Role of CD5 molecular-like on hepatocellular carcinoma
Abstract. Background:. CD5L (CD5 molecular-like) plays an important role in lipid metabolism and immune regulation. This study aimed to investigate the roles of CD5L on liver hepatocellular carcinoma (LIHC). Methods:. We analyzed the CD5L mRNA expression and its potential prognostic value based on T...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2023-03-01
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| Series: | Chinese Medical Journal |
| Online Access: | http://journals.lww.com/10.1097/CM9.0000000000002576 |
| _version_ | 1797840213595127808 |
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| author | Zhaowei Gao Xianan Wu Lan Yang Chong Liu Xi Wang Huiping Wang Ke Dong Yuanyuan Ji |
| author_facet | Zhaowei Gao Xianan Wu Lan Yang Chong Liu Xi Wang Huiping Wang Ke Dong Yuanyuan Ji |
| author_sort | Zhaowei Gao |
| collection | DOAJ |
| description | Abstract. Background:. CD5L (CD5 molecular-like) plays an important role in lipid metabolism and immune regulation. This study aimed to investigate the roles of CD5L on liver hepatocellular carcinoma (LIHC).
Methods:. We analyzed the CD5L mRNA expression and its potential prognostic value based on The Cancer Genome Atlas and Gene Expression Omnibus databases. Immunohistochemical analysis was used to investigate the CD5L levels in LIHC tissues. Serum CD5L levels in LIHC were detected by enzyme-linked immunosorbent assay. Cell Counting Kit-8 (CCK-8) assay was used to investigate the effect of CD5L treatment on HepG2 and QSG-7701 cell proliferation. CD5L expression correlated genes were exhumed based on the LinkedOmics. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses for CD5L associated genes were performed. The correlation between CD5L and tumor immune infiltration was analyzed by using Tumor Immune Estimation Resource (TIMER) 2.0.
Results:. CD5L mRNA and protein levels were significantly decreased in LIHC tumor tissue compared with non-tumor control tissues. Moreover, serum CD5L levels were significantly lower in LIHC patients than that in healthy subjects. Gene Expression Profiling Interactive Analysis 2 and Kaplan-Meier plotter analysis showed that a high-CD5L expression was correlated with favorable overall survival in LIHC patients, except the LIHC patients with hepatitis virus. CCK-8 results showed that CD5L treatment significantly decreased HepG2 cell proliferation in a concentration-dependent manner, and CD5L treatment had no effect on the proliferation of non-tumor hepatocyte line QSG-7701. CD5L associated genes were enriched in the immune response biological process, and CD5L expression levels were positively correlated with the immune infiltrates of CD8+ T cell and M1 macrophage cells but negatively correlated with CD4+ T cells and M0 macrophage cell infiltration.
Conclusions:. Exogenous CD5L inhibits cell proliferation of hepatocellular carcinoma. CD5L may act as a role of prognostic marker. |
| first_indexed | 2024-04-09T16:11:16Z |
| format | Article |
| id | doaj.art-7cfa2513e45740b99e2d4e08f9d52678 |
| institution | Directory Open Access Journal |
| issn | 0366-6999 2542-5641 |
| language | English |
| last_indexed | 2024-04-09T16:11:16Z |
| publishDate | 2023-03-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-7cfa2513e45740b99e2d4e08f9d526782023-04-24T10:07:12ZengWolters KluwerChinese Medical Journal0366-69992542-56412023-03-01136555656410.1097/CM9.0000000000002576202303050-00008Role of CD5 molecular-like on hepatocellular carcinomaZhaowei GaoXianan WuLan YangChong LiuXi WangHuiping WangKe DongYuanyuan JiAbstract. Background:. CD5L (CD5 molecular-like) plays an important role in lipid metabolism and immune regulation. This study aimed to investigate the roles of CD5L on liver hepatocellular carcinoma (LIHC). Methods:. We analyzed the CD5L mRNA expression and its potential prognostic value based on The Cancer Genome Atlas and Gene Expression Omnibus databases. Immunohistochemical analysis was used to investigate the CD5L levels in LIHC tissues. Serum CD5L levels in LIHC were detected by enzyme-linked immunosorbent assay. Cell Counting Kit-8 (CCK-8) assay was used to investigate the effect of CD5L treatment on HepG2 and QSG-7701 cell proliferation. CD5L expression correlated genes were exhumed based on the LinkedOmics. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses for CD5L associated genes were performed. The correlation between CD5L and tumor immune infiltration was analyzed by using Tumor Immune Estimation Resource (TIMER) 2.0. Results:. CD5L mRNA and protein levels were significantly decreased in LIHC tumor tissue compared with non-tumor control tissues. Moreover, serum CD5L levels were significantly lower in LIHC patients than that in healthy subjects. Gene Expression Profiling Interactive Analysis 2 and Kaplan-Meier plotter analysis showed that a high-CD5L expression was correlated with favorable overall survival in LIHC patients, except the LIHC patients with hepatitis virus. CCK-8 results showed that CD5L treatment significantly decreased HepG2 cell proliferation in a concentration-dependent manner, and CD5L treatment had no effect on the proliferation of non-tumor hepatocyte line QSG-7701. CD5L associated genes were enriched in the immune response biological process, and CD5L expression levels were positively correlated with the immune infiltrates of CD8+ T cell and M1 macrophage cells but negatively correlated with CD4+ T cells and M0 macrophage cell infiltration. Conclusions:. Exogenous CD5L inhibits cell proliferation of hepatocellular carcinoma. CD5L may act as a role of prognostic marker.http://journals.lww.com/10.1097/CM9.0000000000002576 |
| spellingShingle | Zhaowei Gao Xianan Wu Lan Yang Chong Liu Xi Wang Huiping Wang Ke Dong Yuanyuan Ji Role of CD5 molecular-like on hepatocellular carcinoma Chinese Medical Journal |
| title | Role of CD5 molecular-like on hepatocellular carcinoma |
| title_full | Role of CD5 molecular-like on hepatocellular carcinoma |
| title_fullStr | Role of CD5 molecular-like on hepatocellular carcinoma |
| title_full_unstemmed | Role of CD5 molecular-like on hepatocellular carcinoma |
| title_short | Role of CD5 molecular-like on hepatocellular carcinoma |
| title_sort | role of cd5 molecular like on hepatocellular carcinoma |
| url | http://journals.lww.com/10.1097/CM9.0000000000002576 |
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