Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth
Calmodulin-like (CML) proteins are primary calcium sensors and function in plant growth and response to stress stimuli. However, so far, the function of plant CML proteins, including tomato, is still unclear. Previously, it was found that a tomato (<i>Solanum lycopersicum</i>) CML, here...
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| author | Haidong Ding Ying Qian Yifang Fang Yurong Ji Jiarong Sheng Cailin Ge |
| author_facet | Haidong Ding Ying Qian Yifang Fang Yurong Ji Jiarong Sheng Cailin Ge |
| author_sort | Haidong Ding |
| collection | DOAJ |
| description | Calmodulin-like (CML) proteins are primary calcium sensors and function in plant growth and response to stress stimuli. However, so far, the function of plant CML proteins, including tomato, is still unclear. Previously, it was found that a tomato (<i>Solanum lycopersicum</i>) CML, here named <i>SlCML39</i>, was significantly induced by high temperature (HT) at transcription level, but its biological function is scarce. In this study, the characteristics of <i>SlCML39</i> and its role in HT tolerance were studied. <i>SlCML39</i> encodes a protein of 201 amino acids containing four EF hand motifs. Many cis-acting elements related to plant stress and hormone response appear in the promoter regions of <i>SlCML39</i>. <i>SlCML39</i> is mainly expressed in the root, stem, and leaf and can be regulated by HT, cold, drought, and salt stresses as well as ABA and H<sub>2</sub>O<sub>2</sub>. Furthermore, heterologous overexpression of <i>SlCML39</i> reduces HT tolerance in <i>Arabidopsis thaliana</i> at the germination and seedling growth stages. To better understand the molecular mechanism of <i>SlCML39</i>, the downstream gene network regulated by <i>SlCML39</i> under HT was analyzed by RNA-Seq. Interestingly, we found that many genes involved in stress responses as well as ABA signal pathway are down-regulated in the transgenic seedlings under HT stress, such as <i>KIN1</i>, <i>RD29B</i>, <i>RD26</i>, and <i>MAP3K18</i>. Collectively, these data indicate that <i>SlCML39</i> acts as an important negative regulator in response to HT stress, which might be mediated by the ABA signal pathway. |
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| language | English |
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| spelling | doaj.art-7d50cbc88e4849008742b4f1db2aecf32023-11-22T20:52:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122211147910.3390/ijms222111479Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling GrowthHaidong Ding0Ying Qian1Yifang Fang2Yurong Ji3Jiarong Sheng4Cailin Ge5Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaCollege of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaCollege of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaCollege of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaCollege of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaCalmodulin-like (CML) proteins are primary calcium sensors and function in plant growth and response to stress stimuli. However, so far, the function of plant CML proteins, including tomato, is still unclear. Previously, it was found that a tomato (<i>Solanum lycopersicum</i>) CML, here named <i>SlCML39</i>, was significantly induced by high temperature (HT) at transcription level, but its biological function is scarce. In this study, the characteristics of <i>SlCML39</i> and its role in HT tolerance were studied. <i>SlCML39</i> encodes a protein of 201 amino acids containing four EF hand motifs. Many cis-acting elements related to plant stress and hormone response appear in the promoter regions of <i>SlCML39</i>. <i>SlCML39</i> is mainly expressed in the root, stem, and leaf and can be regulated by HT, cold, drought, and salt stresses as well as ABA and H<sub>2</sub>O<sub>2</sub>. Furthermore, heterologous overexpression of <i>SlCML39</i> reduces HT tolerance in <i>Arabidopsis thaliana</i> at the germination and seedling growth stages. To better understand the molecular mechanism of <i>SlCML39</i>, the downstream gene network regulated by <i>SlCML39</i> under HT was analyzed by RNA-Seq. Interestingly, we found that many genes involved in stress responses as well as ABA signal pathway are down-regulated in the transgenic seedlings under HT stress, such as <i>KIN1</i>, <i>RD29B</i>, <i>RD26</i>, and <i>MAP3K18</i>. Collectively, these data indicate that <i>SlCML39</i> acts as an important negative regulator in response to HT stress, which might be mediated by the ABA signal pathway.https://www.mdpi.com/1422-0067/22/21/11479<i>Arabidopsis thaliana</i>calmodulin-like proteinhigh temperatureRNA sequencing<i>SlCML39</i>tomato |
| spellingShingle | Haidong Ding Ying Qian Yifang Fang Yurong Ji Jiarong Sheng Cailin Ge Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth International Journal of Molecular Sciences <i>Arabidopsis thaliana</i> calmodulin-like protein high temperature RNA sequencing <i>SlCML39</i> tomato |
| title | Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth |
| title_full | Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth |
| title_fullStr | Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth |
| title_full_unstemmed | Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth |
| title_short | Characteristics of <i>SlCML39</i>, a Tomato Calmodulin-like Gene, and Its Negative Role in High Temperature Tolerance of <i>Arabidopsis thaliana</i> during Germination and Seedling Growth |
| title_sort | characteristics of i slcml39 i a tomato calmodulin like gene and its negative role in high temperature tolerance of i arabidopsis thaliana i during germination and seedling growth |
| topic | <i>Arabidopsis thaliana</i> calmodulin-like protein high temperature RNA sequencing <i>SlCML39</i> tomato |
| url | https://www.mdpi.com/1422-0067/22/21/11479 |
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