Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity
When culturing cells in space or under altered gravity conditions on Earth to investigate the impact of gravity, their adhesion and organoid formation capabilities change. In search of a target where the alteration of gravity force could have this impact, we investigated p130cas/BCAR1 and its intera...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Computation |
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| Online Access: | https://www.mdpi.com/2079-3197/9/8/81 |
| _version_ | 1797524249195315200 |
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| author | Johann Bauer Erich Gombocz Herbert Schulz Jens Hauslage Daniela Grimm |
| author_facet | Johann Bauer Erich Gombocz Herbert Schulz Jens Hauslage Daniela Grimm |
| author_sort | Johann Bauer |
| collection | DOAJ |
| description | When culturing cells in space or under altered gravity conditions on Earth to investigate the impact of gravity, their adhesion and organoid formation capabilities change. In search of a target where the alteration of gravity force could have this impact, we investigated p130cas/BCAR1 and its interactions more thoroughly, particularly as its activity is sensitive to applied forces. This protein is well characterized regarding its role in growth stimulation and adhesion processes. To better understand BCAR1′s force-dependent scaffolding of other proteins, we studied its interactions with proteins we had detected by proteome analyses of MCF-7 breast cancer and FTC-133 thyroid cancer cells, which are both sensitive to exposure to microgravity and express BCAR1. Using linked open data resources and our experiments, we collected comprehensive information to establish a semantic knowledgebase and analyzed identified proteins belonging to signaling pathways and their networks. The results show that the force-dependent phosphorylation and scaffolding of BCAR1 influence the structure, function, and degradation of intracellular proteins as well as the growth, adhesion and apoptosis of cells similarly to exposure of whole cells to altered gravity. As BCAR1 evidently plays a significant role in cell responses to gravity changes, this study reveals a clear path to future research performing phosphorylation experiments on BCAR1. |
| first_indexed | 2024-03-10T08:54:38Z |
| format | Article |
| id | doaj.art-7d3a3c9d644a43108a2807b8142a6c10 |
| institution | Directory Open Access Journal |
| issn | 2079-3197 |
| language | English |
| last_indexed | 2024-03-10T08:54:38Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Computation |
| spelling | doaj.art-7d3a3c9d644a43108a2807b8142a6c102023-11-22T07:15:20ZengMDPI AGComputation2079-31972021-07-01988110.3390/computation9080081Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in GravityJohann Bauer0Erich Gombocz1Herbert Schulz2Jens Hauslage3Daniela Grimm4SiHaTho GmbH, Postfach 1106, 35201 Biedenkopf, GermanyMelissa Informatics, 22382 Avenida Empresa, Rancho Sana Margarita, CA 92688, USADepartment of Microgravity and Translational Regenerative Medicine, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University, 39106 Magdeburg, GermanyDLR, Institut für Luft- und Raumfahrtmedizin, Linder Höhe, 51147 Köln, GermanyDepartment of Microgravity and Translational Regenerative Medicine, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University, 39106 Magdeburg, GermanyWhen culturing cells in space or under altered gravity conditions on Earth to investigate the impact of gravity, their adhesion and organoid formation capabilities change. In search of a target where the alteration of gravity force could have this impact, we investigated p130cas/BCAR1 and its interactions more thoroughly, particularly as its activity is sensitive to applied forces. This protein is well characterized regarding its role in growth stimulation and adhesion processes. To better understand BCAR1′s force-dependent scaffolding of other proteins, we studied its interactions with proteins we had detected by proteome analyses of MCF-7 breast cancer and FTC-133 thyroid cancer cells, which are both sensitive to exposure to microgravity and express BCAR1. Using linked open data resources and our experiments, we collected comprehensive information to establish a semantic knowledgebase and analyzed identified proteins belonging to signaling pathways and their networks. The results show that the force-dependent phosphorylation and scaffolding of BCAR1 influence the structure, function, and degradation of intracellular proteins as well as the growth, adhesion and apoptosis of cells similarly to exposure of whole cells to altered gravity. As BCAR1 evidently plays a significant role in cell responses to gravity changes, this study reveals a clear path to future research performing phosphorylation experiments on BCAR1.https://www.mdpi.com/2079-3197/9/8/81knowledge explorerpathway studiomass spectrometrypost-translational modificationnetwork analysisSPARQL |
| spellingShingle | Johann Bauer Erich Gombocz Herbert Schulz Jens Hauslage Daniela Grimm Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity Computation knowledge explorer pathway studio mass spectrometry post-translational modification network analysis SPARQL |
| title | Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity |
| title_full | Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity |
| title_fullStr | Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity |
| title_full_unstemmed | Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity |
| title_short | Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity |
| title_sort | interaction network provides clues on the role of bcar1 in cellular response to changes in gravity |
| topic | knowledge explorer pathway studio mass spectrometry post-translational modification network analysis SPARQL |
| url | https://www.mdpi.com/2079-3197/9/8/81 |
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