Effects of Diet on the Gut Microbial Communities of Nile Tilapia (Oreochromis niloticus) Across Their Different Life Stages
Nile tilapia (Oreochromis niloticus) is one of the most important commercial freshwater fish in China, and dietary transition occurs in their different life stages. The gut microbiota is important to host health. The relationships among the diet, gut microbiota, and development of tilapia are not we...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.926132/full |
| _version_ | 1818545688268505088 |
|---|---|
| author | Miao Wang Miao Wang Zijian Fan Ziyue Zhang Mengmeng Yi Mengmeng Yi Zhigang Liu Zhigang Liu Xiaoli Ke Xiaoli Ke Fengying Gao Fengying Gao Jianmeng Cao Jianmeng Cao Maixin Lu Maixin Lu |
| author_facet | Miao Wang Miao Wang Zijian Fan Ziyue Zhang Mengmeng Yi Mengmeng Yi Zhigang Liu Zhigang Liu Xiaoli Ke Xiaoli Ke Fengying Gao Fengying Gao Jianmeng Cao Jianmeng Cao Maixin Lu Maixin Lu |
| author_sort | Miao Wang |
| collection | DOAJ |
| description | Nile tilapia (Oreochromis niloticus) is one of the most important commercial freshwater fish in China, and dietary transition occurs in their different life stages. The gut microbiota is important to host health. The relationships among the diet, gut microbiota, and development of tilapia are not well known. In the present study, we attempted to understand how diet is associated with microbiota community dynamics during the development of tilapia. The first experiment was performed under standard laboratory feeding operation to determine the effect of diet transition on intestinal microbiota. In the second experiment, tilapia were fed with Artemia or plant-based dry (PBD) food from the fish started feeding to their late juvenile stage (90 days post-fertilization). The results in the first experiment showed that feeding habit transition in juvenile fish had a low effect on the microbiota of the tilapia intestine. In the second experiment, plant-based food negatively affected the survival rate and intestinal development of tilapia. The phylum Planctomycetes was dominant in juvenile fish fed PBD food. The phylum Fusobacteria was dominant in the juvenile fish fed Artemia. At the genus level, Gemmobacter, Pirellula, and Planctomyces, belonging to the phylum Planctomycetes, were significantly abundant in the guts of fish fed the PBD food diet. Cetobacterium of the phylum Fusobacteria was dominant in juvenile fish fed Artemia. Thus, we can conclude that diet types have a great effect on the microbiota of tilapia intestine in their early life stages. The intestinal microflora of tilapia was established in juvenile tilapia, approximately 2 months after hatching. Our results provide useful information for the experimental design of studies on the microbial community of the tilapia gut. We suggest that modulation of gut microbiota of tilapia could be performed in their early life. |
| first_indexed | 2024-12-12T07:43:20Z |
| format | Article |
| id | doaj.art-7d19b9dbd906413082373ee226af1cff |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-12T07:43:20Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-7d19b9dbd906413082373ee226af1cff2022-12-22T00:32:43ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-06-01910.3389/fmars.2022.926132926132Effects of Diet on the Gut Microbial Communities of Nile Tilapia (Oreochromis niloticus) Across Their Different Life StagesMiao Wang0Miao Wang1Zijian Fan2Ziyue Zhang3Mengmeng Yi4Mengmeng Yi5Zhigang Liu6Zhigang Liu7Xiaoli Ke8Xiaoli Ke9Fengying Gao10Fengying Gao11Jianmeng Cao12Jianmeng Cao13Maixin Lu14Maixin Lu15Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou, ChinaMaoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, ChinaNile tilapia (Oreochromis niloticus) is one of the most important commercial freshwater fish in China, and dietary transition occurs in their different life stages. The gut microbiota is important to host health. The relationships among the diet, gut microbiota, and development of tilapia are not well known. In the present study, we attempted to understand how diet is associated with microbiota community dynamics during the development of tilapia. The first experiment was performed under standard laboratory feeding operation to determine the effect of diet transition on intestinal microbiota. In the second experiment, tilapia were fed with Artemia or plant-based dry (PBD) food from the fish started feeding to their late juvenile stage (90 days post-fertilization). The results in the first experiment showed that feeding habit transition in juvenile fish had a low effect on the microbiota of the tilapia intestine. In the second experiment, plant-based food negatively affected the survival rate and intestinal development of tilapia. The phylum Planctomycetes was dominant in juvenile fish fed PBD food. The phylum Fusobacteria was dominant in the juvenile fish fed Artemia. At the genus level, Gemmobacter, Pirellula, and Planctomyces, belonging to the phylum Planctomycetes, were significantly abundant in the guts of fish fed the PBD food diet. Cetobacterium of the phylum Fusobacteria was dominant in juvenile fish fed Artemia. Thus, we can conclude that diet types have a great effect on the microbiota of tilapia intestine in their early life stages. The intestinal microflora of tilapia was established in juvenile tilapia, approximately 2 months after hatching. Our results provide useful information for the experimental design of studies on the microbial community of the tilapia gut. We suggest that modulation of gut microbiota of tilapia could be performed in their early life.https://www.frontiersin.org/articles/10.3389/fmars.2022.926132/fullmicrobiotaintestinetilapiadevelopmentdiet |
| spellingShingle | Miao Wang Miao Wang Zijian Fan Ziyue Zhang Mengmeng Yi Mengmeng Yi Zhigang Liu Zhigang Liu Xiaoli Ke Xiaoli Ke Fengying Gao Fengying Gao Jianmeng Cao Jianmeng Cao Maixin Lu Maixin Lu Effects of Diet on the Gut Microbial Communities of Nile Tilapia (Oreochromis niloticus) Across Their Different Life Stages Frontiers in Marine Science microbiota intestine tilapia development diet |
| title | Effects of Diet on the Gut Microbial Communities of Nile Tilapia (Oreochromis niloticus) Across Their Different Life Stages |
| title_full | Effects of Diet on the Gut Microbial Communities of Nile Tilapia (Oreochromis niloticus) Across Their Different Life Stages |
| title_fullStr | Effects of Diet on the Gut Microbial Communities of Nile Tilapia (Oreochromis niloticus) Across Their Different Life Stages |
| title_full_unstemmed | Effects of Diet on the Gut Microbial Communities of Nile Tilapia (Oreochromis niloticus) Across Their Different Life Stages |
| title_short | Effects of Diet on the Gut Microbial Communities of Nile Tilapia (Oreochromis niloticus) Across Their Different Life Stages |
| title_sort | effects of diet on the gut microbial communities of nile tilapia oreochromis niloticus across their different life stages |
| topic | microbiota intestine tilapia development diet |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.926132/full |
| work_keys_str_mv | AT miaowang effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT miaowang effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT zijianfan effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT ziyuezhang effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT mengmengyi effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT mengmengyi effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT zhigangliu effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT zhigangliu effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT xiaolike effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT xiaolike effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT fengyinggao effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT fengyinggao effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT jianmengcao effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT jianmengcao effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT maixinlu effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages AT maixinlu effectsofdietonthegutmicrobialcommunitiesofniletilapiaoreochromisniloticusacrosstheirdifferentlifestages |