Mutations of the c-KIT gene in canine mast cell tumors and respective nodal metastases classified according to mast cell infiltration
ABSTRACT: The molecular background of canine mast cell tumors (MCT) has been extensively investigated; however, the dynamic molecular changes that occur during carcinogenesis and metastasis are not fully understood. This study aimed to evaluate the incidence of mutations in the c-KIT proto-oncogene...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Patologia Animal (CBPA)
2023-03-01
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| Series: | Pesquisa Veterinária Brasileira |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2023000100504&tlng=en |
| _version_ | 1797858691964207104 |
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| author | Alice C. Rassele Larissa M. Souza Leonardo L. Gorza Antônio Giuliano Mayra C. Flecher Rodrigo S. Horta |
| author_facet | Alice C. Rassele Larissa M. Souza Leonardo L. Gorza Antônio Giuliano Mayra C. Flecher Rodrigo S. Horta |
| author_sort | Alice C. Rassele |
| collection | DOAJ |
| description | ABSTRACT: The molecular background of canine mast cell tumors (MCT) has been extensively investigated; however, the dynamic molecular changes that occur during carcinogenesis and metastasis are not fully understood. This study aimed to evaluate the incidence of mutations in the c-KIT proto-oncogene in canine MCTs and relative draining regional lymph nodes. Suspected or confirmed lymph node metastasis was classified accordingly to the HN Weishaar classification. The study included 34 dogs diagnosed with MCT; 19 patients were enrolled prospectively. These dogs had the primary MCT and regional lymph node resected and analyzed simultaneously. The second group was evaluated retrospectively and included fifteen patients resectioning the primary MCT without evaluation of regional lymph node. Analyzes of c-KIT mutation were performed for all primary MCTs and, in the first group, compared between primary MCT and HN-classified metastasis. Internal tandem duplications (ITD) in exon 11 of the c-KIT gene were detected in 20% of patients. Ten of the nineteen patients (52%) in the first group presented mast cell infiltration in the regional lymph node, and ITD in exon 11 of the c-KIT gene was detected in five and two dogs from Groups 1 and 2, respectively. ITD c-KIT mutations are common in canine MCT and may be found in the draining lymph node metastases/mast cell infiltrates in the absence of mutation of the primary tumor. Evaluation of c-KIT mutation in the primary tumor and metastases may be informative for defining both prognosis and therapeutic options in MCT cases. |
| first_indexed | 2024-04-09T21:17:29Z |
| format | Article |
| id | doaj.art-7d4297f95e7d4c2da011e6ae0bc562e6 |
| institution | Directory Open Access Journal |
| issn | 1678-5150 |
| language | English |
| last_indexed | 2024-04-09T21:17:29Z |
| publishDate | 2023-03-01 |
| publisher | Colégio Brasileiro de Patologia Animal (CBPA) |
| record_format | Article |
| series | Pesquisa Veterinária Brasileira |
| spelling | doaj.art-7d4297f95e7d4c2da011e6ae0bc562e62023-03-28T07:31:58ZengColégio Brasileiro de Patologia Animal (CBPA)Pesquisa Veterinária Brasileira1678-51502023-03-014310.1590/1678-5150-pvb-7140Mutations of the c-KIT gene in canine mast cell tumors and respective nodal metastases classified according to mast cell infiltrationAlice C. RasseleLarissa M. SouzaLeonardo L. Gorzahttps://orcid.org/0000-0001-9641-3325Antônio Giulianohttps://orcid.org/0000-0003-3993-038XMayra C. Flecherhttps://orcid.org/0000-0001-8403-538XRodrigo S. Hortahttps://orcid.org/0000-0002-9885-4498ABSTRACT: The molecular background of canine mast cell tumors (MCT) has been extensively investigated; however, the dynamic molecular changes that occur during carcinogenesis and metastasis are not fully understood. This study aimed to evaluate the incidence of mutations in the c-KIT proto-oncogene in canine MCTs and relative draining regional lymph nodes. Suspected or confirmed lymph node metastasis was classified accordingly to the HN Weishaar classification. The study included 34 dogs diagnosed with MCT; 19 patients were enrolled prospectively. These dogs had the primary MCT and regional lymph node resected and analyzed simultaneously. The second group was evaluated retrospectively and included fifteen patients resectioning the primary MCT without evaluation of regional lymph node. Analyzes of c-KIT mutation were performed for all primary MCTs and, in the first group, compared between primary MCT and HN-classified metastasis. Internal tandem duplications (ITD) in exon 11 of the c-KIT gene were detected in 20% of patients. Ten of the nineteen patients (52%) in the first group presented mast cell infiltration in the regional lymph node, and ITD in exon 11 of the c-KIT gene was detected in five and two dogs from Groups 1 and 2, respectively. ITD c-KIT mutations are common in canine MCT and may be found in the draining lymph node metastases/mast cell infiltrates in the absence of mutation of the primary tumor. Evaluation of c-KIT mutation in the primary tumor and metastases may be informative for defining both prognosis and therapeutic options in MCT cases.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2023000100504&tlng=enDogcanceroncogeneinternal tandem duplication |
| spellingShingle | Alice C. Rassele Larissa M. Souza Leonardo L. Gorza Antônio Giuliano Mayra C. Flecher Rodrigo S. Horta Mutations of the c-KIT gene in canine mast cell tumors and respective nodal metastases classified according to mast cell infiltration Pesquisa Veterinária Brasileira Dog cancer oncogene internal tandem duplication |
| title | Mutations of the c-KIT gene in canine mast cell tumors and respective nodal metastases classified according to mast cell infiltration |
| title_full | Mutations of the c-KIT gene in canine mast cell tumors and respective nodal metastases classified according to mast cell infiltration |
| title_fullStr | Mutations of the c-KIT gene in canine mast cell tumors and respective nodal metastases classified according to mast cell infiltration |
| title_full_unstemmed | Mutations of the c-KIT gene in canine mast cell tumors and respective nodal metastases classified according to mast cell infiltration |
| title_short | Mutations of the c-KIT gene in canine mast cell tumors and respective nodal metastases classified according to mast cell infiltration |
| title_sort | mutations of the c kit gene in canine mast cell tumors and respective nodal metastases classified according to mast cell infiltration |
| topic | Dog cancer oncogene internal tandem duplication |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2023000100504&tlng=en |
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