1,2,3,4,6-Penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced Obesity
Phytochemicals that interrupt adipocyte lifecycle can provide anti-obesity effects. 1,2,3,4,6-penta-O-galloyl-<span style="font-variant: small-caps;">d</span>-glucose (PGG) is a tannin with two isomers that occurs widely in plants and exhibits various pharmacological activities...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/7/4052 |
| _version_ | 1797438870732668928 |
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| author | Ashish Rao Sathyanarayana Chung-Kuang Lu Chih-Chuang Liaw Chia-Chuan Chang Hsin-Ying Han Brian D. Green Wei-Jan Huang Cheng Huang Wen-Di He Lin-Chien Lee Hui-Kang Liu |
| author_facet | Ashish Rao Sathyanarayana Chung-Kuang Lu Chih-Chuang Liaw Chia-Chuan Chang Hsin-Ying Han Brian D. Green Wei-Jan Huang Cheng Huang Wen-Di He Lin-Chien Lee Hui-Kang Liu |
| author_sort | Ashish Rao Sathyanarayana |
| collection | DOAJ |
| description | Phytochemicals that interrupt adipocyte lifecycle can provide anti-obesity effects. 1,2,3,4,6-penta-O-galloyl-<span style="font-variant: small-caps;">d</span>-glucose (PGG) is a tannin with two isomers that occurs widely in plants and exhibits various pharmacological activities. The aim of the investigation is to comprehensively examine effects of PGG isomer(s) on adipocyte lifecycle and diet-induced obesity. Human mesenchymal stem cells (hMSC), 3T3-L1 fibroblasts, and H4IIE hepatoma cells were used to determine the effects of PGG isomers on cell viability and adipogenesis. Mice with diet-induced obesity were generated from male C57/BL6 mice fed with a 45% high fat diet. Oral administration of β-PGG (0.1 and 5 mg/kg) lasted for 14 weeks. Viability was reduced by repeated PGG treatment in hMSC, preadipocytes, and cells under differentiation. PGG mainly induces apoptosis, and this effect is independent of its insulin mimetic action. In vivo, administration of β-PGG attenuated shortening of the colon, hyperlipidaemia, fat cells and islet hypertrophy in DIO mice. Hepatic steatosis and related gene expression were improved along with glucose intolerance. Increased serum adiponectin, leptin, and glucagon-like peptide-1 levels were also observed. In conclusion, repeated PGG treatment interrupts the adipocyte lifecycle. PGG administration reduces adiposity and fatty liver development in DIO mice, and therefore, PGG could aid in clinical management of obesity. |
| first_indexed | 2024-03-09T11:44:33Z |
| format | Article |
| id | doaj.art-7d1fa2115ead4cf4958b468e156dafe8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T11:44:33Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7d1fa2115ead4cf4958b468e156dafe82023-11-30T23:26:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01237405210.3390/ijms230740521,2,3,4,6-Penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced ObesityAshish Rao Sathyanarayana0Chung-Kuang Lu1Chih-Chuang Liaw2Chia-Chuan Chang3Hsin-Ying Han4Brian D. Green5Wei-Jan Huang6Cheng Huang7Wen-Di He8Lin-Chien Lee9Hui-Kang Liu10Ph.D. Program for the Clinical Drug Discovery from Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei 110, TaiwanDivision of Chinese Medicinal Chemistry, National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei 112, TaiwanDepartment of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, TaiwanDepartment of Pharmacy, National Taiwan University, Taipei 10617, TaiwanDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 202, TaiwanInstitute for Global Food Security, School of Biological Sciences, Queen’s University Belfast, Belfast BT7 1NN, UKPh.D. Program for the Clinical Drug Discovery from Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei 110, TaiwanDepartment of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei 112, TaiwanDepartment of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, TaiwanDepartment of Physical Medicine and Rehabilitation, Cheng Hsin General Hospital, 45, Cheng Hsin Street, Taipei 112, TaiwanPh.D. Program for the Clinical Drug Discovery from Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei 110, TaiwanPhytochemicals that interrupt adipocyte lifecycle can provide anti-obesity effects. 1,2,3,4,6-penta-O-galloyl-<span style="font-variant: small-caps;">d</span>-glucose (PGG) is a tannin with two isomers that occurs widely in plants and exhibits various pharmacological activities. The aim of the investigation is to comprehensively examine effects of PGG isomer(s) on adipocyte lifecycle and diet-induced obesity. Human mesenchymal stem cells (hMSC), 3T3-L1 fibroblasts, and H4IIE hepatoma cells were used to determine the effects of PGG isomers on cell viability and adipogenesis. Mice with diet-induced obesity were generated from male C57/BL6 mice fed with a 45% high fat diet. Oral administration of β-PGG (0.1 and 5 mg/kg) lasted for 14 weeks. Viability was reduced by repeated PGG treatment in hMSC, preadipocytes, and cells under differentiation. PGG mainly induces apoptosis, and this effect is independent of its insulin mimetic action. In vivo, administration of β-PGG attenuated shortening of the colon, hyperlipidaemia, fat cells and islet hypertrophy in DIO mice. Hepatic steatosis and related gene expression were improved along with glucose intolerance. Increased serum adiponectin, leptin, and glucagon-like peptide-1 levels were also observed. In conclusion, repeated PGG treatment interrupts the adipocyte lifecycle. PGG administration reduces adiposity and fatty liver development in DIO mice, and therefore, PGG could aid in clinical management of obesity.https://www.mdpi.com/1422-0067/23/7/40521,2,3,4,6-penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucoseobesityadipocyte lifecycleapoptosishepatic steatohepatitis |
| spellingShingle | Ashish Rao Sathyanarayana Chung-Kuang Lu Chih-Chuang Liaw Chia-Chuan Chang Hsin-Ying Han Brian D. Green Wei-Jan Huang Cheng Huang Wen-Di He Lin-Chien Lee Hui-Kang Liu 1,2,3,4,6-Penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced Obesity International Journal of Molecular Sciences 1,2,3,4,6-penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucose obesity adipocyte lifecycle apoptosis hepatic steatohepatitis |
| title | 1,2,3,4,6-Penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced Obesity |
| title_full | 1,2,3,4,6-Penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced Obesity |
| title_fullStr | 1,2,3,4,6-Penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced Obesity |
| title_full_unstemmed | 1,2,3,4,6-Penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced Obesity |
| title_short | 1,2,3,4,6-Penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucose Interrupts the Early Adipocyte Lifecycle and Attenuates Adiposity and Hepatic Steatosis in Mice with Diet-Induced Obesity |
| title_sort | 1 2 3 4 6 penta o galloyl span style font variant small caps d span glucose interrupts the early adipocyte lifecycle and attenuates adiposity and hepatic steatosis in mice with diet induced obesity |
| topic | 1,2,3,4,6-penta-O-galloyl-<span style="font-variant: small-caps">d</span>-glucose obesity adipocyte lifecycle apoptosis hepatic steatohepatitis |
| url | https://www.mdpi.com/1422-0067/23/7/4052 |
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