Antigen-specific downregulation of miR-150 in CD4 T cells promotes cell survival
MicroRNA-150 (miR-150) has been shown to play a general role in the immune system, but very little is known about its role on CD4+ T cell responses. During T cell responses against superantigen Staphylococcal Enterotoxin A, miR-150 expression was down-regulated in antigen-specific CD4+ T cells but u...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1102403/full |
| _version_ | 1811173099460100096 |
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| author | Antoine Ménoret Federica Agliano Timofey A. Karginov Keaton S. Karlinsey Beiyan Zhou Anthony T. Vella |
| author_facet | Antoine Ménoret Federica Agliano Timofey A. Karginov Keaton S. Karlinsey Beiyan Zhou Anthony T. Vella |
| author_sort | Antoine Ménoret |
| collection | DOAJ |
| description | MicroRNA-150 (miR-150) has been shown to play a general role in the immune system, but very little is known about its role on CD4+ T cell responses. During T cell responses against superantigen Staphylococcal Enterotoxin A, miR-150 expression was down-regulated in antigen-specific CD4+ T cells but up-regulated in CD8+ T cells. CD4+ and CD8+ T cell clonal expansion was greater in miR-150-KO mice than in WT mice, but miR-150 selectively repressed IL-2 production in CD4+ T cells. Transcriptome analysis of CD4+ T cells demonstrated that apoptosis and mTOR pathways were highly enriched in the absence of miR-150. Mechanistic studies confirmed that miR-150 promoted apoptosis specifically in antigen-specific CD4+ T cells, but not in bystander CD4+ nor in CD8+ T cells. Furthermore, inhibition of mTOR-linked mitochondrial superoxidedismutase-2 increased apoptosis in miR-150-/- antigen-specific CD4+ T. Thus, miR-150 impacts CD4+ T cell helper activity by attenuating IL-2 production along with clonal expansion, and suppresses superoxidedismutase to promote apoptosis. |
| first_indexed | 2024-04-10T17:42:14Z |
| format | Article |
| id | doaj.art-7d33ae2362004c1e8e8efe8f3aca50db |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T17:42:14Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-7d33ae2362004c1e8e8efe8f3aca50db2023-02-03T11:50:43ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-01-011410.3389/fimmu.2023.11024031102403Antigen-specific downregulation of miR-150 in CD4 T cells promotes cell survivalAntoine MénoretFederica AglianoTimofey A. KarginovKeaton S. KarlinseyBeiyan ZhouAnthony T. VellaMicroRNA-150 (miR-150) has been shown to play a general role in the immune system, but very little is known about its role on CD4+ T cell responses. During T cell responses against superantigen Staphylococcal Enterotoxin A, miR-150 expression was down-regulated in antigen-specific CD4+ T cells but up-regulated in CD8+ T cells. CD4+ and CD8+ T cell clonal expansion was greater in miR-150-KO mice than in WT mice, but miR-150 selectively repressed IL-2 production in CD4+ T cells. Transcriptome analysis of CD4+ T cells demonstrated that apoptosis and mTOR pathways were highly enriched in the absence of miR-150. Mechanistic studies confirmed that miR-150 promoted apoptosis specifically in antigen-specific CD4+ T cells, but not in bystander CD4+ nor in CD8+ T cells. Furthermore, inhibition of mTOR-linked mitochondrial superoxidedismutase-2 increased apoptosis in miR-150-/- antigen-specific CD4+ T. Thus, miR-150 impacts CD4+ T cell helper activity by attenuating IL-2 production along with clonal expansion, and suppresses superoxidedismutase to promote apoptosis.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1102403/fullMiR-150lymphocytenon-coding RNAsuperantigenmTORapoptosis |
| spellingShingle | Antoine Ménoret Federica Agliano Timofey A. Karginov Keaton S. Karlinsey Beiyan Zhou Anthony T. Vella Antigen-specific downregulation of miR-150 in CD4 T cells promotes cell survival Frontiers in Immunology MiR-150 lymphocyte non-coding RNA superantigen mTOR apoptosis |
| title | Antigen-specific downregulation of miR-150 in CD4 T cells promotes cell survival |
| title_full | Antigen-specific downregulation of miR-150 in CD4 T cells promotes cell survival |
| title_fullStr | Antigen-specific downregulation of miR-150 in CD4 T cells promotes cell survival |
| title_full_unstemmed | Antigen-specific downregulation of miR-150 in CD4 T cells promotes cell survival |
| title_short | Antigen-specific downregulation of miR-150 in CD4 T cells promotes cell survival |
| title_sort | antigen specific downregulation of mir 150 in cd4 t cells promotes cell survival |
| topic | MiR-150 lymphocyte non-coding RNA superantigen mTOR apoptosis |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1102403/full |
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