Serological Assays for Alveolar and Cystic Echinococcosis—A Comparative Multi-Test Study in Switzerland and Kyrgyzstan
Both alveolar (AE) and cystic echinococcosis (CE) are lacking pathognomonic clinical signs; consequently imaging technologies and serology remain the main pillars for diagnosis. The present study included 100 confirmed treatment-naïve AE and 64 CE patients that were diagnosed in Switzerland or Kyrgy...
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MDPI AG
2022-04-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/5/518 |
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| author | Philipp A. Kronenberg Ansgar Deibel Bruno Gottstein Felix Grimm Beat Müllhaupt Cordula Meyer zu Schwabedissen Sezdbek Aitbaev Rakhatbek A. Omorov Kubanychbek K. Abdykerimov Gulnara Minbaeva Jumagul Usubalieva Mar Siles-Lucas Paola Pepe Laura Rinaldi Markus Spiliotis Junhua Wang Norbert Müller Paul R. Torgerson Peter Deplazes |
| author_facet | Philipp A. Kronenberg Ansgar Deibel Bruno Gottstein Felix Grimm Beat Müllhaupt Cordula Meyer zu Schwabedissen Sezdbek Aitbaev Rakhatbek A. Omorov Kubanychbek K. Abdykerimov Gulnara Minbaeva Jumagul Usubalieva Mar Siles-Lucas Paola Pepe Laura Rinaldi Markus Spiliotis Junhua Wang Norbert Müller Paul R. Torgerson Peter Deplazes |
| author_sort | Philipp A. Kronenberg |
| collection | DOAJ |
| description | Both alveolar (AE) and cystic echinococcosis (CE) are lacking pathognomonic clinical signs; consequently imaging technologies and serology remain the main pillars for diagnosis. The present study included 100 confirmed treatment-naïve AE and 64 CE patients that were diagnosed in Switzerland or Kyrgyzstan. Overall, 10 native <i>Echinococcus</i> spp. antigens, 3 recombinant antigens, and 4 commercial assays were comparatively evaluated. All native <i>E. multilocularis</i> antigens were produced in duplicates with a European and a Kyrgyz isolate and showed identical test values for the diagnosis of AE and CE. Native antigens and three commercial tests showed high diagnostic sensitivities (Se: 86–96%) and specificities (Sp: 96–99%) for the diagnosis of AE and CE in Swiss patients. In Kyrgyz patients, values of sensitivities and specificities were 10–20% lower as compared to the Swiss patients’ findings. For the sero-diagnosis of AE in Kyrgyzstan, a test-combination of an <i>E. multilocularis</i> protoscolex antigen and the recombinant antigen Em95 appears to be the most suitable test strategy (Se: 98%, Sp: 87%). For the diagnosis of CE in both countries, test performances were hampered by major cross-reactions with AE patients and other parasitic diseases as well as by limited diagnostic sensitivities (93% in Switzerland and 76% in Kyrgyzstan, respectively). |
| first_indexed | 2024-03-10T03:12:26Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T03:12:26Z |
| publishDate | 2022-04-01 |
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| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-7d1824bc53dc4abc92ca9610d7bf1fd32023-11-23T12:31:54ZengMDPI AGPathogens2076-08172022-04-0111551810.3390/pathogens11050518Serological Assays for Alveolar and Cystic Echinococcosis—A Comparative Multi-Test Study in Switzerland and KyrgyzstanPhilipp A. Kronenberg0Ansgar Deibel1Bruno Gottstein2Felix Grimm3Beat Müllhaupt4Cordula Meyer zu Schwabedissen5Sezdbek Aitbaev6Rakhatbek A. Omorov7Kubanychbek K. Abdykerimov8Gulnara Minbaeva9Jumagul Usubalieva10Mar Siles-Lucas11Paola Pepe12Laura Rinaldi13Markus Spiliotis14Junhua Wang15Norbert Müller16Paul R. Torgerson17Peter Deplazes18Institute of Parasitology, Vetsuisse and Medical Faculty, University of Zurich, 8075 Zurich, SwitzerlandClinics of Hepatology and Gastroenterology, University Hospital Zurich, 8091 Zurich, SwitzerlandLaboratory of Parasitology, Institute for Infectious Diseases, Medical Faculty, University of Bern, 3001 Bern, SwitzerlandInstitute of Parasitology, Vetsuisse and Medical Faculty, University of Zurich, 8075 Zurich, SwitzerlandClinics of Hepatology and Gastroenterology, University Hospital Zurich, 8091 Zurich, SwitzerlandClinics of Hepatology and Gastroenterology, University Hospital Zurich, 8091 Zurich, SwitzerlandCity Clinical Hospital #1, Surgical Department, Faculty of Surgery of the Kyrgyz State Medical Academy, Bishkek 720054, KyrgyzstanCity Clinical Hospital #1, Surgical Department, Faculty