Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoides
Micropterus salmoides is one of the most economically valuable fish species in China. Currently, information in the effect of probiotics-supplemented feed on M. salmoides is rare. Present study investigated the impact of supplementary Lactobacillus casei K17 with six treatments on growth performance...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513421001502 |
| _version_ | 1818456162882813952 |
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| author | Jinsong Wang Zhuoying Zhu Rui Li Xichang Wang Xiangjun Leng Lanming Chen |
| author_facet | Jinsong Wang Zhuoying Zhu Rui Li Xichang Wang Xiangjun Leng Lanming Chen |
| author_sort | Jinsong Wang |
| collection | DOAJ |
| description | Micropterus salmoides is one of the most economically valuable fish species in China. Currently, information in the effect of probiotics-supplemented feed on M. salmoides is rare. Present study investigated the impact of supplementary Lactobacillus casei K17 with six treatments on growth performance, gut digestion, absorption and non-specific immune, and gut bacterial biota and metabolomics of M. salmoides. A total of 450 M. salmoides (114 day of age, average 33.0 ± 0.5 g) were randomly allocated into six groups for a 69-day rearing experiments. M. salmoides was given commercial feed supplemented with: 1) live L. casei K17 (1 × 109 CFU/g), 2) live L. casei K17 protected by skim milk powder (10 %), 3) heat-killed dead L. casei K17, 4) its fermentation supernatant, 5) skim milk powder (10 %), and 6) normal saline (0.85 % NaCl), designated as LB, MB, DB, FS, MG, and SG, respectively. MB and DB diets significantly increased body weight and growth rate, but decreased feed conversion ratio of M. salmoides (p < 0.05). These two diets also significantly increased gut amylase and lipase activities and microvilli surface areas (p < 0.05). Comparative bacterial biota analysis revealed distinct bacterial community compositions in M. salmoides gut contents among the six groups, which were remarkably dominated by L. casei in LB (90.0 %), MB (95.5 %), and DB (92.4 %) groups. Occurrences of some pathogenic bacteria were significantly reduced (p < 0.05). Meanwhile, up-modulated carbohydrate and down-modulated amino acid metabolisms were observed in LB, MB, and DB groups (p < 0.05). Comparative metabolomic analysis also revealed a number of notably altered metabolites, which, coupled with increased serum LZM and SOD, improved gut non-specific immune of M. salmoides, particularly in MB and DB groups. The results provided a reference of promising probiotic component L. casei K17 to feed industry. |
| first_indexed | 2024-12-14T22:22:18Z |
| format | Article |
| id | doaj.art-7d2e18290cb34098b616c04d39cde232 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-14T22:22:18Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj.art-7d2e18290cb34098b616c04d39cde2322022-12-21T22:45:28ZengElsevierAquaculture Reports2352-51342021-07-0120100734Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoidesJinsong Wang0Zhuoying Zhu1Rui Li2Xichang Wang3Xiangjun Leng4Lanming Chen5Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People's Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; College of Bioengineering, Jingchu University of Technology, Jingmen 448000, ChinaKey Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People's Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Bioengineering, Jingchu University of Technology, Jingmen 448000, ChinaKey Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People's Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People's Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Corresponding author.Micropterus salmoides is one of the most economically valuable fish species in China. Currently, information in the effect of probiotics-supplemented feed on M. salmoides is rare. Present study investigated the impact of supplementary Lactobacillus casei K17 with six treatments on growth performance, gut digestion, absorption and non-specific immune, and gut bacterial biota and metabolomics of M. salmoides. A total of 450 M. salmoides (114 day of age, average 33.0 ± 0.5 g) were randomly allocated into six groups for a 69-day rearing experiments. M. salmoides was given commercial feed supplemented with: 1) live L. casei K17 (1 × 109 CFU/g), 2) live L. casei K17 protected by skim milk powder (10 %), 3) heat-killed dead L. casei K17, 4) its fermentation supernatant, 5) skim milk powder (10 %), and 6) normal saline (0.85 % NaCl), designated as LB, MB, DB, FS, MG, and SG, respectively. MB and DB diets significantly increased body weight and growth rate, but decreased feed conversion ratio of M. salmoides (p < 0.05). These two diets also significantly increased gut amylase and lipase activities and microvilli surface areas (p < 0.05). Comparative bacterial biota analysis revealed distinct bacterial community compositions in M. salmoides gut contents among the six groups, which were remarkably dominated by L. casei in LB (90.0 %), MB (95.5 %), and DB (92.4 %) groups. Occurrences of some pathogenic bacteria were significantly reduced (p < 0.05). Meanwhile, up-modulated carbohydrate and down-modulated amino acid metabolisms were observed in LB, MB, and DB groups (p < 0.05). Comparative metabolomic analysis also revealed a number of notably altered metabolites, which, coupled with increased serum LZM and SOD, improved gut non-specific immune of M. salmoides, particularly in MB and DB groups. The results provided a reference of promising probiotic component L. casei K17 to feed industry.http://www.sciencedirect.com/science/article/pii/S2352513421001502Lactobacillus caseiMicropterus salmoidesGrowth performanceGut healthBacterial biotaMetabolomics |
| spellingShingle | Jinsong Wang Zhuoying Zhu Rui Li Xichang Wang Xiangjun Leng Lanming Chen Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoides Aquaculture Reports Lactobacillus casei Micropterus salmoides Growth performance Gut health Bacterial biota Metabolomics |
| title | Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoides |
| title_full | Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoides |
| title_fullStr | Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoides |
| title_full_unstemmed | Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoides |
| title_short | Impact of supplementary Lactobacillus casei K17 on growth and gut health of largemouth bass Micropterus salmoides |
| title_sort | impact of supplementary lactobacillus casei k17 on growth and gut health of largemouth bass micropterus salmoides |
| topic | Lactobacillus casei Micropterus salmoides Growth performance Gut health Bacterial biota Metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S2352513421001502 |
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