Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel
The objective of this study is to test stem apex sizes in the in vitro establishing of Angelonia integerrima in order to obtain explants without by fungi and bacteria contamination for further multiplications. The treatments consisted of different stem apex sizes (1.0, 3.0, 5.0, 7.0, 9.0 and 11.0 m...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2018-10-01
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| Series: | Bioscience Journal |
| Subjects: | |
| Online Access: | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/39442 |
| _version_ | 1818156994961342464 |
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| author | Mara Cíntia Winhelmann Marília Tedesco Priscila Paris Eduarda Demari Avrella Claudimar Sidnei Fior Gilmar Schafer |
| author_facet | Mara Cíntia Winhelmann Marília Tedesco Priscila Paris Eduarda Demari Avrella Claudimar Sidnei Fior Gilmar Schafer |
| author_sort | Mara Cíntia Winhelmann |
| collection | DOAJ |
| description |
The objective of this study is to test stem apex sizes in the in vitro establishing of Angelonia integerrima in order to obtain explants without by fungi and bacteria contamination for further multiplications. The treatments consisted of different stem apex sizes (1.0, 3.0, 5.0, 7.0, 9.0 and 11.0 mm). At 45 and 90 days of cultivation, a count of contaminated explants and a count of shoots per explant formed were performed. In a second experiment, explants were cultivated in a medium containing different concentrations of benzylaminopurine (BAP) (0.0, 0.05, 0.10, 0.15 and 0.20 mg L-1). After 56 days of cultivation, the following variables were evaluated: shoot length, shoot fresh mass and number of shoots. During the explant establishment phase (45 days), only stem apexes with 1.0 mm in size were not contaminated. However, in the second subculture (at 90 days), only shoots from initial explants, with 7 mm in size or larger, were contaminated. Regarding multiplication, the presence of BAP showed a positive linear behavior for all variables. It is possible to obtain A. integerrima seedlings free of contamination in vitro by fungi and bacteria, using initial explants less than or equal to 5 mm. IBA provided a linear increment for the multiplication of this species.
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| first_indexed | 2024-12-11T15:07:09Z |
| format | Article |
| id | doaj.art-7d44ea0e64634222ab17f4c90f8fa1c5 |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-11T15:07:09Z |
| publishDate | 2018-10-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-7d44ea0e64634222ab17f4c90f8fa1c52022-12-22T01:00:51ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632018-10-01345Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel Mara Cíntia WinhelmannMarília TedescoPriscila ParisEduarda Demari AvrellaClaudimar Sidnei FiorGilmar Schafer The objective of this study is to test stem apex sizes in the in vitro establishing of Angelonia integerrima in order to obtain explants without by fungi and bacteria contamination for further multiplications. The treatments consisted of different stem apex sizes (1.0, 3.0, 5.0, 7.0, 9.0 and 11.0 mm). At 45 and 90 days of cultivation, a count of contaminated explants and a count of shoots per explant formed were performed. In a second experiment, explants were cultivated in a medium containing different concentrations of benzylaminopurine (BAP) (0.0, 0.05, 0.10, 0.15 and 0.20 mg L-1). After 56 days of cultivation, the following variables were evaluated: shoot length, shoot fresh mass and number of shoots. During the explant establishment phase (45 days), only stem apexes with 1.0 mm in size were not contaminated. However, in the second subculture (at 90 days), only shoots from initial explants, with 7 mm in size or larger, were contaminated. Regarding multiplication, the presence of BAP showed a positive linear behavior for all variables. It is possible to obtain A. integerrima seedlings free of contamination in vitro by fungi and bacteria, using initial explants less than or equal to 5 mm. IBA provided a linear increment for the multiplication of this species. https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/39442Pampa biomestem apexnative ornamentalPlantaginaceae |
| spellingShingle | Mara Cíntia Winhelmann Marília Tedesco Priscila Paris Eduarda Demari Avrella Claudimar Sidnei Fior Gilmar Schafer Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel Bioscience Journal Pampa biome stem apex native ornamental Plantaginaceae |
| title | Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
| title_full | Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
| title_fullStr | Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
| title_full_unstemmed | Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
| title_short | Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel |
| title_sort | clonal cleaning and in vitro multiplication of angelonia integerrima sprengel |
| topic | Pampa biome stem apex native ornamental Plantaginaceae |
| url | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/39442 |
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