Identification of Plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation.
The nuclear proteome of Plasmodium falciparum results from the continual shuttle of proteins between the cell cytoplasm-nucleus and vice versa. Using shotgun proteomics tools, we explored the nuclear proteins of mixed populations of Plasmodium falciparum extracted from infected erythrocytes. We comb...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6209197?pdf=render |
| _version_ | 1828426518125084672 |
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| author | Sylvie Briquet Asma Ourimi Cédric Pionneau Juliana Bernardes Alessandra Carbone Solenne Chardonnet Catherine Vaquero |
| author_facet | Sylvie Briquet Asma Ourimi Cédric Pionneau Juliana Bernardes Alessandra Carbone Solenne Chardonnet Catherine Vaquero |
| author_sort | Sylvie Briquet |
| collection | DOAJ |
| description | The nuclear proteome of Plasmodium falciparum results from the continual shuttle of proteins between the cell cytoplasm-nucleus and vice versa. Using shotgun proteomics tools, we explored the nuclear proteins of mixed populations of Plasmodium falciparum extracted from infected erythrocytes. We combined GeLC-MS/MS and 2D-LC-MS/MS with a peptide ion exclusion procedure in order to increase the detection of low abundant proteins such as those involved in gene expression. We have identified 446 nuclear proteins covering all expected nuclear protein families involved in gene regulation. All structural ribosomal (40S and 60S) proteins were identified which is consistent with the nuclear localization of ribosomal biogenesis. Proteins involved in the translation machinery were also found suggesting that translational events might occur in the nucleus in P. falciparum as previously hypothesized in eukaryotes. These data were compared to the protein list established by PlasmoDB and submitted to Plasmobase a recently reported Plasmodium annotation website to propose new functional putative annotation of several unknown proteins found in the nuclear extracts. |
| first_indexed | 2024-12-10T16:47:19Z |
| format | Article |
| id | doaj.art-7d1ec337a8b54c7483b8693cfaecb794 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T16:47:19Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7d1ec337a8b54c7483b8693cfaecb7942022-12-22T01:41:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011310e020559610.1371/journal.pone.0205596Identification of Plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation.Sylvie BriquetAsma OurimiCédric PionneauJuliana BernardesAlessandra CarboneSolenne ChardonnetCatherine VaqueroThe nuclear proteome of Plasmodium falciparum results from the continual shuttle of proteins between the cell cytoplasm-nucleus and vice versa. Using shotgun proteomics tools, we explored the nuclear proteins of mixed populations of Plasmodium falciparum extracted from infected erythrocytes. We combined GeLC-MS/MS and 2D-LC-MS/MS with a peptide ion exclusion procedure in order to increase the detection of low abundant proteins such as those involved in gene expression. We have identified 446 nuclear proteins covering all expected nuclear protein families involved in gene regulation. All structural ribosomal (40S and 60S) proteins were identified which is consistent with the nuclear localization of ribosomal biogenesis. Proteins involved in the translation machinery were also found suggesting that translational events might occur in the nucleus in P. falciparum as previously hypothesized in eukaryotes. These data were compared to the protein list established by PlasmoDB and submitted to Plasmobase a recently reported Plasmodium annotation website to propose new functional putative annotation of several unknown proteins found in the nuclear extracts.http://europepmc.org/articles/PMC6209197?pdf=render |
| spellingShingle | Sylvie Briquet Asma Ourimi Cédric Pionneau Juliana Bernardes Alessandra Carbone Solenne Chardonnet Catherine Vaquero Identification of Plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation. PLoS ONE |
| title | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation. |
| title_full | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation. |
| title_fullStr | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation. |
| title_full_unstemmed | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation. |
| title_short | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation. |
| title_sort | identification of plasmodium falciparum nuclear proteins by mass spectrometry and proposed protein annotation |
| url | http://europepmc.org/articles/PMC6209197?pdf=render |
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