Characterization Of Tetracycline Resistance Of Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia
Resistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET,...
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| Format: | Article |
| Language: | English |
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Sciendo
2015-12-01
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| Series: | Acta Veterinaria |
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| Online Access: | https://doi.org/10.1515/acve-2015-0046 |
| _version_ | 1818651901135159296 |
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| author | Todorović Dalibor Velhner Maja Milanov Dubravka Vidanović Dejan Suvajdžić Ljiljana Stojanov Igor Krnjaić Dejan |
| author_facet | Todorović Dalibor Velhner Maja Milanov Dubravka Vidanović Dejan Suvajdžić Ljiljana Stojanov Igor Krnjaić Dejan |
| author_sort | Todorović Dalibor |
| collection | DOAJ |
| description | Resistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET, ranged from 1-256 mg/L. Namely, 13 isolates exhibited MIC to TET at 256 mg/L, in four of the isolates, the MIC was 128 mg/L and one isolate had MIC 64 mg/L. Ten isolates were exhibiting a MIC of 1mg/L. It was evident that Salmonella Infantis had also spread to breeders and layers. In this work, we detected the tetA gene and the corresponding tetR gene (encoding the repressor protein) as well as the truncated transposon Tn1721, which are responsible for the resistance to TET. The presence of the non conjugative transposons from the conjugative plasmid has facilitated the spread of resistance to TET in Salmonella. It was concluded that higher biosecurity practice in poultry farming presents the best option to eliminate infections caused by Salmonella spp. from poultry flocks in Serbia. A rational use of antimicrobials is necessary to prevent any further spread of Salmonella Infantis resistant clones. |
| first_indexed | 2024-12-17T02:13:28Z |
| format | Article |
| id | doaj.art-7d28c7fdc01c48578b28f6d79c1414a6 |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-12-17T02:13:28Z |
| publishDate | 2015-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj.art-7d28c7fdc01c48578b28f6d79c1414a62022-12-21T22:07:29ZengSciendoActa Veterinaria1820-74482015-12-0165454855610.1515/acve-2015-0046Characterization Of Tetracycline Resistance Of Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated From Poultry In The Northern Part Of SerbiaTodorović Dalibor0Velhner Maja1Milanov Dubravka2Vidanović Dejan3Suvajdžić Ljiljana4Stojanov Igor5Krnjaić Dejan6Scientific Veterinary Institute „Novi Sad“, Novi Sad, Republic of SerbiaScientific Veterinary Institute „Novi Sad“, Novi Sad, Republic of SerbiaScientific Veterinary Institute „Novi Sad“, Novi Sad, Republic of SerbiaVeterinary Institute Kraljevo, Kraljevo, Republic of SerbiaFaculty of Medicine, Department of Pharmacy, University of Novi Sad, Republic of SerbiaScientific Veterinary Institute „Novi Sad“, Novi Sad, Republic of SerbiaFaculty of Veterinary Medicine Belgrade, University of Belgrade, Republic of SerbiaResistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET, ranged from 1-256 mg/L. Namely, 13 isolates exhibited MIC to TET at 256 mg/L, in four of the isolates, the MIC was 128 mg/L and one isolate had MIC 64 mg/L. Ten isolates were exhibiting a MIC of 1mg/L. It was evident that Salmonella Infantis had also spread to breeders and layers. In this work, we detected the tetA gene and the corresponding tetR gene (encoding the repressor protein) as well as the truncated transposon Tn1721, which are responsible for the resistance to TET. The presence of the non conjugative transposons from the conjugative plasmid has facilitated the spread of resistance to TET in Salmonella. It was concluded that higher biosecurity practice in poultry farming presents the best option to eliminate infections caused by Salmonella spp. from poultry flocks in Serbia. A rational use of antimicrobials is necessary to prevent any further spread of Salmonella Infantis resistant clones.https://doi.org/10.1515/acve-2015-0046poultrysalmonella infantistetracyclineteta genetetr genetransposone tn1721 |
| spellingShingle | Todorović Dalibor Velhner Maja Milanov Dubravka Vidanović Dejan Suvajdžić Ljiljana Stojanov Igor Krnjaić Dejan Characterization Of Tetracycline Resistance Of Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia Acta Veterinaria poultry salmonella infantis tetracycline teta gene tetr gene transposone tn1721 |
| title | Characterization Of Tetracycline Resistance Of Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia |
| title_full | Characterization Of Tetracycline Resistance Of Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia |
| title_fullStr | Characterization Of Tetracycline Resistance Of Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia |
| title_full_unstemmed | Characterization Of Tetracycline Resistance Of Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia |
| title_short | Characterization Of Tetracycline Resistance Of Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia |
| title_sort | characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of serbia |
| topic | poultry salmonella infantis tetracycline teta gene tetr gene transposone tn1721 |
| url | https://doi.org/10.1515/acve-2015-0046 |
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