Identification of Staphylococcus aureus in Synovial Fluid of Patients Suspected to Arthritis Through PCR in Urmia City
Objective: Synovial fluid is composed of plasma ultrafiltration and hyaluronic acid secretion by synovial cells. Synovial fluid plays a role as softener and feeding cartilages without vessels. Infectious arthritis is one of the commonest arthritis and if the disease did not cure in the first days it...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Aras Part Medical International Press
2016-10-01
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| Series: | Crescent Journal of Medical and Biological Sciences |
| Subjects: | |
| Online Access: | http://www.cjmb.org/uploads/pdf/pdf_CJMB_23.pdf |
| _version_ | 1818690325119500288 |
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| author | Afshin Taravati Afshin Zahedi Hiro Allipashaie Mehdi Dilmagani |
| author_facet | Afshin Taravati Afshin Zahedi Hiro Allipashaie Mehdi Dilmagani |
| author_sort | Afshin Taravati |
| collection | DOAJ |
| description | Objective: Synovial fluid is composed of plasma ultrafiltration and hyaluronic acid secretion by synovial cells. Synovial fluid plays a role as softener and feeding cartilages without vessels. Infectious arthritis is one of the commonest arthritis and if the disease did not cure in the first days it would injure cartilages irreversibly. The goal of this study was identification of Staphylococcus aureus in synovial fluid of patients suspected to arthritis through PCR in Urmia city.
Materials and Methods: In this research synovial fluid contamination with Staphylococcus aureus and biochemical parameters such as the amount of glucose, protein and the number of white blood cells were studied. Amplification of nuc gene with the length of 279 bp using PCR method was applied to confirm Staphylococcus aureus isolation.
Results: For this, 400 cerebrospinal fluid samples were tested from hospitalized patients with arthritis in two hospitals in Urmia city during 3 months, which out of them 109 of samples were contaminated with bacteria including: 78 of isolates were Staphylococcus aureus, 12 of them were coagulase negative Staphylococci, 4 of them were Streptococcus and 15 of them were gram negative bacilli. Also, results showed that the amounts of glucose in positive samples in comparison to the amount of glucose in synovial fluid were significantly decreased. The amount of protein and the number of white blood cells in synovial fluid of positive samples were significantly higher in comparison to normal synovial fluid.
Conclusion: Results showed that Staphylococcus aureus is the most common agent at infections arthritis, therefore it is recommended to use an experimental treatment for Staphylococcus aureus prior to final results. |
| first_indexed | 2024-12-17T12:24:12Z |
| format | Article |
| id | doaj.art-7d3dae29607b4a9eae25f7ac4b2ae9de |
| institution | Directory Open Access Journal |
| issn | 2148-9696 2148-9696 |
| language | English |
| last_indexed | 2024-12-17T12:24:12Z |
| publishDate | 2016-10-01 |
| publisher | Aras Part Medical International Press |
| record_format | Article |
| series | Crescent Journal of Medical and Biological Sciences |
| spelling | doaj.art-7d3dae29607b4a9eae25f7ac4b2ae9de2022-12-21T21:48:50ZengAras Part Medical International PressCrescent Journal of Medical and Biological Sciences2148-96962148-96962016-10-0134128131Identification of Staphylococcus aureus in Synovial Fluid of Patients Suspected to Arthritis Through PCR in Urmia CityAfshin Taravati0Afshin Zahedi1 Hiro Allipashaie2Mehdi Dilmagani3Department of Veterinary Sciences, Rasht Branch, Islamic Azad University, Rasht, IranDepartment of Veterinary Sciences, Rasht Branch, Islamic Azad University, Rasht, IranDepartment of Biotechnology, Urmia Branch, Islamic Azad University, Urmia, IranDepartment of Cellular and Molecular Biology West Azerbaijan Veterinary Laboratory (WAVL), Urmia, IranObjective: Synovial fluid is composed of plasma ultrafiltration and hyaluronic acid secretion by synovial cells. Synovial fluid plays a role as softener and feeding cartilages without vessels. Infectious arthritis is one of the commonest arthritis and if the disease did not cure in the first days it would injure cartilages irreversibly. The goal of this study was identification of Staphylococcus aureus in synovial fluid of patients suspected to arthritis through PCR in Urmia city. Materials and Methods: In this research synovial fluid contamination with Staphylococcus aureus and biochemical parameters such as the amount of glucose, protein and the number of white blood cells were studied. Amplification of nuc gene with the length of 279 bp using PCR method was applied to confirm Staphylococcus aureus isolation. Results: For this, 400 cerebrospinal fluid samples were tested from hospitalized patients with arthritis in two hospitals in Urmia city during 3 months, which out of them 109 of samples were contaminated with bacteria including: 78 of isolates were Staphylococcus aureus, 12 of them were coagulase negative Staphylococci, 4 of them were Streptococcus and 15 of them were gram negative bacilli. Also, results showed that the amounts of glucose in positive samples in comparison to the amount of glucose in synovial fluid were significantly decreased. The amount of protein and the number of white blood cells in synovial fluid of positive samples were significantly higher in comparison to normal synovial fluid. Conclusion: Results showed that Staphylococcus aureus is the most common agent at infections arthritis, therefore it is recommended to use an experimental treatment for Staphylococcus aureus prior to final results.http://www.cjmb.org/uploads/pdf/pdf_CJMB_23.pdfSynovial fluidArthritisStaphylococcus aureusPCR. |
| spellingShingle | Afshin Taravati Afshin Zahedi Hiro Allipashaie Mehdi Dilmagani Identification of Staphylococcus aureus in Synovial Fluid of Patients Suspected to Arthritis Through PCR in Urmia City Crescent Journal of Medical and Biological Sciences Synovial fluid Arthritis Staphylococcus aureus PCR. |
| title | Identification of Staphylococcus aureus in Synovial Fluid of Patients Suspected to Arthritis Through PCR in Urmia City |
| title_full | Identification of Staphylococcus aureus in Synovial Fluid of Patients Suspected to Arthritis Through PCR in Urmia City |
| title_fullStr | Identification of Staphylococcus aureus in Synovial Fluid of Patients Suspected to Arthritis Through PCR in Urmia City |
| title_full_unstemmed | Identification of Staphylococcus aureus in Synovial Fluid of Patients Suspected to Arthritis Through PCR in Urmia City |
| title_short | Identification of Staphylococcus aureus in Synovial Fluid of Patients Suspected to Arthritis Through PCR in Urmia City |
| title_sort | identification of staphylococcus aureus in synovial fluid of patients suspected to arthritis through pcr in urmia city |
| topic | Synovial fluid Arthritis Staphylococcus aureus PCR. |
| url | http://www.cjmb.org/uploads/pdf/pdf_CJMB_23.pdf |
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