Punicalagin improves chorioallantoic and yolk sac vasculogenesis and teratogenesis of embryos induced by nicotine exposure
Punicalagin is a functional ingredient in pomegranate juice, which has various beneficial effects for health and prevention of disease. In this study, the anti-teratogenic effect of punicalagin (1 × 10−5 or 1 × 10−4 µM) was investigated in cultured mouse embryos and yolk sac-placentas exposed to ni...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-10-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464615004144 |
| _version_ | 1818418315597447168 |
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| author | Chunmei Lin Jung-Min Yon Beom Jun Lee Jong-Koo Kang Young Won Yun Sang-Yoon Nam |
| author_facet | Chunmei Lin Jung-Min Yon Beom Jun Lee Jong-Koo Kang Young Won Yun Sang-Yoon Nam |
| author_sort | Chunmei Lin |
| collection | DOAJ |
| description | Punicalagin is a functional ingredient in pomegranate juice, which has various beneficial effects for health and prevention of disease. In this study, the anti-teratogenic effect of punicalagin (1 × 10−5 or 1 × 10−4 µM) was investigated in cultured mouse embryos and yolk sac-placentas exposed to nicotine (1 mM), one of the main toxins in cigarette smoke. Nicotine-treated embryos revealed severe anomalies as well as impaired yolk sac vascularization, reduced labyrinth formation, and developmental arrest of blood islands in the chorioallantoic border. Furthermore, nicotine significantly altered the regulations of hypoxia- and vascularization-related genes in yolk sac-placenta and the levels of oxidative stress, apoptosis, and inflammation in both embryos and yolk sac-placentas. However, these detrimental changes were remarkably improved in response to co-treatment with punicalagins. These findings indicate that punicalagin could be crucial to the development of clinical strategies to attenuate nicotine-induced teratogenesis in embryos. |
| first_indexed | 2024-12-14T12:20:44Z |
| format | Article |
| id | doaj.art-7d086bf1952a43bb9fb7c638bc810555 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T12:20:44Z |
| publishDate | 2015-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-7d086bf1952a43bb9fb7c638bc8105552022-12-21T23:01:28ZengElsevierJournal of Functional Foods1756-46462015-10-0118617630Punicalagin improves chorioallantoic and yolk sac vasculogenesis and teratogenesis of embryos induced by nicotine exposureChunmei Lin0Jung-Min Yon1Beom Jun Lee2Jong-Koo Kang3Young Won Yun4Sang-Yoon Nam5College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 28644, South KoreaCollege of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 28644, South KoreaCollege of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 28644, South KoreaCollege of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 28644, South KoreaCollege of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 28644, South KoreaCorresponding author. Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, South Korea. Tel.: +82 43 261 2596; fax: +82 43 271 3246.; College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 28644, South KoreaPunicalagin is a functional ingredient in pomegranate juice, which has various beneficial effects for health and prevention of disease. In this study, the anti-teratogenic effect of punicalagin (1 × 10−5 or 1 × 10−4 µM) was investigated in cultured mouse embryos and yolk sac-placentas exposed to nicotine (1 mM), one of the main toxins in cigarette smoke. Nicotine-treated embryos revealed severe anomalies as well as impaired yolk sac vascularization, reduced labyrinth formation, and developmental arrest of blood islands in the chorioallantoic border. Furthermore, nicotine significantly altered the regulations of hypoxia- and vascularization-related genes in yolk sac-placenta and the levels of oxidative stress, apoptosis, and inflammation in both embryos and yolk sac-placentas. However, these detrimental changes were remarkably improved in response to co-treatment with punicalagins. These findings indicate that punicalagin could be crucial to the development of clinical strategies to attenuate nicotine-induced teratogenesis in embryos.http://www.sciencedirect.com/science/article/pii/S1756464615004144PunicalaginNicotineTeratogenesisPlacentaYolk sacVascularization |
| spellingShingle | Chunmei Lin Jung-Min Yon Beom Jun Lee Jong-Koo Kang Young Won Yun Sang-Yoon Nam Punicalagin improves chorioallantoic and yolk sac vasculogenesis and teratogenesis of embryos induced by nicotine exposure Journal of Functional Foods Punicalagin Nicotine Teratogenesis Placenta Yolk sac Vascularization |
| title | Punicalagin improves chorioallantoic and yolk sac vasculogenesis and teratogenesis of embryos induced by nicotine exposure |
| title_full | Punicalagin improves chorioallantoic and yolk sac vasculogenesis and teratogenesis of embryos induced by nicotine exposure |
| title_fullStr | Punicalagin improves chorioallantoic and yolk sac vasculogenesis and teratogenesis of embryos induced by nicotine exposure |
| title_full_unstemmed | Punicalagin improves chorioallantoic and yolk sac vasculogenesis and teratogenesis of embryos induced by nicotine exposure |
| title_short | Punicalagin improves chorioallantoic and yolk sac vasculogenesis and teratogenesis of embryos induced by nicotine exposure |
| title_sort | punicalagin improves chorioallantoic and yolk sac vasculogenesis and teratogenesis of embryos induced by nicotine exposure |
| topic | Punicalagin Nicotine Teratogenesis Placenta Yolk sac Vascularization |
| url | http://www.sciencedirect.com/science/article/pii/S1756464615004144 |
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