Pirfenidone Protects from UVB-Induced Photodamage in Hairless Mice
Background: Ultraviolet radiation (UV) is the main environmental factor that causes histological degenerative changes of the skin giving rise to a chronic process called photodamage. Non-melanoma skin cancer induced by UVB radiation is a result of a cascade of molecular events caused by DNA damage i...
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MDPI AG
2023-03-01
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author | Yocasta Martinez-Alvarado Eduardo Amezcua-Galvez Judith Davila-Rodriguez Ana Sandoval-Rodriguez Marina Galicia-Moreno Mónica Almeida-López Silvia Lucano-Landeros Arturo Santos Hugo Christian Monroy-Ramirez Juan Armendariz-Borunda |
author_facet | Yocasta Martinez-Alvarado Eduardo Amezcua-Galvez Judith Davila-Rodriguez Ana Sandoval-Rodriguez Marina Galicia-Moreno Mónica Almeida-López Silvia Lucano-Landeros Arturo Santos Hugo Christian Monroy-Ramirez Juan Armendariz-Borunda |
author_sort | Yocasta Martinez-Alvarado |
collection | DOAJ |
description | Background: Ultraviolet radiation (UV) is the main environmental factor that causes histological degenerative changes of the skin giving rise to a chronic process called photodamage. Non-melanoma skin cancer induced by UVB radiation is a result of a cascade of molecular events caused by DNA damage in epidermis cells, including persistent inflammation, oxidative stress, and suppression of T cell-mediated immunity. Retinoids such as tretinoin have been widely used in skin to treat photoaging and photodamage, though its secondary adverse effects have been recognized. Pirfenidone (PFD) has emerged as an antifibrogenic, anti-inflammatory and antioxidant agent, and in this work its efficacy was evaluated in a model of UVB-induced photodamage. Methods: Epidermal, dermal, and inflammatory changes were measured by histomorphometric parameters. In addition, gene, and protein expression of key molecules in these processes were evaluated. Results: Our results revealed an anti-photodamage effect of topical PFD with absence of inflammatory skin lesions determined by dermoscopy. In addition, PFD reduced elastosis, improved organization, arrangement, and deposition of dermal collagens, downregulated several pro-inflammatory markers such as NF-kB, IL-1, IL-6 and TNFα, and decreased keratinocyte damage. Conclusion: Topical pirfenidone represents a promising agent for the treatment of cell photodamage in humans. Clinical trials need to be carried out to explore this premise. |
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language | English |
last_indexed | 2024-03-11T05:30:47Z |
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series | Molecules |
spelling | doaj.art-7d2863a0e14d42d78c9daeade4b6ebb72023-11-17T17:11:01ZengMDPI AGMolecules1420-30492023-03-01287292910.3390/molecules28072929Pirfenidone Protects from UVB-Induced Photodamage in Hairless MiceYocasta Martinez-Alvarado0Eduardo Amezcua-Galvez1Judith Davila-Rodriguez2Ana Sandoval-Rodriguez3Marina Galicia-Moreno4Mónica Almeida-López5Silvia Lucano-Landeros6Arturo Santos7Hugo Christian Monroy-Ramirez8Juan Armendariz-Borunda9Programa de Doctorado en Ciencias en Biología Molecular en Medicina, CUCS, University of Guadalajara, Guadalajara 44340, MexicoDepartment of Pathology, Civil Hospital of Guadalajara “Fray Antonio Alcalde”, Guadalajara 44280, MexicoDepartment of Pathology, Civil Hospital of Guadalajara “Fray Antonio Alcalde”, Guadalajara 44280, MexicoDepartment of Molecular Biology and Genomics, Institute of Molecular Biology in Medicine and Gene Therapy, CUCS, University of Guadalajara, Guadalajara 44340, MexicoDepartment of Molecular Biology and Genomics, Institute of Molecular Biology in Medicine and Gene Therapy, CUCS, University of Guadalajara, Guadalajara 44340, MexicoDepartment of Basic Psychology, CUCS, University of Guadalajara, Guadalajara 44340, MexicoDepartment of Molecular Biology and Genomics, Institute of Molecular Biology in Medicine and Gene Therapy, CUCS, University of Guadalajara, Guadalajara 44340, MexicoSchool of Medicine and Health Sciences, Tecnologico de Monterrey, Zapopan 45138, MexicoDepartment of Molecular Biology and Genomics, Institute of Molecular Biology in Medicine and Gene Therapy, CUCS, University of Guadalajara, Guadalajara 44340, MexicoDepartment of Molecular Biology and Genomics, Institute of Molecular Biology in Medicine and Gene Therapy, CUCS, University of Guadalajara, Guadalajara 44340, MexicoBackground: Ultraviolet radiation (UV) is the main environmental factor that causes histological degenerative changes of the skin giving rise to a chronic process called photodamage. Non-melanoma skin cancer induced by UVB radiation is a result of a cascade of molecular events caused by DNA damage in epidermis cells, including persistent inflammation, oxidative stress, and suppression of T cell-mediated immunity. Retinoids such as tretinoin have been widely used in skin to treat photoaging and photodamage, though its secondary adverse effects have been recognized. Pirfenidone (PFD) has emerged as an antifibrogenic, anti-inflammatory and antioxidant agent, and in this work its efficacy was evaluated in a model of UVB-induced photodamage. Methods: Epidermal, dermal, and inflammatory changes were measured by histomorphometric parameters. In addition, gene, and protein expression of key molecules in these processes were evaluated. Results: Our results revealed an anti-photodamage effect of topical PFD with absence of inflammatory skin lesions determined by dermoscopy. In addition, PFD reduced elastosis, improved organization, arrangement, and deposition of dermal collagens, downregulated several pro-inflammatory markers such as NF-kB, IL-1, IL-6 and TNFα, and decreased keratinocyte damage. Conclusion: Topical pirfenidone represents a promising agent for the treatment of cell photodamage in humans. Clinical trials need to be carried out to explore this premise.https://www.mdpi.com/1420-3049/28/7/2929photodamagepro-inflammatory markersultraviolet radiationskinpirfenidone |
spellingShingle | Yocasta Martinez-Alvarado Eduardo Amezcua-Galvez Judith Davila-Rodriguez Ana Sandoval-Rodriguez Marina Galicia-Moreno Mónica Almeida-López Silvia Lucano-Landeros Arturo Santos Hugo Christian Monroy-Ramirez Juan Armendariz-Borunda Pirfenidone Protects from UVB-Induced Photodamage in Hairless Mice Molecules photodamage pro-inflammatory markers ultraviolet radiation skin pirfenidone |
title | Pirfenidone Protects from UVB-Induced Photodamage in Hairless Mice |
title_full | Pirfenidone Protects from UVB-Induced Photodamage in Hairless Mice |
title_fullStr | Pirfenidone Protects from UVB-Induced Photodamage in Hairless Mice |
title_full_unstemmed | Pirfenidone Protects from UVB-Induced Photodamage in Hairless Mice |
title_short | Pirfenidone Protects from UVB-Induced Photodamage in Hairless Mice |
title_sort | pirfenidone protects from uvb induced photodamage in hairless mice |
topic | photodamage pro-inflammatory markers ultraviolet radiation skin pirfenidone |
url | https://www.mdpi.com/1420-3049/28/7/2929 |
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