Phlorotannins of the Brown Algae <i>Sargassum vulgare</i> from the Mediterranean Sea Coast
Brown seaweeds are a good source of bioactive compounds, particularly of phlorotannins, which may exert a wide spectrum of pharmacological properties. In the present study, phlorotannins of <i>S. vulgare</i> were extracted using a 70% acetone solution and the crude extract was further pu...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/11/6/1055 |
| _version_ | 1827662907628847104 |
|---|---|
| author | Amina Chouh Tahar Nouadri Marcelo D. Catarino Artur M. S. Silva Susana M. Cardoso |
| author_facet | Amina Chouh Tahar Nouadri Marcelo D. Catarino Artur M. S. Silva Susana M. Cardoso |
| author_sort | Amina Chouh |
| collection | DOAJ |
| description | Brown seaweeds are a good source of bioactive compounds, particularly of phlorotannins, which may exert a wide spectrum of pharmacological properties. In the present study, phlorotannins of <i>S. vulgare</i> were extracted using a 70% acetone solution and the crude extract was further purified through liquid–liquid partition, giving rise to <i>n</i>-hexane, ethyl acetate and aqueous residue fractions. The crude extract and the purified fractions were evaluated for potential antioxidant abilities as well as for inhibitory potential towards the digestive enzymes α-amylase and pancreatic lipase, and anti-inflammatory potential through the hindering of albumin denaturation. Overall, the ethyl acetate fraction was the richest in phlorotannins (9.4 ± 0.03 mg PGE/g) and was also the most promising regarding the tested bioactive properties. Of note, its inhibitory potential towards α-amylase was about nine times that of the commercial drug acarbose and its inhibitory activity against high temperature-induced protein denaturation was superior to that of the non-steroidal drug ketoprofen. According to UHPLC-DAD-ESI-MS/MS analysis, this fraction contained a range of phlorotannins with at least six units of phloroglucinol, including dibenzodioxine-1,3,6,8-tetraol, fuhalol, pentaphlorethol, fucopentaphlorethol and dihydroxypentafuhalol, in addition to several less common phlorotannin sulfate derivatives. |
| first_indexed | 2024-03-10T00:34:30Z |
| format | Article |
| id | doaj.art-7d08cb4220954397820ab20641219ced |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T00:34:30Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-7d08cb4220954397820ab20641219ced2023-11-23T15:18:44ZengMDPI AGAntioxidants2076-39212022-05-01116105510.3390/antiox11061055Phlorotannins of the Brown Algae <i>Sargassum vulgare</i> from the Mediterranean Sea CoastAmina Chouh0Tahar Nouadri1Marcelo D. Catarino2Artur M. S. Silva3Susana M. Cardoso4Laboratory of Microbiological Engineering and Application, Department of Biochemistry and Molecular and Cellular Biology, Faculty of Nature and Life Sciences, University of Mentouri Brothers Constantine 1, Constantine 25017, AlgeriaLaboratory of Microbiological Engineering and Application, Department of Biochemistry and Molecular and Cellular Biology, Faculty of Nature and Life Sciences, University of Mentouri Brothers Constantine 1, Constantine 25017, AlgeriaLAQV-REQUIMTE & Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE & Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE & Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalBrown seaweeds are a good source of bioactive compounds, particularly of phlorotannins, which may exert a wide spectrum of pharmacological properties. In the present study, phlorotannins of <i>S. vulgare</i> were extracted using a 70% acetone solution and the crude extract was further purified through liquid–liquid partition, giving rise to <i>n</i>-hexane, ethyl acetate and aqueous residue fractions. The crude extract and the purified fractions were evaluated for potential antioxidant abilities as well as for inhibitory potential towards the digestive enzymes α-amylase and pancreatic lipase, and anti-inflammatory potential through the hindering of albumin denaturation. Overall, the ethyl acetate fraction was the richest in phlorotannins (9.4 ± 0.03 mg PGE/g) and was also the most promising regarding the tested bioactive properties. Of note, its inhibitory potential towards α-amylase was about nine times that of the commercial drug acarbose and its inhibitory activity against high temperature-induced protein denaturation was superior to that of the non-steroidal drug ketoprofen. According to UHPLC-DAD-ESI-MS/MS analysis, this fraction contained a range of phlorotannins with at least six units of phloroglucinol, including dibenzodioxine-1,3,6,8-tetraol, fuhalol, pentaphlorethol, fucopentaphlorethol and dihydroxypentafuhalol, in addition to several less common phlorotannin sulfate derivatives.https://www.mdpi.com/2076-3921/11/6/1055<i>Sargassum</i>antioxidantantidiabeticpancreatic lipaseanti-inflammatoryUHPLC-MS analysis |
| spellingShingle | Amina Chouh Tahar Nouadri Marcelo D. Catarino Artur M. S. Silva Susana M. Cardoso Phlorotannins of the Brown Algae <i>Sargassum vulgare</i> from the Mediterranean Sea Coast Antioxidants <i>Sargassum</i> antioxidant antidiabetic pancreatic lipase anti-inflammatory UHPLC-MS analysis |
| title | Phlorotannins of the Brown Algae <i>Sargassum vulgare</i> from the Mediterranean Sea Coast |
| title_full | Phlorotannins of the Brown Algae <i>Sargassum vulgare</i> from the Mediterranean Sea Coast |
| title_fullStr | Phlorotannins of the Brown Algae <i>Sargassum vulgare</i> from the Mediterranean Sea Coast |
| title_full_unstemmed | Phlorotannins of the Brown Algae <i>Sargassum vulgare</i> from the Mediterranean Sea Coast |
| title_short | Phlorotannins of the Brown Algae <i>Sargassum vulgare</i> from the Mediterranean Sea Coast |
| title_sort | phlorotannins of the brown algae i sargassum vulgare i from the mediterranean sea coast |
| topic | <i>Sargassum</i> antioxidant antidiabetic pancreatic lipase anti-inflammatory UHPLC-MS analysis |
| url | https://www.mdpi.com/2076-3921/11/6/1055 |
| work_keys_str_mv | AT aminachouh phlorotanninsofthebrownalgaeisargassumvulgareifromthemediterraneanseacoast AT taharnouadri phlorotanninsofthebrownalgaeisargassumvulgareifromthemediterraneanseacoast AT marcelodcatarino phlorotanninsofthebrownalgaeisargassumvulgareifromthemediterraneanseacoast AT arturmssilva phlorotanninsofthebrownalgaeisargassumvulgareifromthemediterraneanseacoast AT susanamcardoso phlorotanninsofthebrownalgaeisargassumvulgareifromthemediterraneanseacoast |