The Root of <i>Polygonum multiflorum</i> Thunb. Alleviates Non-Alcoholic Steatosis and Insulin Resistance in High Fat Diet-Fed Mice
Non-alcoholic steatosis and insulin resistance are critical health problems and cause metabolic complications worldwide. In this study, we investigated the molecular mechanism of <i>Polygonum multiflorum</i> Thunb. (PM) against hepatic lipid accumulation and insulin resistance by using i...
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MDPI AG
2020-08-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/8/2353 |
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| author | Soonwoong Jung Hyeonwi Son Chung Eun Hwang Kye Man Cho Sang Won Park Hwajin Kim Hyun Joon Kim |
| author_facet | Soonwoong Jung Hyeonwi Son Chung Eun Hwang Kye Man Cho Sang Won Park Hwajin Kim Hyun Joon Kim |
| author_sort | Soonwoong Jung |
| collection | DOAJ |
| description | Non-alcoholic steatosis and insulin resistance are critical health problems and cause metabolic complications worldwide. In this study, we investigated the molecular mechanism of <i>Polygonum multiflorum</i> Thunb. (PM) against hepatic lipid accumulation and insulin resistance by using in vitro and in vivo models. PM extract significantly attenuated the accumulation of lipid droplets and hepatic triglyceride in free fatty acid (FFA)-exposed HepG2 cells. PM extract increased the AMPK and ACC phosphorylation and GLUT4 expression, whose levels were downregulated in FFA-exposed cells. PM extract also decreased precursor and mature forms of SREBP-1 in FFA-exposed cells. C57BL/6 mice fed with normal diet (ND) or high-fat diet (HFD) were administered PM extract (100 mg/kg) or vehicle orally for 16 weeks. PM extract attenuated the increases of the epididymal and perirenal fats on HFD feeding. PM extract markedly reduced hepatic lipid accumulation and fasting glucose levels, and improved glucose and insulin sensitivity in HFD-fed mice. HFD-fed mice decreased the AMPK and ACC phosphorylation and GLUT4 expression, and increased precursor and mature forms of SREBP-1; these changes were significantly restored by PM extract. In conclusion, PM extract alleviates non-alcoholic steatosis and insulin resistance through modulating the expression of proteins on lipid metabolism and glucose transport in the liver. |
| first_indexed | 2024-03-10T17:51:54Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T17:51:54Z |
| publishDate | 2020-08-01 |
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| series | Nutrients |
| spelling | doaj.art-7d43a3bb908a42b19c2108d1a0d6d74f2023-11-20T09:20:17ZengMDPI AGNutrients2072-66432020-08-01128235310.3390/nu12082353The Root of <i>Polygonum multiflorum</i> Thunb. Alleviates Non-Alcoholic Steatosis and Insulin Resistance in High Fat Diet-Fed MiceSoonwoong Jung0Hyeonwi Son1Chung Eun Hwang2Kye Man Cho3Sang Won Park4Hwajin Kim5Hyun Joon Kim6Department of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 52727, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 52727, KoreaDepartment of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, KoreaDepartment of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, KoreaDepartment of Pharmacology and Convergence Medical Science, Bio Anti-aging Medical Research Center, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 52727, KoreaDepartment of Pharmacology and Convergence Medical Science, Bio Anti-aging Medical Research Center, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 52727, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 52727, KoreaNon-alcoholic steatosis and insulin resistance are critical health problems and cause metabolic complications worldwide. In this study, we investigated the molecular mechanism of <i>Polygonum multiflorum</i> Thunb. (PM) against hepatic lipid accumulation and insulin resistance by using in vitro and in vivo models. PM extract significantly attenuated the accumulation of lipid droplets and hepatic triglyceride in free fatty acid (FFA)-exposed HepG2 cells. PM extract increased the AMPK and ACC phosphorylation and GLUT4 expression, whose levels were downregulated in FFA-exposed cells. PM extract also decreased precursor and mature forms of SREBP-1 in FFA-exposed cells. C57BL/6 mice fed with normal diet (ND) or high-fat diet (HFD) were administered PM extract (100 mg/kg) or vehicle orally for 16 weeks. PM extract attenuated the increases of the epididymal and perirenal fats on HFD feeding. PM extract markedly reduced hepatic lipid accumulation and fasting glucose levels, and improved glucose and insulin sensitivity in HFD-fed mice. HFD-fed mice decreased the AMPK and ACC phosphorylation and GLUT4 expression, and increased precursor and mature forms of SREBP-1; these changes were significantly restored by PM extract. In conclusion, PM extract alleviates non-alcoholic steatosis and insulin resistance through modulating the expression of proteins on lipid metabolism and glucose transport in the liver.https://www.mdpi.com/2072-6643/12/8/2353high-fat dietinsulin resistancelipidnon-alcoholic steatosis<i>Polygonum multiflorum</i> |
| spellingShingle | Soonwoong Jung Hyeonwi Son Chung Eun Hwang Kye Man Cho Sang Won Park Hwajin Kim Hyun Joon Kim The Root of <i>Polygonum multiflorum</i> Thunb. Alleviates Non-Alcoholic Steatosis and Insulin Resistance in High Fat Diet-Fed Mice Nutrients high-fat diet insulin resistance lipid non-alcoholic steatosis <i>Polygonum multiflorum</i> |
| title | The Root of <i>Polygonum multiflorum</i> Thunb. Alleviates Non-Alcoholic Steatosis and Insulin Resistance in High Fat Diet-Fed Mice |
| title_full | The Root of <i>Polygonum multiflorum</i> Thunb. Alleviates Non-Alcoholic Steatosis and Insulin Resistance in High Fat Diet-Fed Mice |
| title_fullStr | The Root of <i>Polygonum multiflorum</i> Thunb. Alleviates Non-Alcoholic Steatosis and Insulin Resistance in High Fat Diet-Fed Mice |
| title_full_unstemmed | The Root of <i>Polygonum multiflorum</i> Thunb. Alleviates Non-Alcoholic Steatosis and Insulin Resistance in High Fat Diet-Fed Mice |
| title_short | The Root of <i>Polygonum multiflorum</i> Thunb. Alleviates Non-Alcoholic Steatosis and Insulin Resistance in High Fat Diet-Fed Mice |
| title_sort | root of i polygonum multiflorum i thunb alleviates non alcoholic steatosis and insulin resistance in high fat diet fed mice |
| topic | high-fat diet insulin resistance lipid non-alcoholic steatosis <i>Polygonum multiflorum</i> |
| url | https://www.mdpi.com/2072-6643/12/8/2353 |
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