Biodiversity of New Lytic Bacteriophages Infecting Shigella spp. in Freshwater Environment
Bacteriophages, viruses that infect and replicate within prokaryotic cells are the most abundant life forms in the environment, yet the vast majority of them have not been properly reported or even discovered. Almost all reported bacteriophages infecting the Enterobacteriaceae family, with Escherich...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.619323/full |
| _version_ | 1819051115406163968 |
|---|---|
| author | Khashayar Shahin Mohadeseh Barazandeh Lili Zhang Abolghasem Hedayatkhah Tao He Hongduo Bao Mojtaba Mansoorianfar Maoda Pang Heye Wang Ruicheng Wei Ran Wang |
| author_facet | Khashayar Shahin Mohadeseh Barazandeh Lili Zhang Abolghasem Hedayatkhah Tao He Hongduo Bao Mojtaba Mansoorianfar Maoda Pang Heye Wang Ruicheng Wei Ran Wang |
| author_sort | Khashayar Shahin |
| collection | DOAJ |
| description | Bacteriophages, viruses that infect and replicate within prokaryotic cells are the most abundant life forms in the environment, yet the vast majority of them have not been properly reported or even discovered. Almost all reported bacteriophages infecting the Enterobacteriaceae family, with Escherichia coli being the major subject of studies, have been isolated from wastewater, sewage, and effluent resources. In the present study, we focused on the distribution and biodiversity of Shigella phages in an aquatic ecosystem. While no Shigella bacteria was recovered from the Yangtze River, three lytic phages were isolated from this ecosystem and were subjected to biological, morphological, and genomic characteristics. Comparative genomics and phylogenetic analyses demonstrated that vB _SflM_004 isolate belongs to Myoviridae family, Felixounavirus genus of Ounavirinae subfamily, vB_SdyM_006 was classified under the same family, however, it is suggested to be in a new genus under Tevenvirinae subfamily with some other related bacteriophages. vB_SsoS_008 phage belongs to the Siphoviridae family, Tunavirus genus, Tunavirinae subfamily. The phages did not harbor any genes involved in the lysogenic cycles and showed a high temperature and pH stability. The biodiversity of the isolated phages highly suggests that continued isolation on non-model members of Enterobacteriaceae family is necessary to fully understand bacteriophage diversity in aquatic environments. |
| first_indexed | 2024-12-21T11:58:48Z |
| format | Article |
| id | doaj.art-7d02d4d0dc7e44248ac9d39c311d5a70 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T11:58:48Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7d02d4d0dc7e44248ac9d39c311d5a702022-12-21T19:04:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-02-011210.3389/fmicb.2021.619323619323Biodiversity of New Lytic Bacteriophages Infecting Shigella spp. in Freshwater EnvironmentKhashayar Shahin0Mohadeseh Barazandeh1Lili Zhang2Abolghasem Hedayatkhah3Tao He4Hongduo Bao5Mojtaba Mansoorianfar6Maoda Pang7Heye Wang8Ruicheng Wei9Ran Wang10Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Institute of Food Safety and Nutrition, Nanjing, ChinaKey Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Institute of Food Safety and Nutrition, Nanjing, ChinaKey Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Institute of Food Safety and Nutrition, Nanjing, ChinaCurtin Medical School, Curtin University, Perth, WA, AustraliaKey Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Institute of Food Safety and Nutrition, Nanjing, ChinaKey Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Institute of Food Safety and Nutrition, Nanjing, ChinaSuzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, ChinaKey Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Institute of Food Safety and Nutrition, Nanjing, ChinaKey Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Institute of Food Safety and Nutrition, Nanjing, ChinaKey Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Institute of Food Safety and Nutrition, Nanjing, ChinaKey Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Jiangsu Academy of Agricultural Sciences, Institute of Food Safety and Nutrition, Nanjing, ChinaBacteriophages, viruses that infect and replicate within prokaryotic cells are the most abundant life forms in the environment, yet the vast majority of them have not been properly reported or even discovered. Almost all reported bacteriophages infecting the Enterobacteriaceae family, with Escherichia coli being the major subject of studies, have been isolated from wastewater, sewage, and effluent resources. In the present study, we focused on the distribution and biodiversity of Shigella phages in an aquatic ecosystem. While no Shigella bacteria was recovered from the Yangtze River, three lytic phages were isolated from this ecosystem and were subjected to biological, morphological, and genomic characteristics. Comparative genomics and phylogenetic analyses demonstrated that vB _SflM_004 isolate belongs to Myoviridae family, Felixounavirus genus of Ounavirinae subfamily, vB_SdyM_006 was classified under the same family, however, it is suggested to be in a new genus under Tevenvirinae subfamily with some other related bacteriophages. vB_SsoS_008 phage belongs to the Siphoviridae family, Tunavirus genus, Tunavirinae subfamily. The phages did not harbor any genes involved in the lysogenic cycles and showed a high temperature and pH stability. The biodiversity of the isolated phages highly suggests that continued isolation on non-model members of Enterobacteriaceae family is necessary to fully understand bacteriophage diversity in aquatic environments.https://www.frontiersin.org/articles/10.3389/fmicb.2021.619323/fullwaterphagebiodiversitybacteriophageShigellaEnterobacteriaceae |
| spellingShingle | Khashayar Shahin Mohadeseh Barazandeh Lili Zhang Abolghasem Hedayatkhah Tao He Hongduo Bao Mojtaba Mansoorianfar Maoda Pang Heye Wang Ruicheng Wei Ran Wang Biodiversity of New Lytic Bacteriophages Infecting Shigella spp. in Freshwater Environment Frontiers in Microbiology water phage biodiversity bacteriophage Shigella Enterobacteriaceae |
| title | Biodiversity of New Lytic Bacteriophages Infecting Shigella spp. in Freshwater Environment |
| title_full | Biodiversity of New Lytic Bacteriophages Infecting Shigella spp. in Freshwater Environment |
| title_fullStr | Biodiversity of New Lytic Bacteriophages Infecting Shigella spp. in Freshwater Environment |
| title_full_unstemmed | Biodiversity of New Lytic Bacteriophages Infecting Shigella spp. in Freshwater Environment |
| title_short | Biodiversity of New Lytic Bacteriophages Infecting Shigella spp. in Freshwater Environment |
| title_sort | biodiversity of new lytic bacteriophages infecting shigella spp in freshwater environment |
| topic | water phage biodiversity bacteriophage Shigella Enterobacteriaceae |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.619323/full |
| work_keys_str_mv | AT khashayarshahin biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment AT mohadesehbarazandeh biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment AT lilizhang biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment AT abolghasemhedayatkhah biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment AT taohe biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment AT hongduobao biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment AT mojtabamansoorianfar biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment AT maodapang biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment AT heyewang biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment AT ruichengwei biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment AT ranwang biodiversityofnewlyticbacteriophagesinfectingshigellasppinfreshwaterenvironment |