Entorhinal Layer II Calbindin-Expressing Neurons Originate Widespread Telencephalic and Intrinsic Projections
In the present study we provide the first systematic and quantitative hodological study of the calbindin-expressing (CB+) principal neurons in layer II of the entorhinal cortex and compared the respective projections of the lateral and medial subdivisions of the entorhinal cortex. Using elaborate qu...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Systems Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnsys.2019.00054/full |
| _version_ | 1819170258982797312 |
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| author | Shinya Ohara Shinya Ohara Michele Gianatti Kazuki Itou Christin H. Berndtsson Thanh P. Doan Takuma Kitanishi Kenji Mizuseki Toshio Iijima Ken-Ichiro Tsutsui Menno P. Witter |
| author_facet | Shinya Ohara Shinya Ohara Michele Gianatti Kazuki Itou Christin H. Berndtsson Thanh P. Doan Takuma Kitanishi Kenji Mizuseki Toshio Iijima Ken-Ichiro Tsutsui Menno P. Witter |
| author_sort | Shinya Ohara |
| collection | DOAJ |
| description | In the present study we provide the first systematic and quantitative hodological study of the calbindin-expressing (CB+) principal neurons in layer II of the entorhinal cortex and compared the respective projections of the lateral and medial subdivisions of the entorhinal cortex. Using elaborate quantitative retrograde tracing, complemented by anterograde tracing, we report that the layer II CB+ population comprises neurons with diverse, mainly excitatory projections. At least half of them originate local intrinsic and commissural projections which distribute mainly to layer I and II. We further show that long-range CB+ projections from the two entorhinal subdivisions differ substantially in that MEC projections mainly target field CA1 of the hippocampus, whereas LEC CB+ projections distribute much more widely to a substantial number of known forebrain targets. This connectional difference between the CB+ populations in LEC and MEC is reminiscent of the overall projection pattern of the two entorhinal subdivisions. |
| first_indexed | 2024-12-22T19:32:33Z |
| format | Article |
| id | doaj.art-7d09edaf9f204fd38226e6dde0a2f6ee |
| institution | Directory Open Access Journal |
| issn | 1662-5137 |
| language | English |
| last_indexed | 2024-12-22T19:32:33Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Systems Neuroscience |
| spelling | doaj.art-7d09edaf9f204fd38226e6dde0a2f6ee2022-12-21T18:15:04ZengFrontiers Media S.A.Frontiers in Systems Neuroscience1662-51372019-10-011310.3389/fnsys.2019.00054474040Entorhinal Layer II Calbindin-Expressing Neurons Originate Widespread Telencephalic and Intrinsic ProjectionsShinya Ohara0Shinya Ohara1Michele Gianatti2Kazuki Itou3Christin H. Berndtsson4Thanh P. Doan5Takuma Kitanishi6Kenji Mizuseki7Toshio Iijima8Ken-Ichiro Tsutsui9Menno P. Witter10Kavli Institute for Systems Neuroscience, Center for Computational Neuroscience, Egil and Pauline Braathen and Fred Kavli Centre for Cortical Microcircuits, Norwegian University of Science and Technology (NTNU), Trondheim, NorwayLaboratory of Systems Neuroscience, Graduate School of Life Sciences, Tohoku University, Sendai, JapanKavli Institute for Systems Neuroscience, Center for Computational Neuroscience, Egil and Pauline Braathen and Fred Kavli Centre for Cortical Microcircuits, Norwegian University of Science and Technology (NTNU), Trondheim, NorwayLaboratory of Systems Neuroscience, Graduate School of Life Sciences, Tohoku University, Sendai, JapanKavli Institute for Systems Neuroscience, Center for Computational Neuroscience, Egil and Pauline Braathen and Fred Kavli Centre for Cortical Microcircuits, Norwegian University of Science and Technology (NTNU), Trondheim, NorwayKavli Institute for Systems Neuroscience, Center for Computational Neuroscience, Egil and Pauline Braathen and Fred Kavli Centre for Cortical Microcircuits, Norwegian University of Science and Technology (NTNU), Trondheim, NorwayDepartment of Physiology, Graduate School of Medicine, Osaka City University, Osaka, JapanDepartment of Physiology, Graduate School of Medicine, Osaka City University, Osaka, JapanLaboratory of Systems Neuroscience, Graduate School of Life Sciences, Tohoku University, Sendai, JapanLaboratory of Systems Neuroscience, Graduate School of Life Sciences, Tohoku University, Sendai, JapanKavli Institute for Systems Neuroscience, Center for Computational Neuroscience, Egil and Pauline Braathen and Fred Kavli Centre for Cortical Microcircuits, Norwegian University of Science and Technology (NTNU), Trondheim, NorwayIn the present study we provide the first systematic and quantitative hodological study of the calbindin-expressing (CB+) principal neurons in layer II of the entorhinal cortex and compared the respective projections of the lateral and medial subdivisions of the entorhinal cortex. Using elaborate quantitative retrograde tracing, complemented by anterograde tracing, we report that the layer II CB+ population comprises neurons with diverse, mainly excitatory projections. At least half of them originate local intrinsic and commissural projections which distribute mainly to layer I and II. We further show that long-range CB+ projections from the two entorhinal subdivisions differ substantially in that MEC projections mainly target field CA1 of the hippocampus, whereas LEC CB+ projections distribute much more widely to a substantial number of known forebrain targets. This connectional difference between the CB+ populations in LEC and MEC is reminiscent of the overall projection pattern of the two entorhinal subdivisions.https://www.frontiersin.org/article/10.3389/fnsys.2019.00054/fullmedial entorhinal cortexlateral entorhinal cortexparahippocampusconnectivityrodentcommissural projections |
| spellingShingle | Shinya Ohara Shinya Ohara Michele Gianatti Kazuki Itou Christin H. Berndtsson Thanh P. Doan Takuma Kitanishi Kenji Mizuseki Toshio Iijima Ken-Ichiro Tsutsui Menno P. Witter Entorhinal Layer II Calbindin-Expressing Neurons Originate Widespread Telencephalic and Intrinsic Projections Frontiers in Systems Neuroscience medial entorhinal cortex lateral entorhinal cortex parahippocampus connectivity rodent commissural projections |
| title | Entorhinal Layer II Calbindin-Expressing Neurons Originate Widespread Telencephalic and Intrinsic Projections |
| title_full | Entorhinal Layer II Calbindin-Expressing Neurons Originate Widespread Telencephalic and Intrinsic Projections |
| title_fullStr | Entorhinal Layer II Calbindin-Expressing Neurons Originate Widespread Telencephalic and Intrinsic Projections |
| title_full_unstemmed | Entorhinal Layer II Calbindin-Expressing Neurons Originate Widespread Telencephalic and Intrinsic Projections |
| title_short | Entorhinal Layer II Calbindin-Expressing Neurons Originate Widespread Telencephalic and Intrinsic Projections |
| title_sort | entorhinal layer ii calbindin expressing neurons originate widespread telencephalic and intrinsic projections |
| topic | medial entorhinal cortex lateral entorhinal cortex parahippocampus connectivity rodent commissural projections |
| url | https://www.frontiersin.org/article/10.3389/fnsys.2019.00054/full |
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