Expression of a truncated ATHB17 protein in maize increases ear weight at silking.
ATHB17 (AT2G01430) is an Arabidopsis gene encoding a member of the α-subclass of the homeodomain leucine zipper class II (HD-Zip II) family of transcription factors. The ATHB17 monomer contains four domains common to all class II HD-Zip proteins: a putative repression domain adjacent to a homeodomai...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3988052?pdf=render |
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| author | Elena A Rice Abha Khandelwal Robert A Creelman Cara Griffith Jeffrey E Ahrens J Philip Taylor Lesley R Murphy Siva Manjunath Rebecca L Thompson Matthew J Lingard Stephanie L Back Huachun Larue Bonnie R Brayton Amanda J Burek Shiv Tiwari Luc Adam James A Morrell Rico A Caldo Qing Huai Jean-Louis K Kouadio Rosemarie Kuehn Anagha M Sant William J Wingbermuehle Rodrigo Sala Matt Foster Josh D Kinser Radha Mohanty Dongming Jiang Todd E Ziegler Mingya G Huang Saritha V Kuriakose Kyle Skottke Peter P Repetti T Lynne Reuber Thomas G Ruff Marie E Petracek Paul J Loida |
| author_facet | Elena A Rice Abha Khandelwal Robert A Creelman Cara Griffith Jeffrey E Ahrens J Philip Taylor Lesley R Murphy Siva Manjunath Rebecca L Thompson Matthew J Lingard Stephanie L Back Huachun Larue Bonnie R Brayton Amanda J Burek Shiv Tiwari Luc Adam James A Morrell Rico A Caldo Qing Huai Jean-Louis K Kouadio Rosemarie Kuehn Anagha M Sant William J Wingbermuehle Rodrigo Sala Matt Foster Josh D Kinser Radha Mohanty Dongming Jiang Todd E Ziegler Mingya G Huang Saritha V Kuriakose Kyle Skottke Peter P Repetti T Lynne Reuber Thomas G Ruff Marie E Petracek Paul J Loida |
| author_sort | Elena A Rice |
| collection | DOAJ |
| description | ATHB17 (AT2G01430) is an Arabidopsis gene encoding a member of the α-subclass of the homeodomain leucine zipper class II (HD-Zip II) family of transcription factors. The ATHB17 monomer contains four domains common to all class II HD-Zip proteins: a putative repression domain adjacent to a homeodomain, leucine zipper, and carboxy terminal domain. However, it also possesses a unique N-terminus not present in other members of the family. In this study we demonstrate that the unique 73 amino acid N-terminus is involved in regulation of cellular localization of ATHB17. The ATHB17 protein is shown to function as a transcriptional repressor and an EAR-like motif is identified within the putative repression domain of ATHB17. Transformation of maize with an ATHB17 expression construct leads to the expression of ATHB17Δ113, a truncated protein lacking the first 113 amino acids which encodes a significant portion of the repression domain. Because ATHB17Δ113 lacks the repression domain, the protein cannot directly affect the transcription of its target genes. ATHB17Δ113 can homodimerize, form heterodimers with maize endogenous HD-Zip II proteins, and bind to target DNA sequences; thus, ATHB17Δ113 may interfere with HD-Zip II mediated transcriptional activity via a dominant negative mechanism. We provide evidence that maize HD-Zip II proteins function as transcriptional repressors and that ATHB17Δ113 relieves this HD-Zip II mediated transcriptional repression activity. Expression of ATHB17Δ113 in maize leads to increased ear size at silking and, therefore, may enhance sink potential. We hypothesize that this phenotype could be a result of modulation of endogenous HD-Zip II pathways in maize. |
| first_indexed | 2024-12-13T20:43:39Z |
| format | Article |
