Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of <i>Pyrus communis</i>
This study aimed to investigate the presence of beneficial microorganisms in the micropropagation of <i>Pyrus communis</i> rootstocks through the isolation, identification, and selection of auxin-producing bacteria. The selected microorganisms were also inoculated in vitro to evaluate th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/5/1226 |
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| author | Michele Carla Nadal Gustavo Magno dos Reis Ferreira Gracielle Vidal Silva Andrade Victor Hugo Buttrós Filipe Almendagna Rodrigues Caroline Marcela da Silva Adalvan Daniel Martins Léo Rufato José Magno Queiroz Luz Joyce Dória Moacir Pasqual |
| author_facet | Michele Carla Nadal Gustavo Magno dos Reis Ferreira Gracielle Vidal Silva Andrade Victor Hugo Buttrós Filipe Almendagna Rodrigues Caroline Marcela da Silva Adalvan Daniel Martins Léo Rufato José Magno Queiroz Luz Joyce Dória Moacir Pasqual |
| author_sort | Michele Carla Nadal |
| collection | DOAJ |
| description | This study aimed to investigate the presence of beneficial microorganisms in the micropropagation of <i>Pyrus communis</i> rootstocks through the isolation, identification, and selection of auxin-producing bacteria. The selected microorganisms were also inoculated in vitro to evaluate their effect on the plant rooting process and their behavior during the acclimatization phase. The results showed the presence of endophytic microorganisms in plant tissue cultures of the ‘OH×F87′ and ‘PDW’ selections. A low diversity was observed in the population of microorganisms isolated from the in vitro culture of the ‘OH×F87’and ‘PDW’ selections, with a predominance of the genera <i>Acinetobacter</i>, <i>Bacillus</i>, and <i>Buttiauxella</i>. The selection of promoting microorganisms was performed based on the auxin production test, in which 30.36% of the microorganisms tested positive. In the in vitro inoculation, it was possible to observe the promotion of growth and emission of roots in the pear rootstocks, from the inoculation with bacteria identified as capable of producing auxin. This process can be used to replace the inclusion of synthetic auxin in the productive chain of woody fruit trees propagated by vegetative means, such as pears. None of the bacterial isolates was notably more promising, but the general similarity of treatments containing the <i>A. septicus</i> and <i>A. ursingii</i> strains, with the synthetic auxin treatment, suggests the possibility of its use on a large scale allowing the adoption of the cheaper method. of rooting. This work opens the door for further research using new, more promising microbial isolates, and also for lower-cost microorganism cultivation techniques, such as low-cost media obtained from agro-industrial residues. |
| first_indexed | 2024-03-10T03:29:21Z |
| format | Article |
| id | doaj.art-7d16186a29374f35b136b4ed9dece1e7 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T03:29:21Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-7d16186a29374f35b136b4ed9dece1e72023-11-23T09:44:49ZengMDPI AGAgronomy2073-43952022-05-01125122610.3390/agronomy12051226Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of <i>Pyrus communis</i>Michele Carla Nadal0Gustavo Magno dos Reis Ferreira1Gracielle Vidal Silva Andrade2Victor Hugo Buttrós3Filipe Almendagna Rodrigues4Caroline Marcela da Silva5Adalvan Daniel Martins6Léo Rufato7José Magno Queiroz Luz8Joyce Dória9Moacir Pasqual10Department of Agriculture, Federal University of Lavras, P.O. Box 3037, Lavras 37200-900, MG, BrazilDepartment of Agriculture, Federal University of Lavras, P.O. Box 3037, Lavras 37200-900, MG, BrazilDepartment of Agriculture, Federal University of Lavras, P.O. Box 3037, Lavras 37200-900, MG, BrazilDepartment of Agriculture, Federal University of Lavras, P.O. Box 3037, Lavras 37200-900, MG, BrazilDepartment of Agriculture, Federal University of Lavras, P.O. Box 3037, Lavras 37200-900, MG, BrazilDepartment of Biology, Federal University of Lavras, P.O. Box 3037, Lavras 37200-900, MG, BrazilDepartment of Agriculture, Federal University of Lavras, P.O. Box 3037, Lavras 37200-900, MG, BrazilAgro-Veterinary Center, Santa Catarina State University, Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages 88520-000, SC, BrazilInstitute of Agricultural Sciences, Federal University of Uberlândia, BR 050, km 78, Uberlândia 38410-337, MG, BrazilDepartment of Agriculture, Federal University of Lavras, P.O. Box 3037, Lavras 37200-900, MG, BrazilDepartment of Agriculture, Federal University of Lavras, P.O. Box 3037, Lavras 37200-900, MG, BrazilThis study aimed to investigate the presence of beneficial microorganisms in the micropropagation of <i>Pyrus communis</i> rootstocks through the isolation, identification, and selection of auxin-producing bacteria. The selected microorganisms were also inoculated in vitro to evaluate their effect on the plant rooting process and their behavior during the acclimatization phase. The results showed the presence of endophytic microorganisms in plant tissue cultures of the ‘OH×F87′ and ‘PDW’ selections. A low diversity was observed in the population of microorganisms isolated from the in vitro culture of the ‘OH×F87’and ‘PDW’ selections, with a predominance of the genera <i>Acinetobacter</i>, <i>Bacillus</i>, and <i>Buttiauxella</i>. The selection of promoting microorganisms was performed based on the auxin production test, in which 30.36% of the microorganisms tested positive. In the in vitro inoculation, it was possible to observe the promotion of growth and emission of roots in the pear rootstocks, from the inoculation with bacteria identified as capable of producing auxin. This process can be used to replace the inclusion of synthetic auxin in the productive chain of woody fruit trees propagated by vegetative means, such as pears. None of the bacterial isolates was notably more promising, but the general similarity of treatments containing the <i>A. septicus</i> and <i>A. ursingii</i> strains, with the synthetic auxin treatment, suggests the possibility of its use on a large scale allowing the adoption of the cheaper method. of rooting. This work opens the door for further research using new, more promising microbial isolates, and also for lower-cost microorganism cultivation techniques, such as low-cost media obtained from agro-industrial residues.https://www.mdpi.com/2073-4395/12/5/1226plant micropropagation techniquesbacteria identificationbacteria isolationplant growth-promoting microorganismsseedling production |
| spellingShingle | Michele Carla Nadal Gustavo Magno dos Reis Ferreira Gracielle Vidal Silva Andrade Victor Hugo Buttrós Filipe Almendagna Rodrigues Caroline Marcela da Silva Adalvan Daniel Martins Léo Rufato José Magno Queiroz Luz Joyce Dória Moacir Pasqual Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of <i>Pyrus communis</i> Agronomy plant micropropagation techniques bacteria identification bacteria isolation plant growth-promoting microorganisms seedling production |
| title | Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of <i>Pyrus communis</i> |
| title_full | Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of <i>Pyrus communis</i> |
| title_fullStr | Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of <i>Pyrus communis</i> |
| title_full_unstemmed | Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of <i>Pyrus communis</i> |
| title_short | Endophytic Bacteria Can Replace the Need for Synthetic Auxin during In Vitro Rooting of <i>Pyrus communis</i> |
| title_sort | endophytic bacteria can replace the need for synthetic auxin during in vitro rooting of i pyrus communis i |
| topic | plant micropropagation techniques bacteria identification bacteria isolation plant growth-promoting microorganisms seedling production |
| url | https://www.mdpi.com/2073-4395/12/5/1226 |
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