Role of Periostin in Adhesion and Migration of Bone Remodeling Cells.
Periostin is an extracellular matrix protein highly expressed in collagen-rich tissues subjected to continuous mechanical stress. Functionally, periostin is involved in tissue remodeling and its altered function is associated to numerous pathological processes. In orthodontics, periostin plays key r...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4725750?pdf=render |
| _version_ | 1819108671857098752 |
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| author | Teresa Cobo Cristina G Viloria Laura Solares Tania Fontanil Elena González-Chamorro Félix De Carlos Juan Cobo Santiago Cal Alvaro J Obaya |
| author_facet | Teresa Cobo Cristina G Viloria Laura Solares Tania Fontanil Elena González-Chamorro Félix De Carlos Juan Cobo Santiago Cal Alvaro J Obaya |
| author_sort | Teresa Cobo |
| collection | DOAJ |
| description | Periostin is an extracellular matrix protein highly expressed in collagen-rich tissues subjected to continuous mechanical stress. Functionally, periostin is involved in tissue remodeling and its altered function is associated to numerous pathological processes. In orthodontics, periostin plays key roles in the maintenance of dental tissues and it is mainly expressed in those areas where tension or pressing forces are taking place. In this regard, high expression of periostin is essential to promote migration and proliferation of periodontal ligament fibroblasts. However little is known about the participation of periostin in migration and adhesion processes of bone remodeling cells. In this work we employ the mouse pre-osteoblastic MC3T3-E1 and the macrophage-like RAW 264.7 cell lines to overexpress periostin and perform different cell-based assays to study changes in cell behavior. Our data indicate that periostin overexpression not only increases adhesion capacity of MC3T3-E1 cells to different matrix proteins but also hampers their migratory capacity. Changes on RNA expression profile of MC3T3-E1 cells upon periostin overexpression have been also analyzed, highlighting the alteration of genes implicated in processes such as cell migration, adhesion or bone metabolism but not in bone differentiation. Overall, our work provides new evidence on the impact of periostin in osteoblasts physiology. |
| first_indexed | 2024-12-22T03:13:39Z |
| format | Article |
| id | doaj.art-7d50fe28239943a395b521cb2aa65872 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T03:13:39Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7d50fe28239943a395b521cb2aa658722022-12-21T18:40:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01111e014783710.1371/journal.pone.0147837Role of Periostin in Adhesion and Migration of Bone Remodeling Cells.Teresa CoboCristina G ViloriaLaura SolaresTania FontanilElena González-ChamorroFélix De CarlosJuan CoboSantiago CalAlvaro J ObayaPeriostin is an extracellular matrix protein highly expressed in collagen-rich tissues subjected to continuous mechanical stress. Functionally, periostin is involved in tissue remodeling and its altered function is associated to numerous pathological processes. In orthodontics, periostin plays key roles in the maintenance of dental tissues and it is mainly expressed in those areas where tension or pressing forces are taking place. In this regard, high expression of periostin is essential to promote migration and proliferation of periodontal ligament fibroblasts. However little is known about the participation of periostin in migration and adhesion processes of bone remodeling cells. In this work we employ the mouse pre-osteoblastic MC3T3-E1 and the macrophage-like RAW 264.7 cell lines to overexpress periostin and perform different cell-based assays to study changes in cell behavior. Our data indicate that periostin overexpression not only increases adhesion capacity of MC3T3-E1 cells to different matrix proteins but also hampers their migratory capacity. Changes on RNA expression profile of MC3T3-E1 cells upon periostin overexpression have been also analyzed, highlighting the alteration of genes implicated in processes such as cell migration, adhesion or bone metabolism but not in bone differentiation. Overall, our work provides new evidence on the impact of periostin in osteoblasts physiology.http://europepmc.org/articles/PMC4725750?pdf=render |
| spellingShingle | Teresa Cobo Cristina G Viloria Laura Solares Tania Fontanil Elena González-Chamorro Félix De Carlos Juan Cobo Santiago Cal Alvaro J Obaya Role of Periostin in Adhesion and Migration of Bone Remodeling Cells. PLoS ONE |
| title | Role of Periostin in Adhesion and Migration of Bone Remodeling Cells. |
| title_full | Role of Periostin in Adhesion and Migration of Bone Remodeling Cells. |
| title_fullStr | Role of Periostin in Adhesion and Migration of Bone Remodeling Cells. |
| title_full_unstemmed | Role of Periostin in Adhesion and Migration of Bone Remodeling Cells. |
| title_short | Role of Periostin in Adhesion and Migration of Bone Remodeling Cells. |
| title_sort | role of periostin in adhesion and migration of bone remodeling cells |
| url | http://europepmc.org/articles/PMC4725750?pdf=render |
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