Development of a Novel Hanging Drop Platform for Engineering Controllable 3D Microenvironments
Conventional biomedical research is mostly performed by utilizing a two-dimensional monolayer culture, which fails to recapitulate the three-dimensional (3D) organization and microenvironment of native tissues. To overcome this limitation, several methods are developed to fabricate microtissues with...
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| Format: | Article |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00327/full |
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| author | Chin-Yi Cho Tzu-Hsiang Chiang Li-Hung Hsieh Wen-Yu Yang Wen-Yu Yang Hsiang-Hao Hsu Hsiang-Hao Hsu Chih-Kuang Yeh Chieh-Cheng Huang Jen-Huang Huang |
| author_facet | Chin-Yi Cho Tzu-Hsiang Chiang Li-Hung Hsieh Wen-Yu Yang Wen-Yu Yang Hsiang-Hao Hsu Hsiang-Hao Hsu Chih-Kuang Yeh Chieh-Cheng Huang Jen-Huang Huang |
| author_sort | Chin-Yi Cho |
| collection | DOAJ |
| description | Conventional biomedical research is mostly performed by utilizing a two-dimensional monolayer culture, which fails to recapitulate the three-dimensional (3D) organization and microenvironment of native tissues. To overcome this limitation, several methods are developed to fabricate microtissues with the desired 3D microenvironment. However, they tend to be time-consuming, labor-intensive, or costly, thus hindering the application of 3D microtissues as models in a wide variety of research fields. In the present study, we have developed a pressure-assisted network for droplet accumulation (PANDA) system, an easy-to-use chip that comprises a multichannel fluidic system and a hanging drop cell culture module for uniform 3D microtissue formation. This system can control the desired artificial niches for modulating the fate of the stem cells to form the different sizes of microtissue by adjusting the seeding density. Furthermore, a large number of highly consistent 3D glomerulus-like heterogeneous microtissues that are composed of kidney glomerular podocytes and mesenchymal stem cells have been formed successfully. These data suggest that the developed PANDA system can be employed as a rapid and economical platform to fabricate microtissues with tunable 3D microenvironment and cellular heterogeneity, thus can be employed as tissue-mimicking models in various biomedical research. |
| first_indexed | 2024-12-10T15:11:23Z |
| format | Article |
| id | doaj.art-7d380129e57e426095550b6b894160dc |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-10T15:11:23Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-7d380129e57e426095550b6b894160dc2022-12-22T01:43:55ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-05-01810.3389/fcell.2020.00327538033Development of a Novel Hanging Drop Platform for Engineering Controllable 3D MicroenvironmentsChin-Yi Cho0Tzu-Hsiang Chiang1Li-Hung Hsieh2Wen-Yu Yang3Wen-Yu Yang4Hsiang-Hao Hsu5Hsiang-Hao Hsu6Chih-Kuang Yeh7Chieh-Cheng Huang8Jen-Huang Huang9Department of Chemical Engineering, National Tsing Hua University, Hsinchu, TaiwanDepartment of Chemical Engineering, National Tsing Hua University, Hsinchu, TaiwanInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu, TaiwanInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu, TaiwanDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, TaiwanDepartment of Nephrology, Kidney Research Center, Linkou Chang Gung Memorial Hospital, Taoyuan, TaiwanCollege of Medicine, School of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, TaiwanInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu, TaiwanDepartment of Chemical Engineering, National Tsing Hua University, Hsinchu, TaiwanConventional biomedical research is mostly performed by utilizing a two-dimensional monolayer culture, which fails to recapitulate the three-dimensional (3D) organization and microenvironment of native tissues. To overcome this limitation, several methods are developed to fabricate microtissues with the desired 3D microenvironment. However, they tend to be time-consuming, labor-intensive, or costly, thus hindering the application of 3D microtissues as models in a wide variety of research fields. In the present study, we have developed a pressure-assisted network for droplet accumulation (PANDA) system, an easy-to-use chip that comprises a multichannel fluidic system and a hanging drop cell culture module for uniform 3D microtissue formation. This system can control the desired artificial niches for modulating the fate of the stem cells to form the different sizes of microtissue by adjusting the seeding density. Furthermore, a large number of highly consistent 3D glomerulus-like heterogeneous microtissues that are composed of kidney glomerular podocytes and mesenchymal stem cells have been formed successfully. These data suggest that the developed PANDA system can be employed as a rapid and economical platform to fabricate microtissues with tunable 3D microenvironment and cellular heterogeneity, thus can be employed as tissue-mimicking models in various biomedical research.https://www.frontiersin.org/article/10.3389/fcell.2020.00327/fullhanging droparraymicrotissueglomeruluspodocyte |
| spellingShingle | Chin-Yi Cho Tzu-Hsiang Chiang Li-Hung Hsieh Wen-Yu Yang Wen-Yu Yang Hsiang-Hao Hsu Hsiang-Hao Hsu Chih-Kuang Yeh Chieh-Cheng Huang Jen-Huang Huang Development of a Novel Hanging Drop Platform for Engineering Controllable 3D Microenvironments Frontiers in Cell and Developmental Biology hanging drop array microtissue glomerulus podocyte |
| title | Development of a Novel Hanging Drop Platform for Engineering Controllable 3D Microenvironments |
| title_full | Development of a Novel Hanging Drop Platform for Engineering Controllable 3D Microenvironments |
| title_fullStr | Development of a Novel Hanging Drop Platform for Engineering Controllable 3D Microenvironments |
| title_full_unstemmed | Development of a Novel Hanging Drop Platform for Engineering Controllable 3D Microenvironments |
| title_short | Development of a Novel Hanging Drop Platform for Engineering Controllable 3D Microenvironments |
| title_sort | development of a novel hanging drop platform for engineering controllable 3d microenvironments |
| topic | hanging drop array microtissue glomerulus podocyte |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00327/full |
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