QTL Pyramiding and Its Use in Breeding for Increasing the Phytoextraction Efficiency of Soil Cd via High-Cd-Accumulating Rice
Phytoextraction by high-Cd-accumulating rice lacking a functional <i>OsHMA3</i> allele is promising for Cd removal from paddy soils. To increase rice Cd extraction efficiency, we developed a new high-Cd variety, TJN25-11. For this, we pyramided a nonfunctional <i>OsHMA3</i> a...
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MDPI AG
2022-08-01
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| author | Tadashi Abe Masashi Ito Ryuichi Takahashi Toshimitsu Honma Masato Kuramata Satoru Ishikawa |
| author_facet | Tadashi Abe Masashi Ito Ryuichi Takahashi Toshimitsu Honma Masato Kuramata Satoru Ishikawa |
| author_sort | Tadashi Abe |
| collection | DOAJ |
| description | Phytoextraction by high-Cd-accumulating rice lacking a functional <i>OsHMA3</i> allele is promising for Cd removal from paddy soils. To increase rice Cd extraction efficiency, we developed a new high-Cd variety, TJN25-11. For this, we pyramided a nonfunctional <i>OsHMA3</i> allele from a high-Cd variety, Jarjan, and two QTLs for increased shoot Cd concentrations, which were discovered in a mapping population derived from a high-Cd variety, Nepal 555, and a low-Cd variety, Tachisugata. In two Cd-contaminated paddy fields under drained aerobic soil conditions, TJN25-11 presented significantly higher Cd concentrations in the straw and panicles than the OsHMA3-deficient varieties TJTT8 and Cho-ko-koku. Among the varieties, TJN25-11 had a relatively high shoot biomass, resulting in the highest Cd accumulation in the shoots. The soil Cd decreased by approximately 20% after TJN25-11 growth. The amount of Cd that accumulated in the TJN25-11 aerial parts was much greater than the amount of Cd that decreased in the topsoil, suggesting that Cd was absorbed from deeper soil layers. Thus, we revealed the effects of QTL pyramiding on shoot Cd accumulation and Cd phytoextraction efficiency. Since TJN25-11 has favorable agronomic traits for compatibility with Japanese cultivation systems, this variety could be useful for Cd phytoextraction in Cd-contaminated paddy fields. |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-09T09:50:04Z |
| publishDate | 2022-08-01 |
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| series | Plants |
| spelling | doaj.art-7d45ab2ddfd6413eb8b003a15dfe9af72023-12-02T00:11:22ZengMDPI AGPlants2223-77472022-08-011116217810.3390/plants11162178QTL Pyramiding and Its Use in Breeding for Increasing the Phytoextraction Efficiency of Soil Cd via High-Cd-Accumulating RiceTadashi Abe0Masashi Ito1Ryuichi Takahashi2Toshimitsu Honma3Masato Kuramata4Satoru Ishikawa5Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba 305-8604, JapanAkita Prefectural Agricultural Experiment Station, Akita 010-1231, JapanAkita Prefectural Agricultural Experiment Station, Akita 010-1231, JapanNiigata Agricultural Research Institute, Nagaoka 940-0826, JapanInstitute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba 305-8604, JapanInstitute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba 305-8604, JapanPhytoextraction by high-Cd-accumulating rice lacking a functional <i>OsHMA3</i> allele is promising for Cd removal from paddy soils. To increase rice Cd extraction efficiency, we developed a new high-Cd variety, TJN25-11. For this, we pyramided a nonfunctional <i>OsHMA3</i> allele from a high-Cd variety, Jarjan, and two QTLs for increased shoot Cd concentrations, which were discovered in a mapping population derived from a high-Cd variety, Nepal 555, and a low-Cd variety, Tachisugata. In two Cd-contaminated paddy fields under drained aerobic soil conditions, TJN25-11 presented significantly higher Cd concentrations in the straw and panicles than the OsHMA3-deficient varieties TJTT8 and Cho-ko-koku. Among the varieties, TJN25-11 had a relatively high shoot biomass, resulting in the highest Cd accumulation in the shoots. The soil Cd decreased by approximately 20% after TJN25-11 growth. The amount of Cd that accumulated in the TJN25-11 aerial parts was much greater than the amount of Cd that decreased in the topsoil, suggesting that Cd was absorbed from deeper soil layers. Thus, we revealed the effects of QTL pyramiding on shoot Cd accumulation and Cd phytoextraction efficiency. Since TJN25-11 has favorable agronomic traits for compatibility with Japanese cultivation systems, this variety could be useful for Cd phytoextraction in Cd-contaminated paddy fields.https://www.mdpi.com/2223-7747/11/16/2178ricecadmiumphytoextractionthree-way crossCd-accumulating abilityDNA marker-assisted breeding |
| spellingShingle | Tadashi Abe Masashi Ito Ryuichi Takahashi Toshimitsu Honma Masato Kuramata Satoru Ishikawa QTL Pyramiding and Its Use in Breeding for Increasing the Phytoextraction Efficiency of Soil Cd via High-Cd-Accumulating Rice Plants rice cadmium phytoextraction three-way cross Cd-accumulating ability DNA marker-assisted breeding |
| title | QTL Pyramiding and Its Use in Breeding for Increasing the Phytoextraction Efficiency of Soil Cd via High-Cd-Accumulating Rice |
| title_full | QTL Pyramiding and Its Use in Breeding for Increasing the Phytoextraction Efficiency of Soil Cd via High-Cd-Accumulating Rice |
| title_fullStr | QTL Pyramiding and Its Use in Breeding for Increasing the Phytoextraction Efficiency of Soil Cd via High-Cd-Accumulating Rice |
| title_full_unstemmed | QTL Pyramiding and Its Use in Breeding for Increasing the Phytoextraction Efficiency of Soil Cd via High-Cd-Accumulating Rice |
| title_short | QTL Pyramiding and Its Use in Breeding for Increasing the Phytoextraction Efficiency of Soil Cd via High-Cd-Accumulating Rice |
| title_sort | qtl pyramiding and its use in breeding for increasing the phytoextraction efficiency of soil cd via high cd accumulating rice |
| topic | rice cadmium phytoextraction three-way cross Cd-accumulating ability DNA marker-assisted breeding |
| url | https://www.mdpi.com/2223-7747/11/16/2178 |
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