Forskolin Improves Male Reproductive Complications Caused by Hyperglycemia in Type 2 Diabetic Rats
Background: In many diabetic patients, spermatogenesis complications are frequent causing infertility problems.This study aimed to demonstrate the effect of Forskolin on male reproductive dysfunction caused by type 2 diabetes.Materials and Methods: In this experimental study, type 2 diabetes was ind...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2023-10-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | https://www.ijfs.ir/article_253293_b736f6619d34b9118992d9de83833288.pdf |
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| author | Mehran Naghibi Hamid Tayefi Nasrabadi Jafar Soleimani Rad Alireza Garjani Mohammad Sadegh Gholami Farashah Daryoush Mohammadnejad |
| author_facet | Mehran Naghibi Hamid Tayefi Nasrabadi Jafar Soleimani Rad Alireza Garjani Mohammad Sadegh Gholami Farashah Daryoush Mohammadnejad |
| author_sort | Mehran Naghibi |
| collection | DOAJ |
| description | Background: In many diabetic patients, spermatogenesis complications are frequent causing infertility problems.This study aimed to demonstrate the effect of Forskolin on male reproductive dysfunction caused by type 2 diabetes.Materials and Methods: In this experimental study, type 2 diabetes was induced by a high-fat diet (HFD) for onemonth and then a low single dose injection (35 mg/kg) of streptozotocin (STZ) in Wistar rats. After 72 hours, rats withmore than 200 mg/dl of blood glucose were considered type 2 diabetic rats. Forty rats (200-250 g) were divided intofour groups (n=10) including group 1 (G1): rats with normal diet and buffer citrate (STZ solvent) injection, group 2(G2): control type 2 diabetic rats with HFD and STZ injection, group 3 (G3): type 2 diabetic rats received phosphatebuffer saline (PBS) as Forskolin solvent, and group 4 (G4): Forskolin treated diabetic rats (10 mg/kg) for 1 month.Results: In comparison to control group, in diabetic groups (G2 and G3) some parameters are increased significantly:The blood glucose (P=0.00078), testicular malondialdehyde (MDA) level and body weight (P=0.00009) and Baxgene expression (P=0.00007). Unlike, some parameters are decreased significantly: The serum level of testosterone(P=0.0009), testicular superoxide dismutase (SOD, P=0.00007) and glutathione peroxidase (GPX) levels (P=0.00008),sperm concentration (P=0.00008), motility (P=0.00009), normal morphological sperm (P=0.00008) and Bcl-2 geneexpression (P=0.00009). However, in Forskolin treated group (G4) the parameters stayed close to control values thatwas significantly (P=0.00007) higher than in G2 and G3 groups. Therefore, treatment with Forskolin significantlyimproved these abnormal changes in Forskolin-treated group.Conclusion: Our study demonstrates that Forskolin is an effective antidiabetic agent, which significantly improvessperm concentration, testosterone levels, and antioxidant activity in diabetic rats. |
| first_indexed | 2024-03-12T14:59:35Z |
| format | Article |
| id | doaj.art-7d49e423c997416c822e7c8635620bd7 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-03-12T14:59:35Z |
| publishDate | 2023-10-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-7d49e423c997416c822e7c8635620bd72023-08-14T09:37:54ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782023-10-0117426827510.22074/ijfs.2022.544368.1235253293Forskolin Improves Male Reproductive Complications Caused by Hyperglycemia in Type 2 Diabetic RatsMehran Naghibi0Hamid Tayefi Nasrabadi1Jafar Soleimani Rad2Alireza Garjani3Mohammad Sadegh Gholami Farashah4Daryoush Mohammadnejad5Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Pharmacology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranBackground: In many diabetic patients, spermatogenesis complications are frequent causing infertility problems.This study aimed to demonstrate the effect of Forskolin on male reproductive dysfunction caused by type 2 diabetes.Materials and Methods: In this experimental study, type 2 diabetes was induced by a high-fat diet (HFD) for onemonth and then a low single dose injection (35 mg/kg) of streptozotocin (STZ) in Wistar rats. After 72 hours, rats withmore than 200 mg/dl of blood glucose were considered type 2 diabetic rats. Forty rats (200-250 g) were divided intofour groups (n=10) including group 1 (G1): rats with normal diet and buffer citrate (STZ solvent) injection, group 2(G2): control type 2 diabetic rats with HFD and STZ injection, group 3 (G3): type 2 diabetic rats received phosphatebuffer saline (PBS) as Forskolin solvent, and group 4 (G4): Forskolin treated diabetic rats (10 mg/kg) for 1 month.Results: In comparison to control group, in diabetic groups (G2 and G3) some parameters are increased significantly:The blood glucose (P=0.00078), testicular malondialdehyde (MDA) level and body weight (P=0.00009) and Baxgene expression (P=0.00007). Unlike, some parameters are decreased significantly: The serum level of testosterone(P=0.0009), testicular superoxide dismutase (SOD, P=0.00007) and glutathione peroxidase (GPX) levels (P=0.00008),sperm concentration (P=0.00008), motility (P=0.00009), normal morphological sperm (P=0.00008) and Bcl-2 geneexpression (P=0.00009). However, in Forskolin treated group (G4) the parameters stayed close to control values thatwas significantly (P=0.00007) higher than in G2 and G3 groups. Therefore, treatment with Forskolin significantlyimproved these abnormal changes in Forskolin-treated group.Conclusion: Our study demonstrates that Forskolin is an effective antidiabetic agent, which significantly improvessperm concentration, testosterone levels, and antioxidant activity in diabetic rats.https://www.ijfs.ir/article_253293_b736f6619d34b9118992d9de83833288.pdfforskolinmale infertilityoxidative stresstesticular dysfunctiontype 2 diabetes |
| spellingShingle | Mehran Naghibi Hamid Tayefi Nasrabadi Jafar Soleimani Rad Alireza Garjani Mohammad Sadegh Gholami Farashah Daryoush Mohammadnejad Forskolin Improves Male Reproductive Complications Caused by Hyperglycemia in Type 2 Diabetic Rats International Journal of Fertility and Sterility forskolin male infertility oxidative stress testicular dysfunction type 2 diabetes |
| title | Forskolin Improves Male Reproductive Complications Caused by Hyperglycemia in Type 2 Diabetic Rats |
| title_full | Forskolin Improves Male Reproductive Complications Caused by Hyperglycemia in Type 2 Diabetic Rats |
| title_fullStr | Forskolin Improves Male Reproductive Complications Caused by Hyperglycemia in Type 2 Diabetic Rats |
| title_full_unstemmed | Forskolin Improves Male Reproductive Complications Caused by Hyperglycemia in Type 2 Diabetic Rats |
| title_short | Forskolin Improves Male Reproductive Complications Caused by Hyperglycemia in Type 2 Diabetic Rats |
| title_sort | forskolin improves male reproductive complications caused by hyperglycemia in type 2 diabetic rats |
| topic | forskolin male infertility oxidative stress testicular dysfunction type 2 diabetes |
| url | https://www.ijfs.ir/article_253293_b736f6619d34b9118992d9de83833288.pdf |
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