Effects of Enterococcus faecium (R8a) on nonspecific immune gene expression, immunity and intestinal flora of giant tiger shrimp (Penaeus monodon)
Abstract In this study, Penaeus monodon were gave basic feed supplemented with three levels of Enterococcus faecium. Then, the expression of non-specific immunity-related genes, and the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), malondialdehyde (MDA), acid phosphat...
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| Format: | Artykuł |
| Język: | English |
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Nature Portfolio
2024-01-01
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| Seria: | Scientific Reports |
| Dostęp online: | https://doi.org/10.1038/s41598-024-52496-4 |
| _version_ | 1827377328660938752 |
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| author | Xueliang Sun Zhenzhen Fang Hong Yu Honghao Zhao Yang Wang Falin Zhou Lin Zhao Jingfeng Sun Yunchen Tian |
| author_facet | Xueliang Sun Zhenzhen Fang Hong Yu Honghao Zhao Yang Wang Falin Zhou Lin Zhao Jingfeng Sun Yunchen Tian |
| author_sort | Xueliang Sun |
| collection | DOAJ |
| description | Abstract In this study, Penaeus monodon were gave basic feed supplemented with three levels of Enterococcus faecium. Then, the expression of non-specific immunity-related genes, and the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), malondialdehyde (MDA), acid phosphatase (ACP), alkaline phosphatase (AKP), phenol oxidase (PO) were evaluated. Meanwhile, the disease resistance test and intestinal flora determination were conducted. The results showed that the MDA levels of 2% and 5% E. faecium groups were significantly lower than that of the control group (P < 0.05). While the SOD and T-AOC and ACP and AKP of experimental groups were significantly higher (P < 0.05), the PO of experimental groups were significantly lower than that of the control group (P < 0.05). In addition, the expressions of immunity-related genes (tlr22, dorsal, lysozyme, crustin, imd, and relish) in the 2% and 5% E. faecalis groups were significantly greater than those in the control group (P < 0.05). After P. monodon was challenged with Vibrio parahaemolyticus for 7 days, the average cumulative mortality of P. monodon in the 2% and 5% groups were significantly lower than that in the 0% group (P < 0.05). With the increase of feeding time, the number of effective OTUs in each group showed a downward trend. At the 14th d, Proteobacteria, Bacteroidetes and Firmicutes, the dominant flora in the intestinal tract of P. monodon. In summary, supplied with E. faecium could increase the expression of non-specific immunity-related genes, enhance the immune capacity of P. monodon. |
| first_indexed | 2024-03-08T12:37:33Z |
| format | Article |
| id | doaj.art-7d13cecfd51942e7adace4dd1d0696b5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T12:37:33Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7d13cecfd51942e7adace4dd1d0696b52024-01-21T12:22:26ZengNature PortfolioScientific Reports2045-23222024-01-0114111510.1038/s41598-024-52496-4Effects of Enterococcus faecium (R8a) on nonspecific immune gene expression, immunity and intestinal flora of giant tiger shrimp (Penaeus monodon)Xueliang Sun0Zhenzhen Fang1Hong Yu2Honghao Zhao3Yang Wang4Falin Zhou5Lin Zhao6Jingfeng Sun7Yunchen Tian8Tianjin University, Environment CollegeTianjin Agricultural UniversityTianjin Agricultural UniversityTianjin Agricultural UniversityTianjin Agricultural UniversityInstitute of South China Sea Oceanography, Chinese Academy of SciencesTianjin University, Environment CollegeTianjin Agricultural UniversityTianjin Agricultural UniversityAbstract In this study, Penaeus monodon were gave basic feed supplemented with three levels of Enterococcus faecium. Then, the expression of non-specific immunity-related genes, and the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), malondialdehyde (MDA), acid phosphatase (ACP), alkaline phosphatase (AKP), phenol oxidase (PO) were evaluated. Meanwhile, the disease resistance test and intestinal flora determination were conducted. The results showed that the MDA levels of 2% and 5% E. faecium groups were significantly lower than that of the control group (P < 0.05). While the SOD and T-AOC and ACP and AKP of experimental groups were significantly higher (P < 0.05), the PO of experimental groups were significantly lower than that of the control group (P < 0.05). In addition, the expressions of immunity-related genes (tlr22, dorsal, lysozyme, crustin, imd, and relish) in the 2% and 5% E. faecalis groups were significantly greater than those in the control group (P < 0.05). After P. monodon was challenged with Vibrio parahaemolyticus for 7 days, the average cumulative mortality of P. monodon in the 2% and 5% groups were significantly lower than that in the 0% group (P < 0.05). With the increase of feeding time, the number of effective OTUs in each group showed a downward trend. At the 14th d, Proteobacteria, Bacteroidetes and Firmicutes, the dominant flora in the intestinal tract of P. monodon. In summary, supplied with E. faecium could increase the expression of non-specific immunity-related genes, enhance the immune capacity of P. monodon.https://doi.org/10.1038/s41598-024-52496-4 |
| spellingShingle | Xueliang Sun Zhenzhen Fang Hong Yu Honghao Zhao Yang Wang Falin Zhou Lin Zhao Jingfeng Sun Yunchen Tian Effects of Enterococcus faecium (R8a) on nonspecific immune gene expression, immunity and intestinal flora of giant tiger shrimp (Penaeus monodon) Scientific Reports |
| title | Effects of Enterococcus faecium (R8a) on nonspecific immune gene expression, immunity and intestinal flora of giant tiger shrimp (Penaeus monodon) |
| title_full | Effects of Enterococcus faecium (R8a) on nonspecific immune gene expression, immunity and intestinal flora of giant tiger shrimp (Penaeus monodon) |
| title_fullStr | Effects of Enterococcus faecium (R8a) on nonspecific immune gene expression, immunity and intestinal flora of giant tiger shrimp (Penaeus monodon) |
| title_full_unstemmed | Effects of Enterococcus faecium (R8a) on nonspecific immune gene expression, immunity and intestinal flora of giant tiger shrimp (Penaeus monodon) |
| title_short | Effects of Enterococcus faecium (R8a) on nonspecific immune gene expression, immunity and intestinal flora of giant tiger shrimp (Penaeus monodon) |
| title_sort | effects of enterococcus faecium r8a on nonspecific immune gene expression immunity and intestinal flora of giant tiger shrimp penaeus monodon |
| url | https://doi.org/10.1038/s41598-024-52496-4 |
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