Rapid Identification of Pollen- and Anther-Specific Genes in Response to High-Temperature Stress Based on Transcriptome Profiling Analysis in Cotton
Anther indehiscence and pollen sterility caused by high temperature (HT) stress have become a major problem that decreases the yield of cotton. Pollen- and anther-specific genes play a critical role in the process of male reproduction and the response to HT stress. In order to identify pollen-specif...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/6/3378 |
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| author | Rui Zhang Lili Zhou Yanlong Li Huanhuan Ma Yawei Li Yizan Ma Rongjie Lv Jing Yang Weiran Wang Aierxi Alifu Xianlong Zhang Jie Kong Ling Min |
| author_facet | Rui Zhang Lili Zhou Yanlong Li Huanhuan Ma Yawei Li Yizan Ma Rongjie Lv Jing Yang Weiran Wang Aierxi Alifu Xianlong Zhang Jie Kong Ling Min |
| author_sort | Rui Zhang |
| collection | DOAJ |
| description | Anther indehiscence and pollen sterility caused by high temperature (HT) stress have become a major problem that decreases the yield of cotton. Pollen- and anther-specific genes play a critical role in the process of male reproduction and the response to HT stress. In order to identify pollen-specific genes that respond to HT stress, a comparative transcriptome profiling analysis was performed in the pollen and anthers of <i>Gossypium hirsutum</i> HT-sensitive Line H05 against other tissue types under normal temperature (NT) conditions, and the analysis of a differentially expressed gene was conducted in the pollen of H05 under NT and HT conditions. In total, we identified 1111 pollen-specific genes (PSGs), 1066 anther-specific genes (ASGs), and 833 pollen differentially expressed genes (DEGs). Moreover, we found that the late stage of anther included more anther- and pollen-specific genes (APSGs). Stress-related <i>cis</i>-regulatory elements (CREs) and hormone-responsive CREs are enriched in the promoters of APSGs, suggesting that APSGs may respond to HT stress. However, 833 pollen DEGs had only 10 common genes with 1111 PSGs, indicating that PSGs are mainly involved in the processes of pollen development and do not respond to HT stress. Promoters of these 10 common genes are enriched for stress-related CREs and MeJA-responsive CREs, suggesting that these 10 common genes are involved in the process of pollen development while responding to HT stress. This study provides a pathway for rapidly identifying cotton pollen-specific genes that respond to HT stress. |
| first_indexed | 2024-03-09T19:41:29Z |
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| language | English |
| last_indexed | 2024-03-09T19:41:29Z |
| publishDate | 2022-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7d189a131358474db22ec4bc6d7062612023-11-24T01:38:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01236337810.3390/ijms23063378Rapid Identification of Pollen- and Anther-Specific Genes in Response to High-Temperature Stress Based on Transcriptome Profiling Analysis in CottonRui Zhang0Lili Zhou1Yanlong Li2Huanhuan Ma3Yawei Li4Yizan Ma5Rongjie Lv6Jing Yang7Weiran Wang8Aierxi Alifu9Xianlong Zhang10Jie Kong11Ling Min12Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaInstitute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaInstitute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaInstitute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaKey Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaInstitute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaKey Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, ChinaAnther indehiscence and pollen sterility caused by high temperature (HT) stress have become a major problem that decreases the yield of cotton. Pollen- and anther-specific genes play a critical role in the process of male reproduction and the response to HT stress. In order to identify pollen-specific genes that respond to HT stress, a comparative transcriptome profiling analysis was performed in the pollen and anthers of <i>Gossypium hirsutum</i> HT-sensitive Line H05 against other tissue types under normal temperature (NT) conditions, and the analysis of a differentially expressed gene was conducted in the pollen of H05 under NT and HT conditions. In total, we identified 1111 pollen-specific genes (PSGs), 1066 anther-specific genes (ASGs), and 833 pollen differentially expressed genes (DEGs). Moreover, we found that the late stage of anther included more anther- and pollen-specific genes (APSGs). Stress-related <i>cis</i>-regulatory elements (CREs) and hormone-responsive CREs are enriched in the promoters of APSGs, suggesting that APSGs may respond to HT stress. However, 833 pollen DEGs had only 10 common genes with 1111 PSGs, indicating that PSGs are mainly involved in the processes of pollen development and do not respond to HT stress. Promoters of these 10 common genes are enriched for stress-related CREs and MeJA-responsive CREs, suggesting that these 10 common genes are involved in the process of pollen development while responding to HT stress. This study provides a pathway for rapidly identifying cotton pollen-specific genes that respond to HT stress.https://www.mdpi.com/1422-0067/23/6/3378<i>Gossypium hirsutum</i>high-temperature stressmale-sterilitypollen-specific genes |
| spellingShingle | Rui Zhang Lili Zhou Yanlong Li Huanhuan Ma Yawei Li Yizan Ma Rongjie Lv Jing Yang Weiran Wang Aierxi Alifu Xianlong Zhang Jie Kong Ling Min Rapid Identification of Pollen- and Anther-Specific Genes in Response to High-Temperature Stress Based on Transcriptome Profiling Analysis in Cotton International Journal of Molecular Sciences <i>Gossypium hirsutum</i> high-temperature stress male-sterility pollen-specific genes |
| title | Rapid Identification of Pollen- and Anther-Specific Genes in Response to High-Temperature Stress Based on Transcriptome Profiling Analysis in Cotton |
| title_full | Rapid Identification of Pollen- and Anther-Specific Genes in Response to High-Temperature Stress Based on Transcriptome Profiling Analysis in Cotton |
| title_fullStr | Rapid Identification of Pollen- and Anther-Specific Genes in Response to High-Temperature Stress Based on Transcriptome Profiling Analysis in Cotton |
| title_full_unstemmed | Rapid Identification of Pollen- and Anther-Specific Genes in Response to High-Temperature Stress Based on Transcriptome Profiling Analysis in Cotton |
| title_short | Rapid Identification of Pollen- and Anther-Specific Genes in Response to High-Temperature Stress Based on Transcriptome Profiling Analysis in Cotton |
| title_sort | rapid identification of pollen and anther specific genes in response to high temperature stress based on transcriptome profiling analysis in cotton |
| topic | <i>Gossypium hirsutum</i> high-temperature stress male-sterility pollen-specific genes |
| url | https://www.mdpi.com/1422-0067/23/6/3378 |
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