Pentagone internalises glypicans to fine-tune multiple signalling pathways
Tight regulation of signalling activity is crucial for proper tissue patterning and growth. Here we investigate the function of Pentagone (Pent), a secreted protein that acts in a regulatory feedback during establishment and maintenance of BMP/Dpp morphogen signalling during Drosophila wing developm...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2016-06-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/13301 |
| _version_ | 1811180617446981632 |
|---|---|
| author | Mark Norman Robin Vuilleumier Alexander Springhorn Jennifer Gawlik George Pyrowolakis |
| author_facet | Mark Norman Robin Vuilleumier Alexander Springhorn Jennifer Gawlik George Pyrowolakis |
| author_sort | Mark Norman |
| collection | DOAJ |
| description | Tight regulation of signalling activity is crucial for proper tissue patterning and growth. Here we investigate the function of Pentagone (Pent), a secreted protein that acts in a regulatory feedback during establishment and maintenance of BMP/Dpp morphogen signalling during Drosophila wing development. We show that Pent internalises the Dpp co-receptors, the glypicans Dally and Dally-like protein (Dlp), and propose that this internalisation is important in the establishment of a long range Dpp gradient. Pent-induced endocytosis and degradation of glypicans requires dynamin- and Rab5, but not clathrin or active BMP signalling. Thus, Pent modifies the ability of cells to trap and transduce BMP by fine-tuning the levels of the BMP reception system at the plasma membrane. In addition, and in accordance with the role of glypicans in multiple signalling pathways, we establish a requirement of Pent for Wg signalling. Our data propose a novel mechanism by which morphogen signalling is regulated. |
| first_indexed | 2024-04-11T09:06:16Z |
| format | Article |
| id | doaj.art-7d2bc2d31c004b9b951debbc3f4f9f3e |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:06:16Z |
| publishDate | 2016-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-7d2bc2d31c004b9b951debbc3f4f9f3e2022-12-22T04:32:37ZengeLife Sciences Publications LtdeLife2050-084X2016-06-01510.7554/eLife.13301Pentagone internalises glypicans to fine-tune multiple signalling pathwaysMark Norman0Robin Vuilleumier1Alexander Springhorn2Jennifer Gawlik3George Pyrowolakis4https://orcid.org/0000-0002-2142-5943Centre for Biological Signalling Studies, Albert-Ludwigs-University of Freiburg, Breisgau, GermanyInstitute for Biology I, Albert-Ludwigs-University of Freiburg, Breisgau, GermanyInstitute for Biology I, Albert-Ludwigs-University of Freiburg, Breisgau, Germany; Spemann Graduate School of Biology and Medicine, Albert-Ludwigs-University of Freiburg, Breisgau, GermanyCentre for Biological Signalling Studies, Albert-Ludwigs-University of Freiburg, Breisgau, Germany; Spemann Graduate School of Biology and Medicine, Albert-Ludwigs-University of Freiburg, Breisgau, GermanyCentre for Biological Signalling Studies, Albert-Ludwigs-University of Freiburg, Breisgau, Germany; Institute for Biology I, Albert-Ludwigs-University of Freiburg, Breisgau, GermanyTight regulation of signalling activity is crucial for proper tissue patterning and growth. Here we investigate the function of Pentagone (Pent), a secreted protein that acts in a regulatory feedback during establishment and maintenance of BMP/Dpp morphogen signalling during Drosophila wing development. We show that Pent internalises the Dpp co-receptors, the glypicans Dally and Dally-like protein (Dlp), and propose that this internalisation is important in the establishment of a long range Dpp gradient. Pent-induced endocytosis and degradation of glypicans requires dynamin- and Rab5, but not clathrin or active BMP signalling. Thus, Pent modifies the ability of cells to trap and transduce BMP by fine-tuning the levels of the BMP reception system at the plasma membrane. In addition, and in accordance with the role of glypicans in multiple signalling pathways, we establish a requirement of Pent for Wg signalling. Our data propose a novel mechanism by which morphogen signalling is regulated.https://elifesciences.org/articles/13301decapentaplegicmorphogenglypicansendocytosis |
| spellingShingle | Mark Norman Robin Vuilleumier Alexander Springhorn Jennifer Gawlik George Pyrowolakis Pentagone internalises glypicans to fine-tune multiple signalling pathways eLife decapentaplegic morphogen glypicans endocytosis |
| title | Pentagone internalises glypicans to fine-tune multiple signalling pathways |
| title_full | Pentagone internalises glypicans to fine-tune multiple signalling pathways |
| title_fullStr | Pentagone internalises glypicans to fine-tune multiple signalling pathways |
| title_full_unstemmed | Pentagone internalises glypicans to fine-tune multiple signalling pathways |
| title_short | Pentagone internalises glypicans to fine-tune multiple signalling pathways |
| title_sort | pentagone internalises glypicans to fine tune multiple signalling pathways |
| topic | decapentaplegic morphogen glypicans endocytosis |
| url | https://elifesciences.org/articles/13301 |
| work_keys_str_mv | AT marknorman pentagoneinternalisesglypicanstofinetunemultiplesignallingpathways AT robinvuilleumier pentagoneinternalisesglypicanstofinetunemultiplesignallingpathways AT alexanderspringhorn pentagoneinternalisesglypicanstofinetunemultiplesignallingpathways AT jennifergawlik pentagoneinternalisesglypicanstofinetunemultiplesignallingpathways AT georgepyrowolakis pentagoneinternalisesglypicanstofinetunemultiplesignallingpathways |