| id | doaj.art-7d41a4053b9f4bb494e4745c32c0ba55 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T20:43:39Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-7d41a4053b9f4bb494e4745c32c0ba552022-12-21T23:32:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9423810.1371/journal.pone.0094238Expression of a truncated ATHB17 protein in maize increases ear weight at silking.Elena A RiceAbha KhandelwalRobert A CreelmanCara GriffithJeffrey E AhrensJ Philip TaylorLesley R MurphySiva ManjunathRebecca L ThompsonMatthew J LingardStephanie L BackHuachun LarueBonnie R BraytonAmanda J BurekShiv TiwariLuc AdamJames A MorrellRico A CaldoQing HuaiJean-Louis K KouadioRosemarie KuehnAnagha M SantWilliam J WingbermuehleRodrigo SalaMatt FosterJosh D KinserRadha MohantyDongming JiangTodd E ZieglerMingya G HuangSaritha V KuriakoseKyle SkottkePeter P RepettiT Lynne ReuberThomas G RuffMarie E PetracekPaul J LoidaATHB17 (AT2G01430) is an Arabidopsis gene encoding a member of the α-subclass of the homeodomain leucine zipper class II (HD-Zip II) family of transcription factors. The ATHB17 monomer contains four domains common to all class II HD-Zip proteins: a putative repression domain adjacent to a homeodomain, leucine zipper, and carboxy terminal domain. However, it also possesses a unique N-terminus not present in other members of the family. In this study we demonstrate that the unique 73 amino acid N-terminus is involved in regulation of cellular localization of ATHB17. The ATHB17 protein is shown to function as a transcriptional repressor and an EAR-like motif is identified within the putative repression domain of ATHB17. Transformation of maize with an ATHB17 expression construct leads to the expression of ATHB17Δ113, a truncated protein lacking the first 113 amino acids which encodes a significant portion of the repression domain. Because ATHB17Δ113 lacks the repression domain, the protein cannot directly affect the transcription of its target genes. ATHB17Δ113 can homodimerize, form heterodimers with maize endogenous HD-Zip II proteins, and bind to target DNA sequences; thus, ATHB17Δ113 may interfere with HD-Zip II mediated transcriptional activity via a dominant negative mechanism. We provide evidence that maize HD-Zip II proteins function as transcriptional repressors and that ATHB17Δ113 relieves this HD-Zip II mediated transcriptional repression activity. Expression of ATHB17Δ113 in maize leads to increased ear size at silking and, therefore, may enhance sink potential. We hypothesize that this phenotype could be a result of modulation of endogenous HD-Zip II pathways in maize.http://europepmc.org/articles/PMC3988052?pdf=render |
| spellingShingle | Elena A Rice Abha Khandelwal Robert A Creelman Cara Griffith Jeffrey E Ahrens J Philip Taylor Lesley R Murphy Siva Manjunath Rebecca L Thompson Matthew J Lingard Stephanie L Back Huachun Larue Bonnie R Brayton Amanda J Burek Shiv Tiwari Luc Adam James A Morrell Rico A Caldo Qing Huai Jean-Louis K Kouadio Rosemarie Kuehn Anagha M Sant William J Wingbermuehle Rodrigo Sala Matt Foster Josh D Kinser Radha Mohanty Dongming Jiang Todd E Ziegler Mingya G Huang Saritha V Kuriakose Kyle Skottke Peter P Repetti T Lynne Reuber Thomas G Ruff Marie E Petracek Paul J Loida Expression of a truncated ATHB17 protein in maize increases ear weight at silking. PLoS ONE |
| title | Expression of a truncated ATHB17 protein in maize increases ear weight at silking. |
| title_full | Expression of a truncated ATHB17 protein in maize increases ear weight at silking. |
| title_fullStr | Expression of a truncated ATHB17 protein in maize increases ear weight at silking. |
| title_full_unstemmed | Expression of a truncated ATHB17 protein in maize increases ear weight at silking. |
| title_short | Expression of a truncated ATHB17 protein in maize increases ear weight at silking. |
| title_sort | expression of a truncated athb17 protein in maize increases ear weight at silking |
| url | http://europepmc.org/articles/PMC3988052?pdf=render |
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