In vitro antifungal activity of chitosan on Bipolaris oryzae, rice pathogen

The aim of this study was to determine the effect and Media Inhibitory Concentration (MIC50), of chitosans of different molecular masses on mycelial growth, hyphal diameter, structural changes, spore germination and fungal biomass production B. oryzae. The results indicated that the average molecula...

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Main Authors: Aida Tania Rodríguez Pedroso, Maribel Plascencia Jatomea, Silvia Bautista Baños, Mario Onofre Cortéz Rocha, Miguel Ángel Ramírez Arrebato
Format: Article
Language:English
Published: Universidad Nacional de Colombia 2016-01-01
Series:Acta Agronómica
Subjects:
Online Access:https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/48235
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author Aida Tania Rodríguez Pedroso
Maribel Plascencia Jatomea
Silvia Bautista Baños
Mario Onofre Cortéz Rocha
Miguel Ángel Ramírez Arrebato
author_facet Aida Tania Rodríguez Pedroso
Maribel Plascencia Jatomea
Silvia Bautista Baños
Mario Onofre Cortéz Rocha
Miguel Ángel Ramírez Arrebato
author_sort Aida Tania Rodríguez Pedroso
collection DOAJ
description The aim of this study was to determine the effect and Media Inhibitory Concentration (MIC50), of chitosans of different molecular masses on mycelial growth, hyphal diameter, structural changes, spore germination and fungal biomass production B. oryzae. The results indicated that the average molecular mass chitosan showed higher inhibitory effect (p≤0.05); however, the low molecular weight chitosan affected more hyphal diameter at the highest concentration used (4000 mg/l) Observations of hyphae through the scanning electron microscopy (SEM) showed that the fungus treated with chitosan at the concentration of 3000 mg/l achieved a high spore production. The average molecular mass chitosan had the lowest MIC value was 2182.9 mg/l. The germination of spores treated with CMI50 was affected and biomass production of the fungus.
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spelling doaj.art-7d33e48bde3946869f423d54d936d22b2022-12-22T03:14:52ZengUniversidad Nacional de ColombiaActa Agronómica0120-28122323-01182016-01-016519810310.15446/acag.v65n1.4823541291In vitro antifungal activity of chitosan on Bipolaris oryzae, rice pathogenAida Tania Rodríguez Pedroso0Maribel Plascencia Jatomea1Silvia Bautista Baños2Mario Onofre Cortéz Rocha3Miguel Ángel Ramírez Arrebato4Instituto Nacional de Ciencias Agrícolas (INCA), CubaUniversidad de Sonora, Departamento de Investigaciones y Posgrado en Alimento (DIPA), MéxicoInstituto Politécnico Nacional-CEPROBI, MéxicoUniversidad de Sonora, Departamento de Investigaciones y Posgrado en Alimento (DIPA), MéxicoInstituto Nacional de Ciencias Agrícolas (INCA), CubaThe aim of this study was to determine the effect and Media Inhibitory Concentration (MIC50), of chitosans of different molecular masses on mycelial growth, hyphal diameter, structural changes, spore germination and fungal biomass production B. oryzae. The results indicated that the average molecular mass chitosan showed higher inhibitory effect (p≤0.05); however, the low molecular weight chitosan affected more hyphal diameter at the highest concentration used (4000 mg/l) Observations of hyphae through the scanning electron microscopy (SEM) showed that the fungus treated with chitosan at the concentration of 3000 mg/l achieved a high spore production. The average molecular mass chitosan had the lowest MIC value was 2182.9 mg/l. The germination of spores treated with CMI50 was affected and biomass production of the fungus.https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/48235BiomasaesporasCMI50SEM
spellingShingle Aida Tania Rodríguez Pedroso
Maribel Plascencia Jatomea
Silvia Bautista Baños
Mario Onofre Cortéz Rocha
Miguel Ángel Ramírez Arrebato
In vitro antifungal activity of chitosan on Bipolaris oryzae, rice pathogen
Acta Agronómica
Biomasa
esporas
CMI50
SEM
title In vitro antifungal activity of chitosan on Bipolaris oryzae, rice pathogen
title_full In vitro antifungal activity of chitosan on Bipolaris oryzae, rice pathogen
title_fullStr In vitro antifungal activity of chitosan on Bipolaris oryzae, rice pathogen
title_full_unstemmed In vitro antifungal activity of chitosan on Bipolaris oryzae, rice pathogen
title_short In vitro antifungal activity of chitosan on Bipolaris oryzae, rice pathogen
title_sort in vitro antifungal activity of chitosan on bipolaris oryzae rice pathogen
topic Biomasa
esporas
CMI50
SEM
url https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/48235
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