Towards a Standardized Antimicrobial Susceptibility Testing Method for <i>Mycoplasma hyorhinis</i>
Conducting antimicrobial susceptibility testing (AST) in a comparable manner requires the availability of a standardized method. Organizations, such as the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST), provide standardi...
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/994 |
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| author | Lisa Käbisch Anne-Kathrin Schink Doris Höltig Joachim Spergser Corinna Kehrenberg Stefan Schwarz |
| author_facet | Lisa Käbisch Anne-Kathrin Schink Doris Höltig Joachim Spergser Corinna Kehrenberg Stefan Schwarz |
| author_sort | Lisa Käbisch |
| collection | DOAJ |
| description | Conducting antimicrobial susceptibility testing (AST) in a comparable manner requires the availability of a standardized method. Organizations, such as the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST), provide standardized protocols for a range of fastidious bacteria but not for <i>Mycoplasma hyorhinis</i>. We developed a broth microdilution method for testing <i>M. hyorhinis</i> in a standardized and harmonized way using a modified Friis broth devoid of antimicrobial or otherwise bacterial growth-inhibiting agents. The type strain <i>M. hyorhinis</i> DSM 25591 was chosen to establish the methodology. The antimicrobial agents of interest were doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin, tested by using commercial Sensititre<sup>TM</sup> microtiter plates. In addition, the suitability of the methodology was evaluated via variation of the individual ingredients of the modified Friis broth by either using different batches or choosing other distributors. Despite these alterations, the method provided reliable results. We obtained repeatable minimal inhibitory concentrations for all six tested field isolates and the <i>M. hyorhinis</i> type strain. With this newly proposed method, we aim to provide an improved AST method for diagnostic laboratories and monitoring purposes with better comparability between times and countries. In addition, this new method will allow for an improvement of targeted treatments using antimicrobial agents and thereby reduce the options for resistance development. |
| first_indexed | 2024-03-11T04:43:38Z |
| format | Article |
| id | doaj.art-7d107314b5934c87811bfd8c6060767c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:43:38Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7d107314b5934c87811bfd8c6060767c2023-11-17T20:33:29ZengMDPI AGMicroorganisms2076-26072023-04-0111499410.3390/microorganisms11040994Towards a Standardized Antimicrobial Susceptibility Testing Method for <i>Mycoplasma hyorhinis</i>Lisa Käbisch0Anne-Kathrin Schink1Doris Höltig2Joachim Spergser3Corinna Kehrenberg4Stefan Schwarz5Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, GermanyInstitute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, GermanyVeterinary Centre for Resistance Research (TZR), Freie Universität Berlin, 14163 Berlin, GermanyInstitute of Microbiology, University of Veterinary Medicine, 1210 Vienna, AustriaInstitute for Veterinary Food Science, Department of Veterinary Medicine, Justus-Liebig-University Gießen, 35392 Gießen, GermanyInstitute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, GermanyConducting antimicrobial susceptibility testing (AST) in a comparable manner requires the availability of a standardized method. Organizations, such as the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST), provide standardized protocols for a range of fastidious bacteria but not for <i>Mycoplasma hyorhinis</i>. We developed a broth microdilution method for testing <i>M. hyorhinis</i> in a standardized and harmonized way using a modified Friis broth devoid of antimicrobial or otherwise bacterial growth-inhibiting agents. The type strain <i>M. hyorhinis</i> DSM 25591 was chosen to establish the methodology. The antimicrobial agents of interest were doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin, tested by using commercial Sensititre<sup>TM</sup> microtiter plates. In addition, the suitability of the methodology was evaluated via variation of the individual ingredients of the modified Friis broth by either using different batches or choosing other distributors. Despite these alterations, the method provided reliable results. We obtained repeatable minimal inhibitory concentrations for all six tested field isolates and the <i>M. hyorhinis</i> type strain. With this newly proposed method, we aim to provide an improved AST method for diagnostic laboratories and monitoring purposes with better comparability between times and countries. In addition, this new method will allow for an improvement of targeted treatments using antimicrobial agents and thereby reduce the options for resistance development.https://www.mdpi.com/2076-2607/11/4/994antimicrobial susceptibility testing (AST)broth microdilutionstandardized methodology<i>Mycoplasma hyorhinis</i> |
| spellingShingle | Lisa Käbisch Anne-Kathrin Schink Doris Höltig Joachim Spergser Corinna Kehrenberg Stefan Schwarz Towards a Standardized Antimicrobial Susceptibility Testing Method for <i>Mycoplasma hyorhinis</i> Microorganisms antimicrobial susceptibility testing (AST) broth microdilution standardized methodology <i>Mycoplasma hyorhinis</i> |
| title | Towards a Standardized Antimicrobial Susceptibility Testing Method for <i>Mycoplasma hyorhinis</i> |
| title_full | Towards a Standardized Antimicrobial Susceptibility Testing Method for <i>Mycoplasma hyorhinis</i> |
| title_fullStr | Towards a Standardized Antimicrobial Susceptibility Testing Method for <i>Mycoplasma hyorhinis</i> |
| title_full_unstemmed | Towards a Standardized Antimicrobial Susceptibility Testing Method for <i>Mycoplasma hyorhinis</i> |
| title_short | Towards a Standardized Antimicrobial Susceptibility Testing Method for <i>Mycoplasma hyorhinis</i> |
| title_sort | towards a standardized antimicrobial susceptibility testing method for i mycoplasma hyorhinis i |
| topic | antimicrobial susceptibility testing (AST) broth microdilution standardized methodology <i>Mycoplasma hyorhinis</i> |
| url | https://www.mdpi.com/2076-2607/11/4/994 |
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