Is blue light exposure a cause of precocious puberty in male rats?
PurposeOur study aimed to examine the effects of blue light exposure on prepubertal male rats’ puberty and testis tissue.MethodsEighteen 21-day-old male Sprague Dawley rats were divided into three groups consisting of six rats in each group: Control Group (CG), Blue Light-6 hours (BL-6), and Blue Li...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1190445/full |
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| author | Aylin Kılınç Uğurlu Aysun Bideci Ayşe Mürşide Demirel Gülnur Take Kaplanoğlu Duygu Dayanır Özlem Gülbahar Tuba Saadet Deveci Bulut Esra Döğer Mahmut Orhun Çamurdan |
| author_facet | Aylin Kılınç Uğurlu Aysun Bideci Ayşe Mürşide Demirel Gülnur Take Kaplanoğlu Duygu Dayanır Özlem Gülbahar Tuba Saadet Deveci Bulut Esra Döğer Mahmut Orhun Çamurdan |
| author_sort | Aylin Kılınç Uğurlu |
| collection | DOAJ |
| description | PurposeOur study aimed to examine the effects of blue light exposure on prepubertal male rats’ puberty and testis tissue.MethodsEighteen 21-day-old male Sprague Dawley rats were divided into three groups consisting of six rats in each group: Control Group (CG), Blue Light-6 hours (BL-6), and Blue Light-12 hours (BL-12). CG rats were maintained with 12/12-hour light-dark cycles. The rats of BL-6 and BL-12 were exposed to blue light (450-470nm/irradiance level 0.03uW/cm2) for 6 hours and 12 hours, respectively. Rats were exposed to blue light until the first signs of puberty. The ELISA method was used to analyze the serum levels of FSH, LH, testosterone, DHEA-S, leptin, ghrelin, melatonin, glutathione, glutathione peroxidase, and malondialdehyde. Testes were dissected for histomorphological examination.ResultsThe medians of the pubertal entry days of the CG, BL-6, and BL-12 were 38th, 30th, and 28th days, respectively. (p:0.001) The FSH, LH, and testosterone concentrations of all groups were similar. The FSH concentration increased as the LH concentration increased (r: 0.82 p: 0.001). The serum LH concentration increased as serum testosterone, and DHEAS decreased, respectively (r: -0.561, p: 0.01) (r:-0.55 p:0.01). Testicular lengths and weights of the BL groups were smaller compared to CG (p: 0.03),(p: 0.04). GPx was higher for BL-6 and BL-12 than the CG (p:0.021, p:0.024). Testis tissue was compatible with the pubertal period in all groups. As the blue light exposure time increased, spermatogenesis was suppressed, and capillary dilatation and edema in the testis tissue increased.ConclusionOur study is the first to show the effects of blue light exposure on male rats’ puberty process. And we showed that exposure to blue light and the duration of exposure lead to precocious puberty in male rats. The blue light exposure suppressed spermatogenesis, marked vasodilatation in the interstitial area of the testis, and disrupted the integrity of the basement membrane. These findings intensified with increasing exposure time. |
| first_indexed | 2024-03-13T04:20:50Z |
| format | Article |
| id | doaj.art-7d02c855d8d4468f9988f4f5eb1b7d3b |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-13T04:20:50Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-7d02c855d8d4468f9988f4f5eb1b7d3b2023-06-20T10:14:33ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-06-011410.3389/fendo.2023.11904451190445Is blue light exposure a cause of precocious puberty in male rats?Aylin Kılınç Uğurlu0Aysun Bideci1Ayşe Mürşide Demirel2Gülnur Take Kaplanoğlu3Duygu Dayanır4Özlem Gülbahar5Tuba Saadet Deveci Bulut6Esra Döğer7Mahmut Orhun Çamurdan8Ankara City Hospital, Pediatric Endocrinology Clinic, Ankara, TürkiyeDepartment of Pediatric Endocrinology, Faculty of Medicine, Gazi University, Ankara, TürkiyeDepartment of Basic Pharmaceutical Sciences Faculty of Pharmacy, Laboratory Animals Breeding and Experimental Research Center, Faculty