of Surgery of the Kyrgyz State Medical Academy, Bishkek 720054, KyrgyzstanSection of Epidemiology, Vetsuisse Faculty, University of Zurich, 8057 Zurich, SwitzerlandGovernment Sanito-Epidemiology Unit, Kyrgyz Ministry of Health, Bishkek 720033, KyrgyzstanGovernment Sanito-Epidemiology Unit, Kyrgyz Ministry of Health, Bishkek 720033, KyrgyzstanInstituto de Recursos Naturales y Agrobiologia (IRNASA-CSIC), 37008 Salamanca, SpainUnit of Parasitology and Parasitic Diseases, Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80138 Naples, ItalyUnit of Parasitology and Parasitic Diseases, Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80138 Naples, ItalyLaboratory of Parasitology, Institute for Infectious Diseases, Medical Faculty, University of Bern, 3001 Bern, SwitzerlandLaboratory of Parasitology, Institute for Infectious Diseases, Medical Faculty, University of Bern, 3001 Bern, SwitzerlandInstitute of Parasitology, Vetsuisse Faculty, University of Bern, 3012 Bern, SwitzerlandSection of Epidemiology, Vetsuisse Faculty, University of Zurich, 8057 Zurich, SwitzerlandInstitute of Parasitology, Vetsuisse and Medical Faculty, University of Zurich, 8075 Zurich, SwitzerlandBoth alveolar (AE) and cystic echinococcosis (CE) are lacking pathognomonic clinical signs; consequently imaging technologies and serology remain the main pillars for diagnosis. The present study included 100 confirmed treatment-naïve AE and 64 CE patients that were diagnosed in Switzerland or Kyrgyzstan. Overall, 10 native <i>Echinococcus</i> spp. antigens, 3 recombinant antigens, and 4 commercial assays were comparatively evaluated. All native <i>E. multilocularis</i> antigens were produced in duplicates with a European and a Kyrgyz isolate and showed identical test values for the diagnosis of AE and CE. Native antigens and three commercial tests showed high diagnostic sensitivities (Se: 86–96%) and specificities (Sp: 96–99%) for the diagnosis of AE and CE in Swiss patients. In Kyrgyz patients, values of sensitivities and specificities were 10–20% lower as compared to the Swiss patients’ findings. For the sero-diagnosis of AE in Kyrgyzstan, a test-combination of an <i>E. multilocularis</i> protoscolex antigen and the recombinant antigen Em95 appears to be the most suitable test strategy (Se: 98%, Sp: 87%). For the diagnosis of CE in both countries, test performances were hampered by major cross-reactions with AE patients and other parasitic diseases as well as by limited diagnostic sensitivities (93% in Switzerland and 76% in Kyrgyzstan, respectively).https://www.mdpi.com/2076-0817/11/5/518<i>Echinococcus multilocularis</i><i>Echinococcus granulosus sensu lato</i>diagnosisserologyantigensantibodies |
| spellingShingle | Philipp A. Kronenberg Ansgar Deibel Bruno Gottstein Felix Grimm Beat Müllhaupt Cordula Meyer zu Schwabedissen Sezdbek Aitbaev Rakhatbek A. Omorov Kubanychbek K. Abdykerimov Gulnara Minbaeva Jumagul Usubalieva Mar Siles-Lucas Paola Pepe Laura Rinaldi Markus Spiliotis Junhua Wang Norbert Müller Paul R. Torgerson Peter Deplazes Serological Assays for Alveolar and Cystic Echinococcosis—A Comparative Multi-Test Study in Switzerland and Kyrgyzstan Pathogens <i>Echinococcus multilocularis</i> <i>Echinococcus granulosus sensu lato</i> diagnosis serology antigens antibodies |
| title | Serological Assays for Alveolar and Cystic Echinococcosis—A Comparative Multi-Test Study in Switzerland and Kyrgyzstan |
| title_full | Serological Assays for Alveolar and Cystic Echinococcosis—A Comparative Multi-Test Study in Switzerland and Kyrgyzstan |
| title_fullStr | Serological Assays for Alveolar and Cystic Echinococcosis—A Comparative Multi-Test Study in Switzerland and Kyrgyzstan |
| title_full_unstemmed | Serological Assays for Alveolar and Cystic Echinococcosis—A Comparative Multi-Test Study in Switzerland and Kyrgyzstan |
| title_short | Serological Assays for Alveolar and Cystic Echinococcosis—A Comparative Multi-Test Study in Switzerland and Kyrgyzstan |
| title_sort | serological assays for alveolar and cystic echinococcosis a comparative multi test study in switzerland and kyrgyzstan |
| topic | <i>Echinococcus multilocularis</i> <i>Echinococcus granulosus sensu lato</i> diagnosis serology antigens antibodies |
| url | https://www.mdpi.com/2076-0817/11/5/518 |
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