Immobilization of myoglobin in sodium alginate composite membranes
<title>Abstract</title><p>The immobilization of myoglobin in sodium alginate films was investigated with the aim of evaluating the protein stability in an ionic polymeric matrix. Myoglobin was chosen due to the resemblance to each hemoglobin tetramer. Sodium alginate, being a natur...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Polímeros
2015-06-01
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| Series: | Polímeros |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282015000300005&lng=en&tlng=en |
| _version_ | 1819141141105213440 |
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| author | Katia Cecília de Souza Figueiredo Wilbert van de Ven Matthias Wessling Tito Lívio Moitinho Alves Cristiano Piacsek Borges |
| author_facet | Katia Cecília de Souza Figueiredo Wilbert van de Ven Matthias Wessling Tito Lívio Moitinho Alves Cristiano Piacsek Borges |
| author_sort | Katia Cecília de Souza Figueiredo |
| collection | DOAJ |
| description | <title>Abstract</title><p>The immobilization of myoglobin in sodium alginate films was investigated with the aim of evaluating the protein stability in an ionic polymeric matrix. Myoglobin was chosen due to the resemblance to each hemoglobin tetramer. Sodium alginate, being a natural polysaccharide, was selected as the polymeric matrix because of its chemical structure and film-forming ability. To improve the mechanical resistance of sodium alginate films, the polymer was deposited over the surface of a cellulose acetate support by means of ultrafiltration. The ionic crosslink of sodium alginate was investigated by calcium ions. Composite membrane characterization comprised water swelling tests, water flux, SEM images and UV-visible spectroscopy. The electrostatic interaction between the protein and the polysaccharide did not damage the UV-visible pattern of native myoglobin. A good affinity between sodium alginate and cellulose acetate was observed. The top layer of the dense composite membrane successfully immobilized Myoglobin, retaining the native UV-visible pattern for two months.</p> |
| first_indexed | 2024-12-22T11:49:44Z |
| format | Article |
| id | doaj.art-7d4cd4d5a5524de9976028414e837a55 |
| institution | Directory Open Access Journal |
| issn | 1678-5169 |
| language | English |
| last_indexed | 2024-12-22T11:49:44Z |
| publishDate | 2015-06-01 |
| publisher | Associação Brasileira de Polímeros |
| record_format | Article |
| series | Polímeros |
| spelling | doaj.art-7d4cd4d5a5524de9976028414e837a552022-12-21T18:27:01ZengAssociação Brasileira de PolímerosPolímeros1678-51692015-06-0125326527010.1590/0104-1428.1818S0104-14282015000300005Immobilization of myoglobin in sodium alginate composite membranesKatia Cecília de Souza FigueiredoWilbert van de VenMatthias WesslingTito Lívio Moitinho AlvesCristiano Piacsek Borges<title>Abstract</title><p>The immobilization of myoglobin in sodium alginate films was investigated with the aim of evaluating the protein stability in an ionic polymeric matrix. Myoglobin was chosen due to the resemblance to each hemoglobin tetramer. Sodium alginate, being a natural polysaccharide, was selected as the polymeric matrix because of its chemical structure and film-forming ability. To improve the mechanical resistance of sodium alginate films, the polymer was deposited over the surface of a cellulose acetate support by means of ultrafiltration. The ionic crosslink of sodium alginate was investigated by calcium ions. Composite membrane characterization comprised water swelling tests, water flux, SEM images and UV-visible spectroscopy. The electrostatic interaction between the protein and the polysaccharide did not damage the UV-visible pattern of native myoglobin. A good affinity between sodium alginate and cellulose acetate was observed. The top layer of the dense composite membrane successfully immobilized Myoglobin, retaining the native UV-visible pattern for two months.</p>http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282015000300005&lng=en&tlng=enmyoglobinsodium alginateimmobilizationcomposite membranecellulose acetate |
| spellingShingle | Katia Cecília de Souza Figueiredo Wilbert van de Ven Matthias Wessling Tito Lívio Moitinho Alves Cristiano Piacsek Borges Immobilization of myoglobin in sodium alginate composite membranes Polímeros myoglobin sodium alginate immobilization composite membrane cellulose acetate |
| title | Immobilization of myoglobin in sodium alginate composite membranes |
| title_full | Immobilization of myoglobin in sodium alginate composite membranes |
| title_fullStr | Immobilization of myoglobin in sodium alginate composite membranes |
| title_full_unstemmed | Immobilization of myoglobin in sodium alginate composite membranes |
| title_short | Immobilization of myoglobin in sodium alginate composite membranes |
| title_sort | immobilization of myoglobin in sodium alginate composite membranes |
| topic | myoglobin sodium alginate immobilization composite membrane cellulose acetate |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282015000300005&lng=en&tlng=en |
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