Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway
We aim to investigate the effects of sevoflurane on the ATPase activity of the hippocampal neurons in rats with cerebral ischemia-reperfusion injury (IRI) via the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) signaling pathway. Sixty rats were assigned into the normal, model and s...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Kaohsiung Journal of Medical Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X17302802 |
| _version_ | 1818126360452792320 |
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| author | Tie-Jun Liu Jin-Cun Zhang Xiao-Zeng Gao Zhi-Bin Tan Jian-Jun Wang Pan-Pan Zhang Ai-Bin Cheng Shu-Bo Zhang |
| author_facet | Tie-Jun Liu Jin-Cun Zhang Xiao-Zeng Gao Zhi-Bin Tan Jian-Jun Wang Pan-Pan Zhang Ai-Bin Cheng Shu-Bo Zhang |
| author_sort | Tie-Jun Liu |
| collection | DOAJ |
| description | We aim to investigate the effects of sevoflurane on the ATPase activity of the hippocampal neurons in rats with cerebral ischemia-reperfusion injury (IRI) via the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) signaling pathway. Sixty rats were assigned into the normal, model and sevoflurane groups (n = 20, the latter two groups were established as focal cerebral IRI models). The ATPase activity was detected using an ultramicro Na (+)-K (+)-ATP enzyme kit. Immunohistochemical staining was used to detect the positive protein expression of cAMP and PKA. The hippocampal neurons were assigned to the normal, IRI, IRI + sevoflurane, IRI + forskolin, IRI + H89 and IRI + sevoflurane + H89 groups. qRT-PCR and Western blotting were performed for the expressions of cAMP, PKA, cAMP-responsive element-binding protein (CREB) and brain derived neurotrophic factor (BDNF). The normal and sevoflurane groups exhibited a greater positive protein expression of cAMP and PKA than the model group. Compared with the normal group, the expressions of cAMP, PKA, CREB and BDNF all reduced in the IRI, model and IRI + H89 groups. The sevoflurane group showed higher cAMP, PKA, CREB and BDNF expressions than the model group. Compared with the IRI group, ATPase activity and expressions of cAMP, PKA, CREB and BDNF all increased in the normal, IRI + sevoflurane and IRI + forskolin groups but decreased in the IRI + H89 group. It suggests that sevoflurane could enhance ATPase activity in hippocampal neurons of cerebral IRI rats through activating cAMP-PKA signaling pathway. |
| first_indexed | 2024-12-11T07:00:13Z |
| format | Article |
| id | doaj.art-7d4e2b76777d4fd6b3f23002d01add1c |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-12-11T07:00:13Z |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-7d4e2b76777d4fd6b3f23002d01add1c2022-12-22T01:16:38ZengWileyKaohsiung Journal of Medical Sciences1607-551X2018-01-01341223310.1016/j.kjms.2017.09.004Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathwayTie-Jun Liu0Jin-Cun Zhang1Xiao-Zeng Gao2Zhi-Bin Tan3Jian-Jun Wang4Pan-Pan Zhang5Ai-Bin Cheng6Shu-Bo Zhang7Department of Anesthesia, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR ChinaDepartment of Urology Surgery, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR ChinaDepartment of Anesthesia, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR ChinaDepartment of Anesthesia, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR ChinaDepartment of Critical Care Medicine, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR ChinaDepartment of Respiratory Medicine, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR ChinaDepartment of Critical Care Medicine, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR ChinaDepartment of Anesthesia, The Affiliated Hospital of North China University of Science and Technology, Tangshan, PR ChinaWe aim to investigate the effects of sevoflurane on the ATPase activity of the hippocampal neurons in rats with cerebral ischemia-reperfusion injury (IRI) via the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) signaling pathway. Sixty rats were assigned into the normal, model and sevoflurane groups (n = 20, the latter two groups were established as focal cerebral IRI models). The ATPase activity was detected using an ultramicro Na (+)-K (+)-ATP enzyme kit. Immunohistochemical staining was used to detect the positive protein expression of cAMP and PKA. The hippocampal neurons were assigned to the normal, IRI, IRI + sevoflurane, IRI + forskolin, IRI + H89 and IRI + sevoflurane + H89 groups. qRT-PCR and Western blotting were performed for the expressions of cAMP, PKA, cAMP-responsive element-binding protein (CREB) and brain derived neurotrophic factor (BDNF). The normal and sevoflurane groups exhibited a greater positive protein expression of cAMP and PKA than the model group. Compared with the normal group, the expressions of cAMP, PKA, CREB and BDNF all reduced in the IRI, model and IRI + H89 groups. The sevoflurane group showed higher cAMP, PKA, CREB and BDNF expressions than the model group. Compared with the IRI group, ATPase activity and expressions of cAMP, PKA, CREB and BDNF all increased in the normal, IRI + sevoflurane and IRI + forskolin groups but decreased in the IRI + H89 group. It suggests that sevoflurane could enhance ATPase activity in hippocampal neurons of cerebral IRI rats through activating cAMP-PKA signaling pathway.http://www.sciencedirect.com/science/article/pii/S1607551X17302802ATPase activitycAMP-PKA signaling pathwayCerebral ischemia-reperfusion injurySevofluraneHippocampal neuron |
| spellingShingle | Tie-Jun Liu Jin-Cun Zhang Xiao-Zeng Gao Zhi-Bin Tan Jian-Jun Wang Pan-Pan Zhang Ai-Bin Cheng Shu-Bo Zhang Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway Kaohsiung Journal of Medical Sciences ATPase activity cAMP-PKA signaling pathway Cerebral ischemia-reperfusion injury Sevoflurane Hippocampal neuron |
| title | Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway |
| title_full | Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway |
| title_fullStr | Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway |
| title_full_unstemmed | Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway |
| title_short | Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway |
| title_sort | effect of sevoflurane on the atpase activity of hippocampal neurons in a rat model of cerebral ischemia reperfusion injury via the camp pka signaling pathway |
| topic | ATPase activity cAMP-PKA signaling pathway Cerebral ischemia-reperfusion injury Sevoflurane Hippocampal neuron |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X17302802 |
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