Research Note: The prevalence and vertical transmission of avian hepatitis E virus novel genotypes in Tai'an city, China
ABSTRACT: To investigate the prevalence of avian hepatitis E virus (HEV) in chickens and gather evidence of viral vertical transmission, we collected 288 cloacal swabs and 288 yolks samples from 12 farms with clinically healthy chickens in 4 different areas in Tai'an City, Shandong Province, Ch...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122003923 |
| _version_ | 1811183555899817984 |
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| author | Kuihao Liu Fanliang Meng Jun Zhao Yiran Zhao Ningwei Geng Siqi Wang Liya Zhu Yufei Lou Mengda Liu Ning Li |
| author_facet | Kuihao Liu Fanliang Meng Jun Zhao Yiran Zhao Ningwei Geng Siqi Wang Liya Zhu Yufei Lou Mengda Liu Ning Li |
| author_sort | Kuihao Liu |
| collection | DOAJ |
| description | ABSTRACT: To investigate the prevalence of avian hepatitis E virus (HEV) in chickens and gather evidence of viral vertical transmission, we collected 288 cloacal swabs and 288 yolks samples from 12 farms with clinically healthy chickens in 4 different areas in Tai'an City, Shandong Province, China (i.e., Daiyue District, Xintai City, Feicheng City, and Ningyang County). We also collected 240 samples from 2 breeder farms (from each of which 30 chicks, 30 dead embryos, 30 live embryos, and 30 hatching eggs were taken). PCR detection revealed that the positive rates of cloacal swabs and yolks were 6.25% (18/288) and 4.51% (13/288), respectively. Besides, avian HEV was detected with higher positive rates in the chicks (11.67%), hatching eggs (10.00%), live embryos (13.33%), and dead embryos (26.67%) from 2 breeder farms. Sequence and genetic evolution analyses revealed that the nucleotide homology of the isolated strains was 76.4to 83.9% compared with 4 reported genotypes, but the isolated strains were located in a separate branch, indicating they were potential novel genotypes. In conclusion, those results indicate that the latent infection of avian HEV novel genotypes has been widespread in chicken farms in Tai'an City, and provide reliable evidence of the possible vertical transmission of avian HEV. |
| first_indexed | 2024-04-11T09:47:27Z |
| format | Article |
| id | doaj.art-7d41cc61f59747c6ad914ad7077c947c |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-11T09:47:27Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-7d41cc61f59747c6ad914ad7077c947c2022-12-22T04:30:55ZengElsevierPoultry Science0032-57912022-10-0110110102103Research Note: The prevalence and vertical transmission of avian hepatitis E virus novel genotypes in Tai'an city, ChinaKuihao Liu0Fanliang Meng1Jun Zhao2Yiran Zhao3Ningwei Geng4Siqi Wang5Liya Zhu6Yufei Lou7Mengda Liu8Ning Li9College of Animal Science and Technology, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271000, Shandong Province, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271000, Shandong Province, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271000, Shandong Province, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271000, Shandong Province, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271000, Shandong Province, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271000, Shandong Province, ChinaAnimal Husbandry and Veterinary Service Centre of Linshu, Linyi, 276700, Shandong Province, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271000, Shandong Province, ChinaChina Animal Health and Epidemiology Center, Qingdao, Shandong Province, 266032, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271000, Shandong Province, China; Corresponding author:ABSTRACT: To investigate the prevalence of avian hepatitis E virus (HEV) in chickens and gather evidence of viral vertical transmission, we collected 288 cloacal swabs and 288 yolks samples from 12 farms with clinically healthy chickens in 4 different areas in Tai'an City, Shandong Province, China (i.e., Daiyue District, Xintai City, Feicheng City, and Ningyang County). We also collected 240 samples from 2 breeder farms (from each of which 30 chicks, 30 dead embryos, 30 live embryos, and 30 hatching eggs were taken). PCR detection revealed that the positive rates of cloacal swabs and yolks were 6.25% (18/288) and 4.51% (13/288), respectively. Besides, avian HEV was detected with higher positive rates in the chicks (11.67%), hatching eggs (10.00%), live embryos (13.33%), and dead embryos (26.67%) from 2 breeder farms. Sequence and genetic evolution analyses revealed that the nucleotide homology of the isolated strains was 76.4to 83.9% compared with 4 reported genotypes, but the isolated strains were located in a separate branch, indicating they were potential novel genotypes. In conclusion, those results indicate that the latent infection of avian HEV novel genotypes has been widespread in chicken farms in Tai'an City, and provide reliable evidence of the possible vertical transmission of avian HEV.http://www.sciencedirect.com/science/article/pii/S0032579122003923avian hepatitis E virusyolkschick embryosubclinical infectionvertical transmission |
| spellingShingle | Kuihao Liu Fanliang Meng Jun Zhao Yiran Zhao Ningwei Geng Siqi Wang Liya Zhu Yufei Lou Mengda Liu Ning Li Research Note: The prevalence and vertical transmission of avian hepatitis E virus novel genotypes in Tai'an city, China Poultry Science avian hepatitis E virus yolks chick embryo subclinical infection vertical transmission |
| title | Research Note: The prevalence and vertical transmission of avian hepatitis E virus novel genotypes in Tai'an city, China |
| title_full | Research Note: The prevalence and vertical transmission of avian hepatitis E virus novel genotypes in Tai'an city, China |
| title_fullStr | Research Note: The prevalence and vertical transmission of avian hepatitis E virus novel genotypes in Tai'an city, China |
| title_full_unstemmed | Research Note: The prevalence and vertical transmission of avian hepatitis E virus novel genotypes in Tai'an city, China |
| title_short | Research Note: The prevalence and vertical transmission of avian hepatitis E virus novel genotypes in Tai'an city, China |
| title_sort | research note the prevalence and vertical transmission of avian hepatitis e virus novel genotypes in tai an city china |
| topic | avian hepatitis E virus yolks chick embryo subclinical infection vertical transmission |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122003923 |
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