A novel toxoflavin‐quenching regulation in bacteria and its application to resistance cultivars
Summary The toxoflavin (Txn), broad host range phytotoxin produced by a variety of bacteria, including Burkholderia glumae, is a key pathogenicity factor of B. glumae in rice and field crops. Two bacteria exhibiting Txn‐degrading activity were isolated from healthy rice seeds and identified as Sphin...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-07-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13831 |
| _version_ | 1818610965305884672 |
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| author | Okhee Choi Yeyeong Lee Jiyeong Park Byeongsam Kang Hyun Jin Chun Min Chul Kim Jinwoo Kim |
| author_facet | Okhee Choi Yeyeong Lee Jiyeong Park Byeongsam Kang Hyun Jin Chun Min Chul Kim Jinwoo Kim |
| author_sort | Okhee Choi |
| collection | DOAJ |
| description | Summary The toxoflavin (Txn), broad host range phytotoxin produced by a variety of bacteria, including Burkholderia glumae, is a key pathogenicity factor of B. glumae in rice and field crops. Two bacteria exhibiting Txn‐degrading activity were isolated from healthy rice seeds and identified as Sphingomonas adhaesiva and Agrobacterium sp. respectively. The genes stdR and stdA, encoding proteins responsible for Txn degradation of both bacterial isolates, were identical, indicating that horizontal gene transfer occurred between microbial communities in the same ecosystem. We identified a novel Txn‐quenching regulation of bacteria, demonstrating that the LysR‐type transcriptional regulator (LTTR) StdR induces the expression of the stdA, which encodes a Txn‐degrading enzyme, in the presence of Txn as a coinducer. Here we show that the bacterial StdRTxn‐quenching regulatory system mimics the ToxRTxn‐mediated biosynthetic regulation of B. glumae. Substrate specificity investigations revealed that Txn is the only coinducer of StdR and that StdA has a high degree of specificity for Txn. Rice plants expressing StdA showed Txn resistance. Collectively, bacteria mimic the mechanism of Txn biosynthesis regulation, employ it in the development of a Txn‐quenching regulatory system and share it with neighbouring bacteria for survival in rice environments full of Txn. |
| first_indexed | 2024-12-16T15:22:49Z |
| format | Article |
| id | doaj.art-7d0b9e423b7547a380bc86d712778680 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-12-16T15:22:49Z |
| publishDate | 2021-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-7d0b9e423b7547a380bc86d7127786802022-12-21T22:26:35ZengWileyMicrobial Biotechnology1751-79152021-07-011441657167010.1111/1751-7915.13831A novel toxoflavin‐quenching regulation in bacteria and its application to resistance cultivarsOkhee Choi0Yeyeong Lee1Jiyeong Park2Byeongsam Kang3Hyun Jin Chun4Min Chul Kim5Jinwoo Kim6Institute of Agriculture and Life Science Gyeongsang National University Jinju52828South KoreaDepartment of Plant Medicine Gyeongsang National University Jinju South KoreaDepartment of Plant Medicine Gyeongsang National University Jinju South KoreaDivision of Applied Life Science Gyeongsang National University Jinju South KoreaInstitute of Agriculture and Life Science Gyeongsang National University Jinju52828South KoreaInstitute of Agriculture and Life Science Gyeongsang National University Jinju52828South KoreaInstitute of Agriculture and Life Science Gyeongsang National University Jinju52828South KoreaSummary The toxoflavin (Txn), broad host range phytotoxin produced by a variety of bacteria, including Burkholderia glumae, is a key pathogenicity factor of B. glumae in rice and field crops. Two bacteria exhibiting Txn‐degrading activity were isolated from healthy rice seeds and identified as Sphingomonas adhaesiva and Agrobacterium sp. respectively. The genes stdR and stdA, encoding proteins responsible for Txn degradation of both bacterial isolates, were identical, indicating that horizontal gene transfer occurred between microbial communities in the same ecosystem. We identified a novel Txn‐quenching regulation of bacteria, demonstrating that the LysR‐type transcriptional regulator (LTTR) StdR induces the expression of the stdA, which encodes a Txn‐degrading enzyme, in the presence of Txn as a coinducer. Here we show that the bacterial StdRTxn‐quenching regulatory system mimics the ToxRTxn‐mediated biosynthetic regulation of B. glumae. Substrate specificity investigations revealed that Txn is the only coinducer of StdR and that StdA has a high degree of specificity for Txn. Rice plants expressing StdA showed Txn resistance. Collectively, bacteria mimic the mechanism of Txn biosynthesis regulation, employ it in the development of a Txn‐quenching regulatory system and share it with neighbouring bacteria for survival in rice environments full of Txn.https://doi.org/10.1111/1751-7915.13831 |
| spellingShingle | Okhee Choi Yeyeong Lee Jiyeong Park Byeongsam Kang Hyun Jin Chun Min Chul Kim Jinwoo Kim A novel toxoflavin‐quenching regulation in bacteria and its application to resistance cultivars Microbial Biotechnology |
| title | A novel toxoflavin‐quenching regulation in bacteria and its application to resistance cultivars |
| title_full | A novel toxoflavin‐quenching regulation in bacteria and its application to resistance cultivars |
| title_fullStr | A novel toxoflavin‐quenching regulation in bacteria and its application to resistance cultivars |
| title_full_unstemmed | A novel toxoflavin‐quenching regulation in bacteria and its application to resistance cultivars |
| title_short | A novel toxoflavin‐quenching regulation in bacteria and its application to resistance cultivars |
| title_sort | novel toxoflavin quenching regulation in bacteria and its application to resistance cultivars |
| url | https://doi.org/10.1111/1751-7915.13831 |
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