Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling
Abstract Background Spermatogenesis is a complex process characterized by the activation and/or repression of a number of genes in a spatio-temporal manner. Pubertal development in males starts with the onset of the first spermatogenesis and implies the division of primary spermatogonia and their su...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-017-3823-2 |
| _version_ | 1817978239195283456 |
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| author | Mercedes Blázquez Paula Medina Berta Crespo Ana Gómez Silvia Zanuy |
| author_facet | Mercedes Blázquez Paula Medina Berta Crespo Ana Gómez Silvia Zanuy |
| author_sort | Mercedes Blázquez |
| collection | DOAJ |
| description | Abstract Background Spermatogenesis is a complex process characterized by the activation and/or repression of a number of genes in a spatio-temporal manner. Pubertal development in males starts with the onset of the first spermatogenesis and implies the division of primary spermatogonia and their subsequent entry into meiosis. This study is aimed at the characterization of genes involved in the onset of puberty in European sea bass, and constitutes the first transcriptomic approach focused on meiosis in this species. Results European sea bass testes collected at the onset of puberty (first successful reproduction) were grouped in stage I (resting stage), and stage II (proliferative stage). Transition from stage I to stage II was marked by an increase of 11ketotestosterone (11KT), the main fish androgen, whereas the transcriptomic study resulted in 315 genes differentially expressed between the two stages. The onset of puberty induced 1) an up-regulation of genes involved in cell proliferation, cell cycle and meiosis progression, 2) changes in genes related with reproduction and growth, and 3) a down-regulation of genes included in the retinoic acid (RA) signalling pathway. The analysis of GO-terms and biological pathways showed that cell cycle, cell division, cellular metabolic processes, and reproduction were affected, consistent with the early events that occur during the onset of puberty. Furthermore, changes in the expression of three RA nuclear receptors point at the importance of the RA-signalling pathway during this period, in agreement with its role in meiosis. Conclusion The results contribute to boost our knowledge of the early molecular and endocrine events that trigger pubertal development and the onset of spermatogenesis in fish. These include an increase in 11KT plasma levels and changes in the expression of several genes involved in cell proliferation, cell cycle progression, meiosis or RA-signalling pathway. Moreover, the results can be applied to study meiosis in this economically important fish species for Mediterranean countries, and may help to develop tools for its sustainable aquaculture. |
| first_indexed | 2024-04-13T22:26:50Z |
| format | Article |
| id | doaj.art-7d0bbe6854e44a9db08bea569a994980 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T22:26:50Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-7d0bbe6854e44a9db08bea569a9949802022-12-22T02:27:02ZengBMCBMC Genomics1471-21642017-06-0118111710.1186/s12864-017-3823-2Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profilingMercedes Blázquez0Paula Medina1Berta Crespo2Ana Gómez3Silvia Zanuy4Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones Científicas (IATS-CSIC)Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones Científicas (IATS-CSIC)Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones Científicas (IATS-CSIC)Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones Científicas (IATS-CSIC)Instituto de Acuicultura de Torre la Sal, Consejo Superior de Investigaciones Científicas (IATS-CSIC)Abstract Background Spermatogenesis is a complex process characterized by the activation and/or repression of a number of genes in a spatio-temporal manner. Pubertal development in males starts with the onset of the first spermatogenesis and implies the division of primary spermatogonia and their subsequent entry into meiosis. This study is aimed at the characterization of genes involved in the onset of puberty in European sea bass, and constitutes the first transcriptomic approach focused on meiosis in this species. Results European sea bass testes collected at the onset of puberty (first successful reproduction) were grouped in stage I (resting stage), and stage II (proliferative stage). Transition from stage I to stage II was marked by an increase of 11ketotestosterone (11KT), the main fish androgen, whereas the transcriptomic study resulted in 315 genes differentially expressed between the two stages. The onset of puberty induced 1) an up-regulation of genes involved in cell proliferation, cell cycle and meiosis progression, 2) changes in genes related with reproduction and growth, and 3) a down-regulation of genes included in the retinoic acid (RA) signalling pathway. The analysis of GO-terms and biological pathways showed that cell cycle, cell division, cellular metabolic processes, and reproduction were affected, consistent with the early events that occur during the onset of puberty. Furthermore, changes in the expression of three RA nuclear receptors point at the importance of the RA-signalling pathway during this period, in agreement with its role in meiosis. Conclusion The results contribute to boost our knowledge of the early molecular and endocrine events that trigger pubertal development and the onset of spermatogenesis in fish. These include an increase in 11KT plasma levels and changes in the expression of several genes involved in cell proliferation, cell cycle progression, meiosis or RA-signalling pathway. Moreover, the results can be applied to study meiosis in this economically important fish species for Mediterranean countries, and may help to develop tools for its sustainable aquaculture.http://link.springer.com/article/10.1186/s12864-017-3823-2SpermatogenesisCell cycleMeiosisRetinoic acidTeleosts |
| spellingShingle | Mercedes Blázquez Paula Medina Berta Crespo Ana Gómez Silvia Zanuy Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling BMC Genomics Spermatogenesis Cell cycle Meiosis Retinoic acid Teleosts |
| title | Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling |
| title_full | Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling |
| title_fullStr | Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling |
| title_full_unstemmed | Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling |
| title_short | Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling |
| title_sort | identification of conserved genes triggering puberty in european sea bass males dicentrarchus labrax by microarray expression profiling |
| topic | Spermatogenesis Cell cycle Meiosis Retinoic acid Teleosts |
| url | http://link.springer.com/article/10.1186/s12864-017-3823-2 |
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