Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance
Compatibility of each strain in a multi-strain probiotic (MSP), along with its properties, becomes a strong base for its formulation. In this study, single-strain probiotics (SSPs) and multi-strain probiotics (MSPs) were evaluated in vitro for strain compatibility, microbial antagonism, biofilm form...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/11/1644 |
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| author | Puvaneswari Puvanasundram Chou Min Chong Suriana Sabri Md Sabri Mohd Yusoff Keng Chin Lim Murni Karim |
| author_facet | Puvaneswari Puvanasundram Chou Min Chong Suriana Sabri Md Sabri Mohd Yusoff Keng Chin Lim Murni Karim |
| author_sort | Puvaneswari Puvanasundram |
| collection | DOAJ |
| description | Compatibility of each strain in a multi-strain probiotic (MSP), along with its properties, becomes a strong base for its formulation. In this study, single-strain probiotics (SSPs) and multi-strain probiotics (MSPs) were evaluated in vitro for strain compatibility, microbial antagonism, biofilm formation capacity, and stress tolerance. <i>Bacillus amyloliquefaciens</i> L11, <i>Enterococcus hirae</i> LAB3, and <i>Lysinibacillus fusiformis</i> SPS11 were chosen as MSP1 candidates because they showed much stronger antagonism to <i>Aeromonas hydrophila</i> and <i>Streptococcus agalactiae</i> than a single probiotic. MSP 2 candidates were <i>Lysinibacillus fusiformis</i> strains SPS11, A1, and <i>Lysinibacillus sphaericus</i> strain NAS32 because the inhibition zone produced by MSP 2 against <i>Vibrio harveyi</i> and <i>Vibrio parahaemolyticus</i> was much higher than that produced by its constituent SSPs. MSP1 in the co-culture assay reduced (<i>p</i> < 0.05) <i>A. hydrophila</i> count from 9.89 ± 0.1 CFU mL<sup>−1</sup> to 2.14 ± 0.2 CFU mL<sup>−1</sup>. The biofilm formation of both MSPs were significantly higher (<i>p</i> < 0.05) than its constituent SSPs and the pathogens. The SSPs in both MSPs generally showed resistance to high temperatures (80, 90, and 100 °C) and a wide range of pH (2 to 9). This in vitro assessment study demonstrates that MSP1 and 2 have the potential to be further explored as multi-strain probiotics on selected aquatic species. |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T19:15:15Z |
| publishDate | 2022-11-01 |
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| spelling | doaj.art-7d26e2e70acd45d48a1f2369f73d6ce42023-11-24T03:48:47ZengMDPI AGBiology2079-77372022-11-011111164410.3390/biology11111644Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress TolerancePuvaneswari Puvanasundram0Chou Min Chong1Suriana Sabri2Md Sabri Mohd Yusoff3Keng Chin Lim4Murni Karim5Laboratory of Aquatic Animal Health and Therapeutics, Institute of Biosciences, University Putra Malaysia, Serdang 43400, Selangor, MalaysiaLaboratory of Aquatic Animal Health and Therapeutics, Institute of Biosciences, University Putra Malaysia, Serdang 43400, Selangor, MalaysiaEnzyme and Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Serdang 43400, Selangor, MalaysiaDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaDepartment of Aquaculture, Faculty of Agriculture, University Putra Malaysia, Serdang 43400, Selangor, MalaysiaDepartment of Aquaculture, Faculty of Agriculture, University Putra Malaysia, Serdang 43400, Selangor, MalaysiaCompatibility of each strain in a multi-strain probiotic (MSP), along with its properties, becomes a strong base for its formulation. In this study, single-strain probiotics (SSPs) and multi-strain probiotics (MSPs) were evaluated in vitro for strain compatibility, microbial antagonism, biofilm formation capacity, and stress tolerance. <i>Bacillus amyloliquefaciens</i> L11, <i>Enterococcus hirae</i> LAB3, and <i>Lysinibacillus fusiformis</i> SPS11 were chosen as MSP1 candidates because they showed much stronger antagonism to <i>Aeromonas hydrophila</i> and <i>Streptococcus agalactiae</i> than a single probiotic. MSP 2 candidates were <i>Lysinibacillus fusiformis</i> strains SPS11, A1, and <i>Lysinibacillus sphaericus</i> strain NAS32 because the inhibition zone produced by MSP 2 against <i>Vibrio harveyi</i> and <i>Vibrio parahaemolyticus</i> was much higher than that produced by its constituent SSPs. MSP1 in the co-culture assay reduced (<i>p</i> < 0.05) <i>A. hydrophila</i> count from 9.89 ± 0.1 CFU mL<sup>−1</sup> to 2.14 ± 0.2 CFU mL<sup>−1</sup>. The biofilm formation of both MSPs were significantly higher (<i>p</i> < 0.05) than its constituent SSPs and the pathogens. The SSPs in both MSPs generally showed resistance to high temperatures (80, 90, and 100 °C) and a wide range of pH (2 to 9). This in vitro assessment study demonstrates that MSP1 and 2 have the potential to be further explored as multi-strain probiotics on selected aquatic species.https://www.mdpi.com/2079-7737/11/11/1644multi-strain probiotics (MSP)single-strain probiotics (SSPs)antagonismstrain compatibilitypropertiesbiofilm |
| spellingShingle | Puvaneswari Puvanasundram Chou Min Chong Suriana Sabri Md Sabri Mohd Yusoff Keng Chin Lim Murni Karim Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance Biology multi-strain probiotics (MSP) single-strain probiotics (SSPs) antagonism strain compatibility properties biofilm |
| title | Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance |
| title_full | Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance |
| title_fullStr | Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance |
| title_full_unstemmed | Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance |
| title_short | Efficacy of Single and Multi-Strain Probiotics on In Vitro Strain Compatibility, Pathogen Inhibition, Biofilm Formation Capability, and Stress Tolerance |
| title_sort | efficacy of single and multi strain probiotics on in vitro strain compatibility pathogen inhibition biofilm formation capability and stress tolerance |
| topic | multi-strain probiotics (MSP) single-strain probiotics (SSPs) antagonism strain compatibility properties biofilm |
| url | https://www.mdpi.com/2079-7737/11/11/1644 |
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