université de Bordeaux, BIOGECO, 33610, Cestas, FranceUniversité de Guyane, ECOFOG (APT, CIRAD, CNRS, INRAE, Université des Antilles), Kourou, FranceINRAE, ECOFOG (APT, CIRAD, CNRS, Université de Guyane, Université des Antilles), Kourou, FranceINRAE, Université de Bordeaux, BIOGECO, 33610, Cestas, FranceÉcole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l’École Normale Supérieure (IBENS), Paris, FranceThe determinants of phyllosphere microbial communities are drawing much attention given their functional importance for their plant host fitness and health. Identifying these determinants remains challenging in neotropical forests, considering the diversity of the tree hosts and the strong vertical heterogeneity of abiotic conditions within the canopy and at the scale of the leaf. Here, we studied fungal and bacterial communities living in the endophytic and epiphytic phyllosphere in tree species across vertical gradients, from the top of the canopy to the ground. We used DNA metabarcoding to characterize microbial communities and measured abiotic variables and foliar traits to characterize environmental heterogeneity. The assembly of fungal communities was more driven by deterministic processes compared with bacteria, with endo- and epiphytic communities being similarly shaped by the host identity and unmeasured parameters. In contrast, in bacterial communities, the relative importance of deterministic processes decreased from endophytic to epiphytic communities. Bacterial epi- and endophytic communities were partly and differently determined by the position within the canopy, the host identity, and leaf traits, suggesting an effect of the vertical gradient and a stronger selection in the inner tissues of the leaf than on its surface. The tree host exerts a selective pressure on microbial communities but the leaf as microhabitat also contributes significantly to the assembly of microbial communities. Discrepancies exist between fungi and bacteria that probably reflect different life-history traits and ecological strategies, emphasizing the need to study these communities jointly if we are to fully understand plant–phyllosphere interactions. [Graphic: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-02-23-0013-Rassembly rulesbacteriaendophytesepiphytesfungileaf traits |
| spellingShingle | Heidy Schimann Corinne Vacher Sabrina Coste Eliane Louisanna Tania Fort Lucie Zinger Determinants of the Vertical Distribution of the Phyllosphere Differ Between Microbial Groups and the Epi- and Endosphere in a Tropical Forest Phytobiomes Journal assembly rules bacteria endophytes epiphytes fungi leaf traits |
| title | Determinants of the Vertical Distribution of the Phyllosphere Differ Between Microbial Groups and the Epi- and Endosphere in a Tropical Forest |
| title_full | Determinants of the Vertical Distribution of the Phyllosphere Differ Between Microbial Groups and the Epi- and Endosphere in a Tropical Forest |
| title_fullStr | Determinants of the Vertical Distribution of the Phyllosphere Differ Between Microbial Groups and the Epi- and Endosphere in a Tropical Forest |
| title_full_unstemmed | Determinants of the Vertical Distribution of the Phyllosphere Differ Between Microbial Groups and the Epi- and Endosphere in a Tropical Forest |
| title_short | Determinants of the Vertical Distribution of the Phyllosphere Differ Between Microbial Groups and the Epi- and Endosphere in a Tropical Forest |
| title_sort | determinants of the vertical distribution of the phyllosphere differ between microbial groups and the epi and endosphere in a tropical forest |
| topic | assembly rules bacteria endophytes epiphytes fungi leaf traits |
| url | https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-02-23-0013-R |
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