Counting cells in motion by quantitative real-time magnetic particle imaging
Abstract Magnetic Particle Imaging (MPI) is an advanced and powerful imaging modality for visualization and quantitative real-time detection of magnetic nanoparticles (MNPs). This opens the possibility of tracking cells in vivo once they have been loaded by MNPs. Imaging modalities such as optical i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-54784-5 |
| _version_ | 1797275151025307648 |
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| author | Amani Remmo Olaf Kosch Lena Kampen Antje Ludwig Frank Wiekhorst Norbert Löwa |
| author_facet | Amani Remmo Olaf Kosch Lena Kampen Antje Ludwig Frank Wiekhorst Norbert Löwa |
| author_sort | Amani Remmo |
| collection | DOAJ |
| description | Abstract Magnetic Particle Imaging (MPI) is an advanced and powerful imaging modality for visualization and quantitative real-time detection of magnetic nanoparticles (MNPs). This opens the possibility of tracking cells in vivo once they have been loaded by MNPs. Imaging modalities such as optical imaging, X-ray computed tomography (CT), positron emission tomography (PET), single photon emission computed tomography (SPECT), and magnetic resonance imaging (MRI) face limitations, from depth of penetration and radiation exposure to resolution and quantification accuracy. MPI addresses these challenges, enabling radiation-free tracking of MNP-loaded cells with precise quantification. However, the real-time tracking of MNP-loaded cells with MPI has not been demonstrated yet. This study establishes real-time quantitative tracking of MNP-loaded cells. Therefore, THP-1 monocytes were loaded with three different MNP systems, including the MPI gold standard Resovist and Synomag. The real-time MPI experiments reveal different MPI resolution behaviors of the three MNP systems after cellular uptake. Real-time quantitative imaging was achieved by time-resolved cell number determination and comparison with the number of inserted cells. About 95% of the inserted cells were successfully tracked in a controlled phantom environment. These results underline the potential of MPI for real-time investigation of cell migration and interaction with tissue in vivo. |
| first_indexed | 2024-03-07T15:09:11Z |
| format | Article |
| id | doaj.art-7cfe9d6efc4d44f2a42b0d2ce2fd87cd |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:09:11Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7cfe9d6efc4d44f2a42b0d2ce2fd87cd2024-03-05T18:44:33ZengNature PortfolioScientific Reports2045-23222024-02-0114111010.1038/s41598-024-54784-5Counting cells in motion by quantitative real-time magnetic particle imagingAmani Remmo0Olaf Kosch1Lena Kampen2Antje Ludwig3Frank Wiekhorst4Norbert Löwa5Physikalisch-Technische BundesanstaltPhysikalisch-Technische BundesanstaltDepartment of Cardiology, Angiology and Intensive Care Medicine, Deutsches Herzzentrum der CharitéDepartment of Cardiology, Angiology and Intensive Care Medicine, Deutsches Herzzentrum der CharitéPhysikalisch-Technische BundesanstaltPhysikalisch-Technische BundesanstaltAbstract Magnetic Particle Imaging (MPI) is an advanced and powerful imaging modality for visualization and quantitative real-time detection of magnetic nanoparticles (MNPs). This opens the possibility of tracking cells in vivo once they have been loaded by MNPs. Imaging modalities such as optical imaging, X-ray computed tomography (CT), positron emission tomography (PET), single photon emission computed tomography (SPECT), and magnetic resonance imaging (MRI) face limitations, from depth of penetration and radiation exposure to resolution and quantification accuracy. MPI addresses these challenges, enabling radiation-free tracking of MNP-loaded cells with precise quantification. However, the real-time tracking of MNP-loaded cells with MPI has not been demonstrated yet. This study establishes real-time quantitative tracking of MNP-loaded cells. Therefore, THP-1 monocytes were loaded with three different MNP systems, including the MPI gold standard Resovist and Synomag. The real-time MPI experiments reveal different MPI resolution behaviors of the three MNP systems after cellular uptake. Real-time quantitative imaging was achieved by time-resolved cell number determination and comparison with the number of inserted cells. About 95% of the inserted cells were successfully tracked in a controlled phantom environment. These results underline the potential of MPI for real-time investigation of cell migration and interaction with tissue in vivo.https://doi.org/10.1038/s41598-024-54784-5 |
| spellingShingle | Amani Remmo Olaf Kosch Lena Kampen Antje Ludwig Frank Wiekhorst Norbert Löwa Counting cells in motion by quantitative real-time magnetic particle imaging Scientific Reports |
| title | Counting cells in motion by quantitative real-time magnetic particle imaging |
| title_full | Counting cells in motion by quantitative real-time magnetic particle imaging |
| title_fullStr | Counting cells in motion by quantitative real-time magnetic particle imaging |
| title_full_unstemmed | Counting cells in motion by quantitative real-time magnetic particle imaging |
| title_short | Counting cells in motion by quantitative real-time magnetic particle imaging |
| title_sort | counting cells in motion by quantitative real time magnetic particle imaging |
| url | https://doi.org/10.1038/s41598-024-54784-5 |
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