Complete genome annotation data of Mycobacteriophages Prann and LeoAvram: New members of the family Siphoviridae
Two novel mycobacteriophages (Prann and LeoAvram) belonging to the family Siphoviridae were isolated from soil samples of Northern India. Genomic DNA of both the phages was extracted, and further sequenced using Illumina technology. Complete genome annotation of both the isolates was performed using...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340923002238 |
| _version_ | 1827919446686040064 |
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| author | Pragati Jamdagni Kiran Nehra J.S. Rana Louise M. Temple |
| author_facet | Pragati Jamdagni Kiran Nehra J.S. Rana Louise M. Temple |
| author_sort | Pragati Jamdagni |
| collection | DOAJ |
| description | Two novel mycobacteriophages (Prann and LeoAvram) belonging to the family Siphoviridae were isolated from soil samples of Northern India. Genomic DNA of both the phages was extracted, and further sequenced using Illumina technology. Complete genome annotation of both the isolates was performed using DNA Master. Prann and LeoAvram had linear genomes of 68398bp and 47079bp, respectively, with G+C contents of 60-70%. A total of 99 and 75 ORFs were predicted in Prann and LeoAvram, respectively. Based on sequence similarity to known phage proteins, functions were assigned to 44 and 53 genes, respectively. These proteins could be classified into five major groups, viz., phage structural proteins, proteins for recombination, lytic enzymes, proteins involved in DNA / RNA metabolism, and in regulation. Mycobacterium smegmatis was used in this work as a safe surrogate for Mycobacterium tuberculosis, the causative agent for tuberculosis, a major infectious disease worldwide with developing antibiotic resistance. This is the first report of M. smegmatis phages from Northern India. |
| first_indexed | 2024-03-13T03:58:04Z |
| format | Article |
| id | doaj.art-7d45d22f37eb476ebfb81fae2e7cdce1 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-03-13T03:58:04Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-7d45d22f37eb476ebfb81fae2e7cdce12023-06-22T05:03:35ZengElsevierData in Brief2352-34092023-06-0148109104Complete genome annotation data of Mycobacteriophages Prann and LeoAvram: New members of the family SiphoviridaePragati Jamdagni0Kiran Nehra1J.S. Rana2Louise M. Temple3Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal - 131039, Sonipat, Haryana, IndiaDepartment of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal - 131039, Sonipat, Haryana, India; Corresponding author.Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal - 131039, Sonipat, Haryana, IndiaSchool of Integrated Sciences, James Madison University, Harrisonburg, Virginia, USATwo novel mycobacteriophages (Prann and LeoAvram) belonging to the family Siphoviridae were isolated from soil samples of Northern India. Genomic DNA of both the phages was extracted, and further sequenced using Illumina technology. Complete genome annotation of both the isolates was performed using DNA Master. Prann and LeoAvram had linear genomes of 68398bp and 47079bp, respectively, with G+C contents of 60-70%. A total of 99 and 75 ORFs were predicted in Prann and LeoAvram, respectively. Based on sequence similarity to known phage proteins, functions were assigned to 44 and 53 genes, respectively. These proteins could be classified into five major groups, viz., phage structural proteins, proteins for recombination, lytic enzymes, proteins involved in DNA / RNA metabolism, and in regulation. Mycobacterium smegmatis was used in this work as a safe surrogate for Mycobacterium tuberculosis, the causative agent for tuberculosis, a major infectious disease worldwide with developing antibiotic resistance. This is the first report of M. smegmatis phages from Northern India.http://www.sciencedirect.com/science/article/pii/S2352340923002238Genome annotationBacteriophageDNA MasterGene functionMycobacterium smegmatis |
| spellingShingle | Pragati Jamdagni Kiran Nehra J.S. Rana Louise M. Temple Complete genome annotation data of Mycobacteriophages Prann and LeoAvram: New members of the family Siphoviridae Data in Brief Genome annotation Bacteriophage DNA Master Gene function Mycobacterium smegmatis |
| title | Complete genome annotation data of Mycobacteriophages Prann and LeoAvram: New members of the family Siphoviridae |
| title_full | Complete genome annotation data of Mycobacteriophages Prann and LeoAvram: New members of the family Siphoviridae |
| title_fullStr | Complete genome annotation data of Mycobacteriophages Prann and LeoAvram: New members of the family Siphoviridae |
| title_full_unstemmed | Complete genome annotation data of Mycobacteriophages Prann and LeoAvram: New members of the family Siphoviridae |
| title_short | Complete genome annotation data of Mycobacteriophages Prann and LeoAvram: New members of the family Siphoviridae |
| title_sort | complete genome annotation data of mycobacteriophages prann and leoavram new members of the family siphoviridae |
| topic | Genome annotation Bacteriophage DNA Master Gene function Mycobacterium smegmatis |
| url | http://www.sciencedirect.com/science/article/pii/S2352340923002238 |
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