Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China

Introduction: A real-time RT-PCR method for identification and quantification of porcine epidemic diarrhoea virus (PEDV) strains in China was developed.

Bibliographic Details
Main Authors: Hou Yi-Xuan, Xie Chun, Wang Kang, Zhao Yu-Ting, Xie Yang-Yang, Shi Hong-Yan, Chen Jian-Fei, Feng Li, Tong Guang-Zhi, Hua Xiu-Guo, Yuan Cong-Li, Zhou Yan-Jun, Yang Zhi-Biao
Format: Article
Language:English
Published: Sciendo 2016-06-01
Series:Journal of Veterinary Research
Subjects:
Online Access:https://doi.org/10.1515/jvetres-2016-0018
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author Hou Yi-Xuan
Xie Chun
Wang Kang
Zhao Yu-Ting
Xie Yang-Yang
Shi Hong-Yan
Chen Jian-Fei
Feng Li
Tong Guang-Zhi
Hua Xiu-Guo
Yuan Cong-Li
Zhou Yan-Jun
Yang Zhi-Biao
author_facet Hou Yi-Xuan
Xie Chun
Wang Kang
Zhao Yu-Ting
Xie Yang-Yang
Shi Hong-Yan
Chen Jian-Fei
Feng Li
Tong Guang-Zhi
Hua Xiu-Guo
Yuan Cong-Li
Zhou Yan-Jun
Yang Zhi-Biao
author_sort Hou Yi-Xuan
collection DOAJ
description Introduction: A real-time RT-PCR method for identification and quantification of porcine epidemic diarrhoea virus (PEDV) strains in China was developed.
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spelling doaj.art-7d3d152f7a094123b0da04923aa4d4762022-12-21T22:07:38ZengSciendoJournal of Veterinary Research2450-86082016-06-0160212713310.1515/jvetres-2016-0018jvetres-2016-0018Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in ChinaHou Yi-Xuan0Xie Chun1Wang Kang2Zhao Yu-Ting3Xie Yang-Yang4Shi Hong-Yan5Chen Jian-Fei6Feng Li7Tong Guang-Zhi8Hua Xiu-Guo9Yuan Cong-Li10Zhou Yan-Jun11Yang Zhi-Biao12Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of ChinaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of ChinaDivision of Swine Infectious Diseases, Shanghai Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Shanghai 200241, People’s Republic of ChinaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of ChinaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of ChinaDivision of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, People’s Republic of ChinaDivision of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, People’s Republic of ChinaDivision of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, People’s Republic of ChinaDivision of Swine Infectious Diseases, Shanghai Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Shanghai 200241, People’s Republic of ChinaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of ChinaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of ChinaDivision of Swine Infectious Diseases, Shanghai Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Shanghai 200241, People’s Republic of ChinaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of ChinaIntroduction: A real-time RT-PCR method for identification and quantification of porcine epidemic diarrhoea virus (PEDV) strains in China was developed.https://doi.org/10.1515/jvetres-2016-0018porcine epidemic diarrhoea virustaqman-mgb real-time rt-pcrpeople’s republic of china
spellingShingle Hou Yi-Xuan
Xie Chun
Wang Kang
Zhao Yu-Ting
Xie Yang-Yang
Shi Hong-Yan
Chen Jian-Fei
Feng Li
Tong Guang-Zhi
Hua Xiu-Guo
Yuan Cong-Li
Zhou Yan-Jun
Yang Zhi-Biao
Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China
Journal of Veterinary Research
porcine epidemic diarrhoea virus
taqman-mgb real-time rt-pcr
people’s republic of china
title Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China
title_full Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China
title_fullStr Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China
title_full_unstemmed Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China
title_short Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China
title_sort development and application of a taqman mgb real time rt pcr assay for the detection of porcine epidemic diarrhoea virus strains in china
topic porcine epidemic diarrhoea virus
taqman-mgb real-time rt-pcr
people’s republic of china
url https://doi.org/10.1515/jvetres-2016-0018
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