Intein-Mediated Protein Engineering for Cell-Based Biosensors
Cell-based sensors provide a flexible platform for screening biologically active targets and for monitoring their interactions in live cells. Their applicability extends across a vast array of biological research and clinical applications. Particularly, cell-based sensors are becoming a potent tool...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/5/283 |
| _version_ | 1827670049123467264 |
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| author | Chungwon Kang Keshab Lal Shrestha San Kwon Seungil Park Jinsik Kim Youngeun Kwon |
| author_facet | Chungwon Kang Keshab Lal Shrestha San Kwon Seungil Park Jinsik Kim Youngeun Kwon |
| author_sort | Chungwon Kang |
| collection | DOAJ |
| description | Cell-based sensors provide a flexible platform for screening biologically active targets and for monitoring their interactions in live cells. Their applicability extends across a vast array of biological research and clinical applications. Particularly, cell-based sensors are becoming a potent tool in drug discovery and cell-signaling studies by allowing function-based screening of targets in biologically relevant environments and enabling the in vivo visualization of cellular signals in real-time with an outstanding spatiotemporal resolution. In this review, we aim to provide a clear view of current cell-based sensor technologies, their limitations, and how the recent improvements were using intein-mediated protein engineering. We first discuss the characteristics of cell-based sensors and present several representative examples with a focus on their design strategies, which differentiate cell-based sensors from in vitro analytical biosensors. We then describe the application of intein-mediated protein engineering technology for cell-based sensor fabrication. Finally, we explain the characteristics of intein-mediated reactions and present examples of how the intein-mediated reactions are used to improve existing methods and develop new approaches in sensor cell fabrication to address the limitations of current technologies. |
| first_indexed | 2024-03-10T03:15:39Z |
| format | Article |
| id | doaj.art-7d3ba6817e01448eaa28f53f66c4f8d7 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T03:15:39Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-7d3ba6817e01448eaa28f53f66c4f8d72023-11-23T10:15:15ZengMDPI AGBiosensors2079-63742022-04-0112528310.3390/bios12050283Intein-Mediated Protein Engineering for Cell-Based BiosensorsChungwon Kang0Keshab Lal Shrestha1San Kwon2Seungil Park3Jinsik Kim4Youngeun Kwon5Department of Biomedical Engineering, Dongguk University, Seoul 04260, KoreaDepartment of Biomedical Engineering, Dongguk University, Seoul 04260, KoreaDepartment of Biomedical Engineering, Dongguk University, Seoul 04260, KoreaDepartment of Biomedical Engineering, Dongguk University, Seoul 04260, KoreaDepartment of Biomedical Engineering, Dongguk University, Seoul 04260, KoreaDepartment of Biomedical Engineering, Dongguk University, Seoul 04260, KoreaCell-based sensors provide a flexible platform for screening biologically active targets and for monitoring their interactions in live cells. Their applicability extends across a vast array of biological research and clinical applications. Particularly, cell-based sensors are becoming a potent tool in drug discovery and cell-signaling studies by allowing function-based screening of targets in biologically relevant environments and enabling the in vivo visualization of cellular signals in real-time with an outstanding spatiotemporal resolution. In this review, we aim to provide a clear view of current cell-based sensor technologies, their limitations, and how the recent improvements were using intein-mediated protein engineering. We first discuss the characteristics of cell-based sensors and present several representative examples with a focus on their design strategies, which differentiate cell-based sensors from in vitro analytical biosensors. We then describe the application of intein-mediated protein engineering technology for cell-based sensor fabrication. Finally, we explain the characteristics of intein-mediated reactions and present examples of how the intein-mediated reactions are used to improve existing methods and develop new approaches in sensor cell fabrication to address the limitations of current technologies.https://www.mdpi.com/2079-6374/12/5/283biosensorscell-based sensorsbio-recognition elementreporter elementsplit-inteinconditional protein splicing |
| spellingShingle | Chungwon Kang Keshab Lal Shrestha San Kwon Seungil Park Jinsik Kim Youngeun Kwon Intein-Mediated Protein Engineering for Cell-Based Biosensors Biosensors biosensors cell-based sensors bio-recognition element reporter element split-intein conditional protein splicing |
| title | Intein-Mediated Protein Engineering for Cell-Based Biosensors |
| title_full | Intein-Mediated Protein Engineering for Cell-Based Biosensors |
| title_fullStr | Intein-Mediated Protein Engineering for Cell-Based Biosensors |
| title_full_unstemmed | Intein-Mediated Protein Engineering for Cell-Based Biosensors |
| title_short | Intein-Mediated Protein Engineering for Cell-Based Biosensors |
| title_sort | intein mediated protein engineering for cell based biosensors |
| topic | biosensors cell-based sensors bio-recognition element reporter element split-intein conditional protein splicing |
| url | https://www.mdpi.com/2079-6374/12/5/283 |
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