Metabolomic Response of the Creeping Wood Sorrel <i>Oxalis corniculata</i> to Low-Dose Radiation Exposure from Fukushima’s Contaminated Soil
The biological consequences of the Fukushima nuclear accident have been intensively studied using the pale grass blue butterfly <i>Zizeeria maha</i> and its host plant, the creeping wood sorrel <i>Oxalis corniculata</i>. Here, we performed metabolomic analyses of <i>Oxa...
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MDPI AG
2021-09-01
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| author | Ko Sakauchi Wataru Taira Joji M. Otaki |
| author_facet | Ko Sakauchi Wataru Taira Joji M. Otaki |
| author_sort | Ko Sakauchi |
| collection | DOAJ |
| description | The biological consequences of the Fukushima nuclear accident have been intensively studied using the pale grass blue butterfly <i>Zizeeria maha</i> and its host plant, the creeping wood sorrel <i>Oxalis corniculata</i>. Here, we performed metabolomic analyses of <i>Oxalis</i> leaves from Okinawa to examine the plant metabolites that were upregulated or downregulated in response to low-dose radiation exposure from Fukushima’s contaminated soil. The cumulative dose of radiation to the plants was 5.7 mGy (34 μGy/h for 7 days). The GC-MS analysis revealed a systematic tendency of downregulation among the metabolites, some of which were annotated as caproic acid, nonanoic acid, azelaic acid, and oleic acid. Others were annotated as fructose, glucose, and citric acid, involved in the carbohydrate metabolic pathways. Notably, the peak annotated as lauric acid was upregulated. In contrast, the LC-MS analysis detected many upregulated metabolites, some of which were annotated as either antioxidants or stress-related chemicals involved in defense pathways. Among them, only three metabolite peaks had a single annotation, one of which was alfuzosin, an antagonist of the α<sub>1</sub>-adrenergic receptor. We conclude that this <i>Oxalis</i> plant responded metabolically to low-dose radiation exposure from Fukushima’s contaminated soil, which may mediate the ecological “field effects” of the developmental deterioration of butterflies in Fukushima. |
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| spelling | doaj.art-7d2a2bb1ded44541849b8187a59ee4092023-11-22T13:56:58ZengMDPI AGLife2075-17292021-09-0111999010.3390/life11090990Metabolomic Response of the Creeping Wood Sorrel <i>Oxalis corniculata</i> to Low-Dose Radiation Exposure from Fukushima’s Contaminated SoilKo Sakauchi0Wataru Taira1Joji M. Otaki2The BCPH Unit of Molecular Physiology, Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Okinawa 903-0213, JapanThe BCPH Unit of Molecular Physiology, Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Okinawa 903-0213, JapanThe BCPH Unit of Molecular Physiology, Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Okinawa 903-0213, JapanThe biological consequences of the Fukushima nuclear accident have been intensively studied using the pale grass blue butterfly <i>Zizeeria maha</i> and its host plant, the creeping wood sorrel <i>Oxalis corniculata</i>. Here, we performed metabolomic analyses of <i>Oxalis</i> leaves from Okinawa to examine the plant metabolites that were upregulated or downregulated in response to low-dose radiation exposure from Fukushima’s contaminated soil. The cumulative dose of radiation to the plants was 5.7 mGy (34 μGy/h for 7 days). The GC-MS analysis revealed a systematic tendency of downregulation among the metabolites, some of which were annotated as caproic acid, nonanoic acid, azelaic acid, and oleic acid. Others were annotated as fructose, glucose, and citric acid, involved in the carbohydrate metabolic pathways. Notably, the peak annotated as lauric acid was upregulated. In contrast, the LC-MS analysis detected many upregulated metabolites, some of which were annotated as either antioxidants or stress-related chemicals involved in defense pathways. Among them, only three metabolite peaks had a single annotation, one of which was alfuzosin, an antagonist of the α<sub>1</sub>-adrenergic receptor. We conclude that this <i>Oxalis</i> plant responded metabolically to low-dose radiation exposure from Fukushima’s contaminated soil, which may mediate the ecological “field effects” of the developmental deterioration of butterflies in Fukushima.https://www.mdpi.com/2075-1729/11/9/990metabolomeGC-MSLC-MSFukushima nuclear accidentlow-dose radiation exposureplant physiology |
| spellingShingle | Ko Sakauchi Wataru Taira Joji M. Otaki Metabolomic Response of the Creeping Wood Sorrel <i>Oxalis corniculata</i> to Low-Dose Radiation Exposure from Fukushima’s Contaminated Soil Life metabolome GC-MS LC-MS Fukushima nuclear accident low-dose radiation exposure plant physiology |
| title | Metabolomic Response of the Creeping Wood Sorrel <i>Oxalis corniculata</i> to Low-Dose Radiation Exposure from Fukushima’s Contaminated Soil |
| title_full | Metabolomic Response of the Creeping Wood Sorrel <i>Oxalis corniculata</i> to Low-Dose Radiation Exposure from Fukushima’s Contaminated Soil |
| title_fullStr | Metabolomic Response of the Creeping Wood Sorrel <i>Oxalis corniculata</i> to Low-Dose Radiation Exposure from Fukushima’s Contaminated Soil |
| title_full_unstemmed | Metabolomic Response of the Creeping Wood Sorrel <i>Oxalis corniculata</i> to Low-Dose Radiation Exposure from Fukushima’s Contaminated Soil |
| title_short | Metabolomic Response of the Creeping Wood Sorrel <i>Oxalis corniculata</i> to Low-Dose Radiation Exposure from Fukushima’s Contaminated Soil |
| title_sort | metabolomic response of the creeping wood sorrel i oxalis corniculata i to low dose radiation exposure from fukushima s contaminated soil |
| topic | metabolome GC-MS LC-MS Fukushima nuclear accident low-dose radiation exposure plant physiology |
| url | https://www.mdpi.com/2075-1729/11/9/990 |
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