of Pharmacy, Gazi University, Ankara, TürkiyeDepartment of Histology and Embryology, Faculty of Medicine, Gazi University, Ankara, TürkiyeDepartment of Histology and Embryology, Faculty of Medicine, Gazi University, Ankara, TürkiyeDepartment of Medical Biochemistry, Faculty of Medicine, Gazi University, Ankara, TürkiyeDepartment of Medical Biochemistry, Faculty of Medicine, Gazi University, Ankara, TürkiyeDepartment of Pediatric Endocrinology, Faculty of Medicine, Gazi University, Ankara, TürkiyeDepartment of Pediatric Endocrinology, Faculty of Medicine, Gazi University, Ankara, TürkiyePurposeOur study aimed to examine the effects of blue light exposure on prepubertal male rats’ puberty and testis tissue.MethodsEighteen 21-day-old male Sprague Dawley rats were divided into three groups consisting of six rats in each group: Control Group (CG), Blue Light-6 hours (BL-6), and Blue Light-12 hours (BL-12). CG rats were maintained with 12/12-hour light-dark cycles. The rats of BL-6 and BL-12 were exposed to blue light (450-470nm/irradiance level 0.03uW/cm2) for 6 hours and 12 hours, respectively. Rats were exposed to blue light until the first signs of puberty. The ELISA method was used to analyze the serum levels of FSH, LH, testosterone, DHEA-S, leptin, ghrelin, melatonin, glutathione, glutathione peroxidase, and malondialdehyde. Testes were dissected for histomorphological examination.ResultsThe medians of the pubertal entry days of the CG, BL-6, and BL-12 were 38th, 30th, and 28th days, respectively. (p:0.001) The FSH, LH, and testosterone concentrations of all groups were similar. The FSH concentration increased as the LH concentration increased (r: 0.82 p: 0.001). The serum LH concentration increased as serum testosterone, and DHEAS decreased, respectively (r: -0.561, p: 0.01) (r:-0.55 p:0.01). Testicular lengths and weights of the BL groups were smaller compared to CG (p: 0.03),(p: 0.04). GPx was higher for BL-6 and BL-12 than the CG (p:0.021, p:0.024). Testis tissue was compatible with the pubertal period in all groups. As the blue light exposure time increased, spermatogenesis was suppressed, and capillary dilatation and edema in the testis tissue increased.ConclusionOur study is the first to show the effects of blue light exposure on male rats’ puberty process. And we showed that exposure to blue light and the duration of exposure lead to precocious puberty in male rats. The blue light exposure suppressed spermatogenesis, marked vasodilatation in the interstitial area of the testis, and disrupted the integrity of the basement membrane. These findings intensified with increasing exposure time.https://www.frontiersin.org/articles/10.3389/fendo.2023.1190445/fullblue light (470 nm)precocious pubertymale ratspermatogenesistestis damage |
| spellingShingle | Aylin Kılınç Uğurlu Aysun Bideci Ayşe Mürşide Demirel Gülnur Take Kaplanoğlu Duygu Dayanır Özlem Gülbahar Tuba Saadet Deveci Bulut Esra Döğer Mahmut Orhun Çamurdan Is blue light exposure a cause of precocious puberty in male rats? Frontiers in Endocrinology blue light (470 nm) precocious puberty male rat spermatogenesis testis damage |
| title | Is blue light exposure a cause of precocious puberty in male rats? |
| title_full | Is blue light exposure a cause of precocious puberty in male rats? |
| title_fullStr | Is blue light exposure a cause of precocious puberty in male rats? |
| title_full_unstemmed | Is blue light exposure a cause of precocious puberty in male rats? |
| title_short | Is blue light exposure a cause of precocious puberty in male rats? |
| title_sort | is blue light exposure a cause of precocious puberty in male rats |
| topic | blue light (470 nm) precocious puberty male rat spermatogenesis testis damage |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1190445/full |
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