Comparison of Two Different Canine Anti-IgG Antibodies for Assessment of Oligoclonal Bands in Cerebrospinal Fluid and Serum of Dogs via Isoelectric Focusing Followed by an Immunoblot
Isoelectric focusing followed by immunoblotting is a method routinely used in human medicine to assess the presence of oligoclonal bands (OCBs) in cerebrospinal fluid (CSF) and serum. The detection of OCBs is a valuable diagnostic test, especially important in patients with the suspicion of multiple...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.873456/full |
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| author | Julia K. Prümmer Veronika M. Stein Eliane Marti Mario Ziegler Andreas Lutterotti Ilijas Jelcic Frank Steffen Thorsten Buch Arianna Maiolini |
| author_facet | Julia K. Prümmer Veronika M. Stein Eliane Marti Mario Ziegler Andreas Lutterotti Ilijas Jelcic Frank Steffen Thorsten Buch Arianna Maiolini |
| author_sort | Julia K. Prümmer |
| collection | DOAJ |
| description | Isoelectric focusing followed by immunoblotting is a method routinely used in human medicine to assess the presence of oligoclonal bands (OCBs) in cerebrospinal fluid (CSF) and serum. The detection of OCBs is a valuable diagnostic test, especially important in patients with the suspicion of multiple sclerosis (MS), in which at least two OCBs are found in the CSF not present in paired serum samples in up to 95% of patients. So far, presence of OCBs in CSF and serum of dogs has only been investigated in a small cohort of dogs diagnosed with degenerative myelopathy and healthy dogs. The main objective of the current study was to describe the method used for OCB detection and compare two different canine anti-IgG antibodies: a canine rabbit-anti-IgG antibody (Jackson ImmunoResearch) vs. a canine goat-anti-IgG antibody (Bio-Rad). The method was performed according to the instructions of the commercial kit used. The canine goat-anti-IgG antibody showed a better performance than the canine rabbit-anti-IgG antibody. The availability of the technique of OCB detection in the dog paves the way for further studies, especially in the field of inflammatory diseases of the canine central nervous system, and comparison between specific human and canine diseases. |
| first_indexed | 2024-04-09T23:15:26Z |
| format | Article |
| id | doaj.art-7d4d04b4f0ee42799e144e8c772cb9db |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-09T23:15:26Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-7d4d04b4f0ee42799e144e8c772cb9db2023-03-22T09:49:22ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-07-01910.3389/fvets.2022.873456873456Comparison of Two Different Canine Anti-IgG Antibodies for Assessment of Oligoclonal Bands in Cerebrospinal Fluid and Serum of Dogs via Isoelectric Focusing Followed by an ImmunoblotJulia K. Prümmer0Veronika M. Stein1Eliane Marti2Mario Ziegler3Andreas Lutterotti4Ilijas Jelcic5Frank Steffen6Thorsten Buch7Arianna Maiolini8Clinical Neurology, Vetsuisse Faculty, University of Bern, Bern, SwitzerlandClinical Neurology, Vetsuisse Faculty, University of Bern, Bern, SwitzerlandDivision of Neurological Sciences – Clinical Immunology, Vetsuisse Faculty, University of Bern, Bern, SwitzerlandDepartment of Neurology, University of Zurich, Zurich, SwitzerlandDepartment of Neurology, University of Zurich, Zurich, SwitzerlandDepartment of Neurology, University of Zurich, Zurich, SwitzerlandClinical Neurology, Vetsuisse Faculty, University of Zurich, Zurich, SwitzerlandInstitute of Laboratory Animal Science, University of Zurich, Zurich, SwitzerlandClinical Neurology, Vetsuisse Faculty, University of Bern, Bern, SwitzerlandIsoelectric focusing followed by immunoblotting is a method routinely used in human medicine to assess the presence of oligoclonal bands (OCBs) in cerebrospinal fluid (CSF) and serum. The detection of OCBs is a valuable diagnostic test, especially important in patients with the suspicion of multiple sclerosis (MS), in which at least two OCBs are found in the CSF not present in paired serum samples in up to 95% of patients. So far, presence of OCBs in CSF and serum of dogs has only been investigated in a small cohort of dogs diagnosed with degenerative myelopathy and healthy dogs. The main objective of the current study was to describe the method used for OCB detection and compare two different canine anti-IgG antibodies: a canine rabbit-anti-IgG antibody (Jackson ImmunoResearch) vs. a canine goat-anti-IgG antibody (Bio-Rad). The method was performed according to the instructions of the commercial kit used. The canine goat-anti-IgG antibody showed a better performance than the canine rabbit-anti-IgG antibody. The availability of the technique of OCB detection in the dog paves the way for further studies, especially in the field of inflammatory diseases of the canine central nervous system, and comparison between specific human and canine diseases.https://www.frontiersin.org/articles/10.3389/fvets.2022.873456/fullcanine (dog)cerebrospinal fluid (CSF)inflammationimmunoblotisoelectric focusing (IEF) |
| spellingShingle | Julia K. Prümmer Veronika M. Stein Eliane Marti Mario Ziegler Andreas Lutterotti Ilijas Jelcic Frank Steffen Thorsten Buch Arianna Maiolini Comparison of Two Different Canine Anti-IgG Antibodies for Assessment of Oligoclonal Bands in Cerebrospinal Fluid and Serum of Dogs via Isoelectric Focusing Followed by an Immunoblot Frontiers in Veterinary Science canine (dog) cerebrospinal fluid (CSF) inflammation immunoblot isoelectric focusing (IEF) |
| title | Comparison of Two Different Canine Anti-IgG Antibodies for Assessment of Oligoclonal Bands in Cerebrospinal Fluid and Serum of Dogs via Isoelectric Focusing Followed by an Immunoblot |
| title_full | Comparison of Two Different Canine Anti-IgG Antibodies for Assessment of Oligoclonal Bands in Cerebrospinal Fluid and Serum of Dogs via Isoelectric Focusing Followed by an Immunoblot |
| title_fullStr | Comparison of Two Different Canine Anti-IgG Antibodies for Assessment of Oligoclonal Bands in Cerebrospinal Fluid and Serum of Dogs via Isoelectric Focusing Followed by an Immunoblot |
| title_full_unstemmed | Comparison of Two Different Canine Anti-IgG Antibodies for Assessment of Oligoclonal Bands in Cerebrospinal Fluid and Serum of Dogs via Isoelectric Focusing Followed by an Immunoblot |
| title_short | Comparison of Two Different Canine Anti-IgG Antibodies for Assessment of Oligoclonal Bands in Cerebrospinal Fluid and Serum of Dogs via Isoelectric Focusing Followed by an Immunoblot |
| title_sort | comparison of two different canine anti igg antibodies for assessment of oligoclonal bands in cerebrospinal fluid and serum of dogs via isoelectric focusing followed by an immunoblot |
| topic | canine (dog) cerebrospinal fluid (CSF) inflammation immunoblot isoelectric focusing (IEF) |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.873456/full |
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