Variability in gut mucosal secretory IgA in mice along a working day
Abstract Objective To assess the variability of secretory immunoglobulin A (S-IgA) in the lumen and feces of mice along a working day. Results Mice were maintained under a 12 h light–dark cycle, light period starting at 8 AM. S-IgA was determined in feces and intestinal content (after one or three w...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3213-0 |
| _version_ | 1818036962838183936 |
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| author | Patricia Burns Sofia Oddi Liliana Forzani Eduardo Tabacman Jorge Reinheimer Gabriel Vinderola |
| author_facet | Patricia Burns Sofia Oddi Liliana Forzani Eduardo Tabacman Jorge Reinheimer Gabriel Vinderola |
| author_sort | Patricia Burns |
| collection | DOAJ |
| description | Abstract Objective To assess the variability of secretory immunoglobulin A (S-IgA) in the lumen and feces of mice along a working day. Results Mice were maintained under a 12 h light–dark cycle, light period starting at 8 AM. S-IgA was determined in feces and intestinal content (after one or three washes) at three points along the day: at the beginning, in the middle and at the end of the light period (ELP). Significant reduction in the content of S-IgA in the small intestine fluid and in feces was observed at the end of the light cycle, which coincides with the end of a regular working day (8 PM) in any given animal facility. It was also observed that three washes of the small intestine were more effective than one flush to recover a significant higher amount of S-IgA, with the smallest coefficient of variation observed by the ELP. A smaller CV would imply a reduced number of animals needed to achieve the same meaningful results. The results may be useful when designing animal trials for the selection of probiotic candidates based on their capacity of activating S-IgA, since it would imply a more rational use of experimental animals. |
| first_indexed | 2024-12-10T07:19:17Z |
| format | Article |
| id | doaj.art-7d0898542b334cafb54e420336620b50 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-10T07:19:17Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-7d0898542b334cafb54e420336620b502022-12-22T01:57:51ZengBMCBMC Research Notes1756-05002018-02-011111510.1186/s13104-018-3213-0Variability in gut mucosal secretory IgA in mice along a working dayPatricia Burns0Sofia Oddi1Liliana Forzani2Eduardo Tabacman3Jorge Reinheimer4Gabriel Vinderola5Instituto de Lactología Industrial (INLAIN, UNL-CONICET), Facultad de Ingeniería Química, Universidad Nacional del LitoralInstituto de Lactología Industrial (INLAIN, UNL-CONICET), Facultad de Ingeniería Química, Universidad Nacional del LitoralDepartamento de Matemática, Facultad de Ingeniería Química, Universidad Nacional del LitoralSomalogic, IncInstituto de Lactología Industrial (INLAIN, UNL-CONICET), Facultad de Ingeniería Química, Universidad Nacional del LitoralInstituto de Lactología Industrial (INLAIN, UNL-CONICET), Facultad de Ingeniería Química, Universidad Nacional del LitoralAbstract Objective To assess the variability of secretory immunoglobulin A (S-IgA) in the lumen and feces of mice along a working day. Results Mice were maintained under a 12 h light–dark cycle, light period starting at 8 AM. S-IgA was determined in feces and intestinal content (after one or three washes) at three points along the day: at the beginning, in the middle and at the end of the light period (ELP). Significant reduction in the content of S-IgA in the small intestine fluid and in feces was observed at the end of the light cycle, which coincides with the end of a regular working day (8 PM) in any given animal facility. It was also observed that three washes of the small intestine were more effective than one flush to recover a significant higher amount of S-IgA, with the smallest coefficient of variation observed by the ELP. A smaller CV would imply a reduced number of animals needed to achieve the same meaningful results. The results may be useful when designing animal trials for the selection of probiotic candidates based on their capacity of activating S-IgA, since it would imply a more rational use of experimental animals.http://link.springer.com/article/10.1186/s13104-018-3213-0IgAIntestineCircadian rhythmMiceVariability |
| spellingShingle | Patricia Burns Sofia Oddi Liliana Forzani Eduardo Tabacman Jorge Reinheimer Gabriel Vinderola Variability in gut mucosal secretory IgA in mice along a working day BMC Research Notes IgA Intestine Circadian rhythm Mice Variability |
| title | Variability in gut mucosal secretory IgA in mice along a working day |
| title_full | Variability in gut mucosal secretory IgA in mice along a working day |
| title_fullStr | Variability in gut mucosal secretory IgA in mice along a working day |
| title_full_unstemmed | Variability in gut mucosal secretory IgA in mice along a working day |
| title_short | Variability in gut mucosal secretory IgA in mice along a working day |
| title_sort | variability in gut mucosal secretory iga in mice along a working day |
| topic | IgA Intestine Circadian rhythm Mice Variability |
| url | http://link.springer.com/article/10.1186/s13104-018-3213-0 |
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