Comparative evaluation of in vitro techniques for predicting metabolizable energy content of total mixed ration for Murrah buffaloes
The present study was conducted to ascertain the sensitivity of in vitro techniques namely, in vitro gas production (IVGPT) and Cornell Net Carbohydrate and Protein System (CNCPS) fraction for predicting the metabolizable energy (ME) content of total mixed ration for Murrah buffaloes. The total mix...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2021-08-01
|
| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/114147 |
| _version_ | 1797897025194295296 |
|---|---|
| author | NEETI LAKHANI NITIN TYAGI |
| author_facet | NEETI LAKHANI NITIN TYAGI |
| author_sort | NEETI LAKHANI |
| collection | DOAJ |
| description |
The present study was conducted to ascertain the sensitivity of in vitro techniques namely, in vitro gas production (IVGPT) and Cornell Net Carbohydrate and Protein System (CNCPS) fraction for predicting the metabolizable energy (ME) content of total mixed ration for Murrah buffaloes. The total mixed ration forming dietary treatment were TMR1, TMR2 and TMR3 having mixture of maize silage and concentrate in ratio of 30:70, 40:60 and 50:50, respectively. The diets formulated were isocaloric and isonitrogenous. The in vitro gas production was studied by incubation of diet with rumen liquor as inoculum to predict the metabolizable energy content of ruminant feeds. The CNCPS fraction analysing ME content of feed samples was carried out in laboratory. Lactating Murrah buffaloes (n = 18) having similar lactation yield (MY 9 kg±2.5) were allotted to three groups in a completely randomised design and fed TMR as per the dietary treatments. The feeding trial was carried out for 3 months and nutrient utilization was analysed. The efficiency of ME utilization in vivo were compared to in vitro gas production and CNCPS fraction. Correlation coefficient (r) between in vitro techniques and in vivo trial in evaluating ME content demonstrated a significantly (P<0.01) high correlation of 0.734 with IVGPT and 0.752 with CNCPS fraction indicating that both in vitro techniques were reliable in predicting metabolizable energy content of feedstuffs for ruminant feeding.
|
| first_indexed | 2024-04-10T07:51:02Z |
| format | Article |
| id | doaj.art-7d22b282810242c392c885094835cb55 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:51:02Z |
| publishDate | 2021-08-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-7d22b282810242c392c885094835cb552023-02-23T10:12:33ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272021-08-0191310.56093/ijans.v91i3.114147Comparative evaluation of in vitro techniques for predicting metabolizable energy content of total mixed ration for Murrah buffaloesNEETI LAKHANI0NITIN TYAGI1ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India The present study was conducted to ascertain the sensitivity of in vitro techniques namely, in vitro gas production (IVGPT) and Cornell Net Carbohydrate and Protein System (CNCPS) fraction for predicting the metabolizable energy (ME) content of total mixed ration for Murrah buffaloes. The total mixed ration forming dietary treatment were TMR1, TMR2 and TMR3 having mixture of maize silage and concentrate in ratio of 30:70, 40:60 and 50:50, respectively. The diets formulated were isocaloric and isonitrogenous. The in vitro gas production was studied by incubation of diet with rumen liquor as inoculum to predict the metabolizable energy content of ruminant feeds. The CNCPS fraction analysing ME content of feed samples was carried out in laboratory. Lactating Murrah buffaloes (n = 18) having similar lactation yield (MY 9 kg±2.5) were allotted to three groups in a completely randomised design and fed TMR as per the dietary treatments. The feeding trial was carried out for 3 months and nutrient utilization was analysed. The efficiency of ME utilization in vivo were compared to in vitro gas production and CNCPS fraction. Correlation coefficient (r) between in vitro techniques and in vivo trial in evaluating ME content demonstrated a significantly (P<0.01) high correlation of 0.734 with IVGPT and 0.752 with CNCPS fraction indicating that both in vitro techniques were reliable in predicting metabolizable energy content of feedstuffs for ruminant feeding. https://epubs.icar.org.in/index.php/IJAnS/article/view/114147CNCPSIn vitro gas productionMetabolizable energyTotal mixed ration |
| spellingShingle | NEETI LAKHANI NITIN TYAGI Comparative evaluation of in vitro techniques for predicting metabolizable energy content of total mixed ration for Murrah buffaloes Indian Journal of Animal Sciences CNCPS In vitro gas production Metabolizable energy Total mixed ration |
| title | Comparative evaluation of in vitro techniques for predicting metabolizable energy content of total mixed ration for Murrah buffaloes |
| title_full | Comparative evaluation of in vitro techniques for predicting metabolizable energy content of total mixed ration for Murrah buffaloes |
| title_fullStr | Comparative evaluation of in vitro techniques for predicting metabolizable energy content of total mixed ration for Murrah buffaloes |
| title_full_unstemmed | Comparative evaluation of in vitro techniques for predicting metabolizable energy content of total mixed ration for Murrah buffaloes |
| title_short | Comparative evaluation of in vitro techniques for predicting metabolizable energy content of total mixed ration for Murrah buffaloes |
| title_sort | comparative evaluation of in vitro techniques for predicting metabolizable energy content of total mixed ration for murrah buffaloes |
| topic | CNCPS In vitro gas production Metabolizable energy Total mixed ration |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/114147 |
| work_keys_str_mv | AT neetilakhani comparativeevaluationofinvitrotechniquesforpredictingmetabolizableenergycontentoftotalmixedrationformurrahbuffaloes AT nitintyagi comparativeevaluationofinvitrotechniquesforpredictingmetabolizableenergycontentoftotalmixedrationformurrahbuffaloes |