Innovative Green Approach for Extraction of Piperine from Black Pepper Based on Response Surface Methodology
Bioactive compounds like piperine (alkaloids) offer a variety of health benefits due to their biological and pharmacological potential. Piperine has been revealed to have anti-inflammatory, anti-aging, anti-diabetes, anti-bacterial, anti-ulcer, and anti-carcinogenic characteristics. Recent research...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Sustainable Chemistry |
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| Online Access: | https://www.mdpi.com/2673-4079/4/1/5 |
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| author | Charles Lwamba Saied A. Aboushanab Ranga Rao Ambati Elena G. Kovaleva |
| author_facet | Charles Lwamba Saied A. Aboushanab Ranga Rao Ambati Elena G. Kovaleva |
| author_sort | Charles Lwamba |
| collection | DOAJ |
| description | Bioactive compounds like piperine (alkaloids) offer a variety of health benefits due to their biological and pharmacological potential. Piperine has been revealed to have anti-inflammatory, anti-aging, anti-diabetes, anti-bacterial, anti-ulcer, and anti-carcinogenic characteristics. Recent research has been conducted to extract piperine using effective and environmentally friendly techniques. In this study, we sought to assess the potential and efficacy of natural deep eutectic solvents to extract piperine from black pepper seeds using an ultrasound-assisted extraction technique. A Box–Behnken design combined with response surface methodology was used to evaluate the optimum extraction conditions of piperine. Extraction efficiency was evaluated based on the extraction yields of piperine, antioxidant activity, total polyphenols, and total flavonoids. The results showed that the choline chloride-citric acid-1,2-propylene glycol combination (1:2:2 molar ratio) with 25% (<i>v</i>/<i>v</i>) of water was the most effective at extracting piperine from black pepper. It was found that the extraction yield of piperine was significantly influenced by the liquid–solid ratio and extraction time. The optimal extraction conditions were determined and it was found that antioxidant activities and total polyphenol content in the piperine-rich extracts were remarkably related to the piperine content. The piperine extract purity was found to be 90%. Our results indicate that black pepper could be used as a functional food application. |
| first_indexed | 2024-03-11T05:54:26Z |
| format | Article |
| id | doaj.art-7d15e823cfe545649752f91f49178523 |
| institution | Directory Open Access Journal |
| issn | 2673-4079 |
| language | English |
| last_indexed | 2024-03-11T05:54:26Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sustainable Chemistry |
| spelling | doaj.art-7d15e823cfe545649752f91f491785232023-11-17T13:54:50ZengMDPI AGSustainable Chemistry2673-40792023-01-0141405310.3390/suschem4010005Innovative Green Approach for Extraction of Piperine from Black Pepper Based on Response Surface MethodologyCharles Lwamba0Saied A. Aboushanab1Ranga Rao Ambati2Elena G. Kovaleva3Institute of Chemical Engineering, Ural Federal University Named after the First President of Russia B. N. Yeltsin, Mira 19, Yekaterinburg 620002, RussiaInstitute of Chemical Engineering, Ural Federal University Named after the First President of Russia B. N. Yeltsin, Mira 19, Yekaterinburg 620002, RussiaDepartment of Biotechnology, School of Biotechnology and Pharmaceutical Sciences, Vignan’s Foundation of Science, Technology and Research (Deemed to Be University), Vadlamudi, Guntur 522213, Andhra Pradesh, IndiaInstitute of Chemical Engineering, Ural Federal University Named after the First President of Russia B. N. Yeltsin, Mira 19, Yekaterinburg 620002, RussiaBioactive compounds like piperine (alkaloids) offer a variety of health benefits due to their biological and pharmacological potential. Piperine has been revealed to have anti-inflammatory, anti-aging, anti-diabetes, anti-bacterial, anti-ulcer, and anti-carcinogenic characteristics. Recent research has been conducted to extract piperine using effective and environmentally friendly techniques. In this study, we sought to assess the potential and efficacy of natural deep eutectic solvents to extract piperine from black pepper seeds using an ultrasound-assisted extraction technique. A Box–Behnken design combined with response surface methodology was used to evaluate the optimum extraction conditions of piperine. Extraction efficiency was evaluated based on the extraction yields of piperine, antioxidant activity, total polyphenols, and total flavonoids. The results showed that the choline chloride-citric acid-1,2-propylene glycol combination (1:2:2 molar ratio) with 25% (<i>v</i>/<i>v</i>) of water was the most effective at extracting piperine from black pepper. It was found that the extraction yield of piperine was significantly influenced by the liquid–solid ratio and extraction time. The optimal extraction conditions were determined and it was found that antioxidant activities and total polyphenol content in the piperine-rich extracts were remarkably related to the piperine content. The piperine extract purity was found to be 90%. Our results indicate that black pepper could be used as a functional food application.https://www.mdpi.com/2673-4079/4/1/5<i>Piper nigrum</i> L.piperineeutectic solventflavonoidspolyphenolsresponse surface methodology |
| spellingShingle | Charles Lwamba Saied A. Aboushanab Ranga Rao Ambati Elena G. Kovaleva Innovative Green Approach for Extraction of Piperine from Black Pepper Based on Response Surface Methodology Sustainable Chemistry <i>Piper nigrum</i> L. piperine eutectic solvent flavonoids polyphenols response surface methodology |
| title | Innovative Green Approach for Extraction of Piperine from Black Pepper Based on Response Surface Methodology |
| title_full | Innovative Green Approach for Extraction of Piperine from Black Pepper Based on Response Surface Methodology |
| title_fullStr | Innovative Green Approach for Extraction of Piperine from Black Pepper Based on Response Surface Methodology |
| title_full_unstemmed | Innovative Green Approach for Extraction of Piperine from Black Pepper Based on Response Surface Methodology |
| title_short | Innovative Green Approach for Extraction of Piperine from Black Pepper Based on Response Surface Methodology |
| title_sort | innovative green approach for extraction of piperine from black pepper based on response surface methodology |
| topic | <i>Piper nigrum</i> L. piperine eutectic solvent flavonoids polyphenols response surface methodology |
| url | https://www.mdpi.com/2673-4079/4/1/5 |
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