Rapid Detection and Antimicrobial Susceptibility Testing of Pathogens Using AgNPs-Invertase Complexes and the Personal Glucose Meter
Rapid detection of pathogens and assessment of antimicrobial susceptibility is of great importance for public health, especially in resource-limiting regions. Herein, we developed a rapid, portable, and universal detection method for bacteria using AgNPs-invertase complexes and the personal glucose...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.795415/full |
| _version_ | 1798025367304273920 |
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| author | Laibao Zheng Yunqiu Shen Wenjia Dong Chaochuan Zheng Ruolan Zhou Yong-Liang Lou |
| author_facet | Laibao Zheng Yunqiu Shen Wenjia Dong Chaochuan Zheng Ruolan Zhou Yong-Liang Lou |
| author_sort | Laibao Zheng |
| collection | DOAJ |
| description | Rapid detection of pathogens and assessment of antimicrobial susceptibility is of great importance for public health, especially in resource-limiting regions. Herein, we developed a rapid, portable, and universal detection method for bacteria using AgNPs-invertase complexes and the personal glucose meter (PGM). In the presence of bacteria, the invertase could be released from AgNPs-invertase complexes where its enzyme activity of invertase was inhibited. Then, the enzyme activity of invertase was restored and could convert sucrose into glucose measured by a commercially PGM. There was a good linear relationship between PGM signal and concentration of E. coli or S. aureus as the bacteria model with high sensitivity. And our proposed biosensor was proved to be a rapid and reliable method for antimicrobial susceptibility testing within 4 h with consistent results of Minimum Inhibitory Concentrations (MICs) testing, providing a portable and convenient method to treat infected patients with correct antibiotics and reduce the production of antibiotic-resistant bacteria, especially for resource-limiting settings. |
| first_indexed | 2024-04-11T18:17:40Z |
| format | Article |
| id | doaj.art-7cf7de5144d94eb8bb9e1c3cf3f7533a |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T18:17:40Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-7cf7de5144d94eb8bb9e1c3cf3f7533a2022-12-22T04:09:50ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-01-01910.3389/fbioe.2021.795415795415Rapid Detection and Antimicrobial Susceptibility Testing of Pathogens Using AgNPs-Invertase Complexes and the Personal Glucose MeterLaibao ZhengYunqiu ShenWenjia DongChaochuan ZhengRuolan ZhouYong-Liang LouRapid detection of pathogens and assessment of antimicrobial susceptibility is of great importance for public health, especially in resource-limiting regions. Herein, we developed a rapid, portable, and universal detection method for bacteria using AgNPs-invertase complexes and the personal glucose meter (PGM). In the presence of bacteria, the invertase could be released from AgNPs-invertase complexes where its enzyme activity of invertase was inhibited. Then, the enzyme activity of invertase was restored and could convert sucrose into glucose measured by a commercially PGM. There was a good linear relationship between PGM signal and concentration of E. coli or S. aureus as the bacteria model with high sensitivity. And our proposed biosensor was proved to be a rapid and reliable method for antimicrobial susceptibility testing within 4 h with consistent results of Minimum Inhibitory Concentrations (MICs) testing, providing a portable and convenient method to treat infected patients with correct antibiotics and reduce the production of antibiotic-resistant bacteria, especially for resource-limiting settings.https://www.frontiersin.org/articles/10.3389/fbioe.2021.795415/fullnanoparticle-enzyme complexespersonal glucose meterpathogen detectionantimicrobial susceptibility testingrapid detection |
| spellingShingle | Laibao Zheng Yunqiu Shen Wenjia Dong Chaochuan Zheng Ruolan Zhou Yong-Liang Lou Rapid Detection and Antimicrobial Susceptibility Testing of Pathogens Using AgNPs-Invertase Complexes and the Personal Glucose Meter Frontiers in Bioengineering and Biotechnology nanoparticle-enzyme complexes personal glucose meter pathogen detection antimicrobial susceptibility testing rapid detection |
| title | Rapid Detection and Antimicrobial Susceptibility Testing of Pathogens Using AgNPs-Invertase Complexes and the Personal Glucose Meter |
| title_full | Rapid Detection and Antimicrobial Susceptibility Testing of Pathogens Using AgNPs-Invertase Complexes and the Personal Glucose Meter |
| title_fullStr | Rapid Detection and Antimicrobial Susceptibility Testing of Pathogens Using AgNPs-Invertase Complexes and the Personal Glucose Meter |
| title_full_unstemmed | Rapid Detection and Antimicrobial Susceptibility Testing of Pathogens Using AgNPs-Invertase Complexes and the Personal Glucose Meter |
| title_short | Rapid Detection and Antimicrobial Susceptibility Testing of Pathogens Using AgNPs-Invertase Complexes and the Personal Glucose Meter |
| title_sort | rapid detection and antimicrobial susceptibility testing of pathogens using agnps invertase complexes and the personal glucose meter |
| topic | nanoparticle-enzyme complexes personal glucose meter pathogen detection antimicrobial susceptibility testing rapid detection |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.795415/full |
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